Roles of microtubules and microtubule regulators in collective invasive migration of drosophila border cells

... phenotypes of stathminKO mutant animals 53   2.3.3 Roles of Stathmin in border cells .55   2.3.3.1 Effects of stathminKO on microtubules 55   2.3.3.2 Effects of stathminKO on border cell migration ... Autonomous and non-autonomous requirement of microtubules in border cell migration 105   VII 3.4 Interactions between microtubules and adhesions 106   3.5 R...

Ngày tải lên: 09/09/2015, 10:14

... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microt...

Ngày tải lên: 12/09/2015, 08:19

... center and plus-ends facing toward the cell tips Model of maintaining ordered microtubules (from Sawin and Tran, 20 06) is shown in the following cartoon 16 Model of interphase microtubules bundling ... critical role in maintaining cell morphology by binding to the plus ends of growing interphase microtubules in a Tip1p and Tea2p-dependent manner (Chang and Peter, 20 03)...

Ngày tải lên: 12/09/2015, 08:19

... 40μl TE buffer containing RNAse and incubated at 37 oC for 20min DNA was stored at -20oC 2 .3 Construction of knock out mutants and epitope tagging of genes 2 .3. 1 Construction of knock out mutants ... taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies 2.2.5 Extraction of S .pom...

Ngày tải lên: 12/09/2015, 08:19

... kinetochore in metaphase (~1% of cells) and to the spindle midzone during anaphase (~5% of cells) in control cells Ark1p-GFP defined the spindle midzone in 43 % of Mia1p- overexpressing cells, and ... panel) and cells overexpressing Mia1p (right panel) coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p- ov...

Ngày tải lên: 12/09/2015, 08:19

... interphase microtubule arrays 4 .5. 3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function has been illuminated by lack of large interphase MTOCs in ... that S pombe cells overexpressing the TACC- related protein, Mia1p, were capable of organizing spindle-pole like structures While formation of intranucl...

Ngày tải lên: 12/09/2015, 08:19

... spindle assembly checkpoint in fission yeast Nat Cell Biol 5, 764 - 766 ; author reply 766 68 Sato, M., and Toda, T (2007) Alp7 /TACC is a crucial target in Ran-GTPasedependent spindle formation in ... Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development Mech Dev 97, 13- 26 66 Samejima, I., Lourenco, P.C., Snait...

Ngày tải lên: 12/09/2015, 08:19

... key parameters in such a strategy remain ill defined, including the target MMP(s), timing and duration of intervention, specificity, dosage and delivery system A more rigorous understanding of ... permeability of the blood-brain barrier in inflammatory settings [52], which could be of relevance in SLE Matrix metalloproteinases and glomerulopathies Owing to overlapp...

Ngày tải lên: 09/08/2014, 13:22

... characteristics of solar cells having (a) AZO /p-type a-SiC:H and AZO/ p+ a-SiC:H /p-type a-SiC:H front interface and (b) AZO/ptype a-SiC:H and AZO/nc-Si /p-type a-SiC:H front interface Figure Dependence ... obtained only by engineering the interface between the p-type a-SiC:H and AZO and shows that a large increase in the solar cell efficiency can be...

Ngày tải lên: 20/06/2014, 20:20

... characteristics of solar cells having (a) AZO /p-type a-SiC:H and AZO/ p+ a-SiC:H /p-type a-SiC:H front interface and (b) AZO/ptype a-SiC:H and AZO/nc-Si /p-type a-SiC:H front interface Figure Dependence ... Interface modification effect between p-type a-SiC:H and ZnO:Al in p-i-n amorphous silicon solar cells Seungsin Baek*1, Jeong Chul Lee2,...

Ngày tải lên: 21/06/2014, 17:20

... PDZ -domain- containing (FRMPD2) [8] and Ras guanine exchange factor (RasGEF) veryKIND (v-KIND, or kinase noncatalytic C-lobe domain containing 1) [9] The KIND domain in these proteins is localized to the ... determined the structural and functional properties of the protein protein interaction between v-KIND and MAP2 We defined the binding core regi...

Ngày tải lên: 14/02/2014, 19:20

... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target...

Ngày tải lên: 16/02/2014, 09:20

... coronal section and scheme of the coronal section at E14.5 in (F) (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline Surprisingly, the ... binding is reduced, indicating that HSF4 facilitates HSF1 binding via chromatin remodelling Heat shock genes are not HSF4 targets, but HSF4 regulates a set of nonclassical h...

Ngày tải lên: 18/02/2014, 04:20

... both a novel C-terminal actin- binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actinbinding domain (this actin- binding domain has not yet been mapped) [23] We name the actin- binding domain that we have identified VH2-C and VH2-...

Ngày tải lên: 18/02/2014, 16:20

... HsRad51 to examine whether these loops are actually involved in DNA binding Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA- binding protein (SSB) and ... mutagenesis across the HsRad51 -L1 loop, and tested the interaction between the L1 loop and DNA A D B E Involvement of the L1 loop-Tyr232 residue in DNA...

Ngày tải lên: 19/02/2014, 06:20