Protein supplementation of human IVF culture media

Protein supplementation of human IVF culture media

... is universally added to most of human IVF culture media implies that it is widely considered to be of beneﬁt Indeed, the list of putative roles of albumin in culture media is extensive, including ... 3, March 2002 P1: GYQ Journal of Assisted Reproduction and Genetics pp399-jarg-368007 March 6, 2002 Protein Supplementation of Human IVF Culture Media potentia...

Ngày tải lên : 16/06/2015, 22:59

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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

... Purification and refolding of N-terminal His-tagged gAd-GLP-1-A fusion protein The fusion protein was purified by High-Affinity Ni-IDA Resin After filtering, the fusion protein was bound in the column ... by improving both insulin resistance and insulin secretion deficiency However, we only observed the glucose-lowering effect of the whole fusion protein in thi...

Ngày tải lên : 25/10/2012, 11:10

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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... FLAG -IST1 (WT) was clearly detected, but for FLAG–IST1DMIM1, FLAG–IST1DMIM2, and all MIM Fig Effects of mutations of IST1 MIMs on binding to mGFP calpain 7MIT mGFP calpain 7MIT and various FLAG IST1 ... proteins (GST– IST1, GST–IST1MIM, GST–IST1MIML326D,L353A, GST– CHMP6NT, GST–pHis and GST–MIT–pHis) Expression of GST IST1, GST–IST1MIM and GST–IST1L326D,L353A was induced with 0....

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: intersubunit interactions during virus assembly doc

... diverse CA–CA interactions and interfaces involved either in the assembly of the immature HIV-1 capsid, or in the reassembly of CA into a mature capsid The detailed description of the structure ... domains of the capsid ¨ protein of human immunodeﬁciency virus type J Virol 68, 8180–8187 ˚ ¨ Reicin AS, Ohagen A, Yin L, Hoglund S & Goff SP ¨ (1996) The r...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... structural studies on the capsid protein of the human immunodeficiency virus type 1: a new drug target? Sci World J 9, 404–419 Mateu MG (2009) The capsid protein of human immunodeficiency virus: intersubunit ... to the sequence of the dimerization helix of CTD (see above) CAI is the first reported peptide able to inhibit the assembly of the mature HI...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... iPLA2-VIA mediates U937 cell proliferation M A Balboa et al cPLA2 activity (% hydrolysis) A 0.0 0.2 0.4 0.6 0.8 1.0 [Pyrrophenone] (µM) B Number of cells × 105 iPLA2-VIA by treatment of cells ... the Ca2+dependent PLA2 activity of U937 cell homogenates The cell membrane assay was utilized (B) Time course of the effect of pyrrophenone on the proliferative capacity of U...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... resulting in the accumulation of both unmodiﬁed cholesteryl esters and glycated apoB in the cells The rate of removal of the latter species is slow, with only 20–30% of the glycated protein being ... although the concentrations of these materials present in the artery wall, and in atherosclerotic lesions, are unknown The role of glycation and...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... Structure of human wild-type lysozyme and location of the mutations discussed in this study The locations of the single-point mutations are shown on the structure of human wild-type lysozyme, deﬁned by ... demonstrate a clear relationship between the levels of protein secreted from P pastoris and the native-state thermostability of the lysozyme va...

Ngày tải lên : 19/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... 15 Ikeda Y, Okamura-Ikeda K & Tanaka K (1985) Puriﬁcation and characterization of short -chain, mediumchain, and long -chain acyl-CoA dehydrogenases from rat liver mitochondria: isolation of the ... (5¢-GAT AAAACCACACCTGTAGTAGCTG-3¢) and MCAD 116 5A G (5¢-CCTGTAGAAAGACTAATGAGGGATG CC-3¢) (mutagenic substitutions are shown in bold), and the antisense primer (5¢-GTAACGCCAGGGTT...

Ngày tải lên : 20/02/2014, 02:21

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Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: interactions of HIV-1 capsid with host protein factors ppt

... of HIV-1 capsid A Fig Role of TRIM5a and homology model of its PRYSPRY domain (A) The hypothesized role of TRIM5a in the HIV-1 viral life cycle (B) A homology model of the PRYSPRY domain of human ... interactions with other proteins that comprise the multi-synthetase complex [16] Based on the ﬁnding that VLPs composed only of HIV Gag protein package huma...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

... W303-1B (mat a, ade2 1, ura3 1, his3 11 , trp1 1, leu2–3 ,11 2, can1 10 0), FYF4-A1 aco1 (mat a, ura3–52, trp1D63, leu2D1, his3D200, aco1::URA3), and BY47 41 (mat a, his3, leu2, met15, ura3) were used in ... structure of human iron regulatory protein as cytosolic aconitase Structure 14 , 12 9 13 9 FEBS Journal 274 (2007) 10 83 10 92 ª 2007 The Authors Journal compilation ª 200...

Ngày tải lên : 07/03/2014, 10:20

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Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf

... GM2 and the interaction of the GM2AP ⁄ GM2 complex with the enzyme To delineate in detail the separate effect of the introduced mutations on the lipid- and the enzyme-binding of this cofactor, ... that of the WT protein Only these variants of GM2AP were examined in all further studies To assess the potential of the variant GM2AP forms for the stimulation...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khóa học: Some properties of human small heat shock protein Hsp20 (HspB6) potx

... of Hsp20, and both intact and Ó FEBS 2003 Human 20 kDa small heat shock protein (Eur J Biochem 271) 295 Fig Crosslinking of Hsp20 (A) Formation of disulﬁde crosslinked Hsp20 dimers A sample of ... plotted against the time of incubation Lack of precipitation of isolated small heat shock proteins is shown on curve 3D mutant of Hsp27 and wild-type Hsp20 (or...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the secondary structure of proteins ... spectrum of human PKIb in H2O The quantitative contributions of the indi...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... by DSC to be the Tm of the protein These ﬁbrils are amyloidogenic, as determined from their Stability and ﬁbril formation of TTR and TRP tinctorial properties in the ThT-binding and CR-binding ... with the thermostability of its tetrameric and monomeric structures Thermostability and protein structures We have analyzed the structures of hTTR (Protein...

Ngày tải lên : 16/03/2014, 01:20

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