GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... classification for dengue severity The new classification for dengue severity is divided into Dengue without Warning Signs, Dengue with Warning Signs, and Severe Dengue 24 1.2 Molecular Biology of DENV ... Fisher and Prof Leo Yee Sin, thank you for recruiting patients for our study To Prof Mary Ng and Boon, thank you for providing us with technical advice and reagents To Terence, thank you for your ... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101 Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and ...
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The generation of native human monoclonal antibodies with neutralising activity for dengue virus 2
... 1.1.5.4 Virus assembly and propagation 17 1.1.6 Phylogeny of Dengue Virus 19 1.1.7 Pathogenesis of Dengue Virus 22 1.1.8 Immune response to Dengue Virus 26 1.1.8.1 Innate immunity to DV 26 1.1.8 .2 ... culture 62 2.8 Source of primary CD 22+ B cells 63 2. 9 RNA extraction and RT-PCR for serotyping of patients 63 2. 10 Cloning of B cells from Dengue virus- infected patients 64 2. 10.1 Preparation of feeder ... 60 2. 2 EBV virus production 60 2. 3 Dengue virus production 60 2. 4 Cryopreservation of cells 61 2. 5 Hybridoma cultures and antibody purification 61 2. 6 Ascites and antibody purification 62 2.7...
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The generation of native human monoclonal antibodies with neutralising activity for dengue virus 3
... on the surface of the virus There are a number of different mechanisms postulated to prevent virus from entering the cell One way is for antibodies to alter the spatial distances 33 between the ... 6.0) of the trans Golgi network triggers dissociation of the prM/E heterodimers, which leads to the formation of 90 dimers that lie flat on the surface of the particle, with prM capping the fusion ... is a member of the Lymphocryptovirus genus, within the subfamily of Gammaherpesviruses, in the Herpesviridae family These viruses possess the ability to establish latent infection in the body throughout...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... al.: Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B Journal of Immune Based Therapies and Vaccines 2010 8:9 Submit your ... HuMAbs neutralize SEB-induced cytokine production by human lymphocytes To examine the biological activity of HuMAbs in vitro, a cell-based assay was employed that measures inhibitory Page of effects ... displayed the highest anti-SEB affinity, exhibited prophylactic as well as therapeutic activity in a mouse model of SEB-induced lethality Materials and methods Generation of HuMAbs Human B-cell hybridomas...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... hMnSOD has a low level of product inhibition, similar to that of the parent mutant Q143A hMnSOD, while exhibiting higher catalytic activity and efficiency Although even higher catalytic activity would ... efficiency and similarly low product inhibition compared with the Q143A hMnSOD parent Our results demonstrate the ability of directed evolution to engineer variants of hMnSOD with high catalytic activity ... anti-tumor effects of an active site mutant of human manganese- superoxide dismutase Evolutionary conservation of product inhibition J Biol Chem 279, 12769–12776 19 Bull C, Niederhoffer EC, Yoshida...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... degree of affinity and specificity for Tom70, and with a KD of nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the cytosolic domain is a potentially valuable tool in the ... one variant, designated 15Z-2, showed changes mapping to either the CDR or Ó FEBS 2003 An IgNAR variable domain specific for human Tom70 (Eur J Biochem 270) 3551 Fig Effect of removal of the 12F-11...
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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
... a single experiment only are shown in Figs 2,3,4 The importance of arginine residues in IS4VH As reported previously, the presence of the heavy chain of IS4 plays a dominant role in binding to ... 94 in CDR3 of UK4VL hinders DNA binding sterically A similar effect may be occurring with regards to the binding of UK4VL to CL The effect of point mutations of specific arginine residues in ... [27,37,41-43] In general, these studies have shown that altering the numbers of arginine residues in the CDRs of these antibodies can lead to significant alterations in binding to DNA Arginines in VH...
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Báo cáo y học: " Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural gene" pot
... Zohari et al., Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural ... of the influenza virus [19] The amino acid composition of the receptor binding pocket of the HA protein for the H10 isolates is typical of avian influenza viruses The H10 viruses have histidine ... factor other than the presence of multiple basic amino acids in the cleavage site contributed to the severity of H10 viruses in mink Neuraminidase Haemagglutinin Phylogenetic analysis of the HA...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... that one of the blocks to CYTOPLASM NUCLEUS Figure mutant 42-7 localization of proviral DNA in wild type and Synthesis andcells Synthesis and localization of proviral DNA in wild type and mutant ... efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend the rodent cell studies, and to identify cellular factors in human cells ... Pseudotyping human immunodeficiency virus type (HIV-1) by the glycoprotein of vesicular stomatitis virus targets HIV-1 entry to an endocytic pathway and suppresses both the requirement for Nef and...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... proximal tubular cells of human origin Therefore, human primary renal proximal tubular cells (HPTC) would be most suitable for such applications However, the application of primary human cells is ... effects on renal cells and possible applications will be discussed in detail 23 1.3 Genetic Engineering of HPTC and development of a BMP-7-producing BAK BMPs are members of the transforming growth ... cell-containing cartridges and BAKs in clinical trials These issues will be discussed in more details in the following section 1.2 Development of BAKs and applications of HPTC in such devices Acute renal...
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Identification and characterization of proteins that interact with zonula occludens proteins
... IDENTIFICATION AND CHARACTERIZATION OF PROTEINS THAT INTERACT WITH ZONULA OCCLUDENS PROTEINS P JAYA KAUSALYA (B.Sc (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... in TJ that are part of MAGUK family Fig 13: Structure of PDZ3 domain of PSD-95 with a peptide ligand Fig 14: Possible modes of interaction of PDZ containing proteins Fig 15: ZO-3 interacts with ... ZO-1, -2 and -3 to the actin cytoskeleton This study identifies the roles of zonula occludens (ZO) proteins and characterizes their interacting partners Novel proteins that interact with ZO-1...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... 2006 Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 Jain S K, Jain A, Hannurkar P R, Kotaiah S 2007 Characterization of plasma parameter, ... distance for (a) mirror magnetic field, and (b) flat magnetic field the magnetic field can cause diffusion of the plasma particles to the wall of the plasma chamber The measured magnetic field profiles along ... using standard relations for calculating magnetic field Design, fabrication, and characterization of a solenoid system 463 Table The optimized design parameters for solenoid coil with iron jacket...
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Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... astrocytoma grades I, II [astrocytoma], III [anaplastic astrocytoma] and IV [glioblastoma or GM]), oligodendrogliomas, ependymomas and mixed gliomas Gliomas constitute 77% of the primary malignant brain ... the characterization of a glioblastoma specific promoter This study deals with the identification, isolation and characterization of a glioblastoma specific promoter for suicide gene therapy ... 5'-flanking region of the IFI30 gene was taken for characterization for use as a glioblastoma specific promoter Deletion analysis of the promoter was done to check for the smallest length of the promoter...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... this article as: Lechner et al.: Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse ... serum and regulated by association of a latency protein, precluded clear neutralization data Characterization of human CD33+ and CD11b+ suppressor cells induced by tumor cell lines To characterize ... on a BD FACSCalibur flow cytometer and data acquisition and analysis were performed as above Data are from three unique donors and expressed as a fraction of labeled cells within a live -cell gate...
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Từ khóa: isolation and characterization of human fetal myoblastsexpression purification and characterization of single chain antibodiesexpression identification of il 18 producing cells and characterization of the periductal mononuclear cell infiltrates in salivary glands of patients with ss and controlscloning expression and characterization of a scfv f8 human il10 fusion proteinthe political economy and culture of human rights in east asiameaning and definition of human resource management pptanatomy and physiology of human heart pptevolution and development of human resource management pdfnature and value of human life christianityevolution and development of human resource management pptduties and responsibilities of human resource management assistantthermal oxide synthesis and characterization of fe3o4nanorods and fe2o3 nanowiresgrowth and development of human embryo and fetusisolation and characterization of vascular endothelial cells from murine heart and lungisolation and characterization of embryonic and adult epicardium and epicardium derived cellsNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ