IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

... poorly and has an increased affinity for the coupler/effector, even in the absence of agonist, thus leading to elevated basal signaling. This mutant form can nevertheless bind agonist and assume ... McMahon, Thomas Jessell and Phillip Beachy for comments on the manuscript. Shh-, Smo- and Ptc-transgenic lines were graciously provided by the labs of Andrew McMahon and Matthe...

Ngày tải lên: 06/08/2014, 18:20

... observation of two small 3 J HN-Ha cou- pling constants for residues Asn11 and Ser12 and the d NN(i,i +2) , d aN( i,i +2) [Ser12(C a H)–Trp14(NH) and Asn11(C a H)–Phe13(NH)] and d aN( i,i +3) [Asn11 (C a H)–Trp14(NH)] ... were assigned and inte- grated, with concomitant cycles of structure calcula- tions for evaluation of distance and angle constraint violations as...

Ngày tải lên: 18/02/2014, 17:20

... iturin As (heptapeptide consisting of six polar amino acids and a proline) and iturin Cs (heptapeptide consisting of five polar and an acidic amino acids as well as a proline). All the surfactins and ... & Castellino FJ (1998) Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen. Biochemistry 37 , 3258–3271. 29 Battist...

Ngày tải lên: 06/03/2014, 22:21

... subjected to TLC and quantification of radiolabelled ADP and AMP. The kinetics of [ 3 H]AMP and [ 3 H]ADP formation in the absence of tRNA Glu (black symbols) or of both L-Glu and tRNA Glu (grey ... substrates and inhibitors of Mt- GluRS: l-Glu, l-Gln and 2-oxoglutarate. Table S2. Alternate substrates and inhibitors of Mt- GluRS: ATP, AMP, pyrophosphate and thei...

Ngày tải lên: 07/03/2014, 03:20

... estimation of plasma Phe levels and ⁄ or Phe tolerance: 24 patients were classified as HPA I, 17 as HPA II and ten as HPA III. For nine patients (patients 3, 4, 5, 18, 25, 37, 39, 48 and 49), in whom ... text and Anna Nastasi for her skilful contribution to diet assistance in the diseased children. References 1 Scriver CR & Kaufman S (2001) Hyperphenylalanin- emia: phen...

Ngày tải lên: 16/03/2014, 01:20

... Sorescu et al. / Journal of Physics and Chemistry of Solids 65 (2004) 1021–1029 1025 [9] J. Cassedanne, An. Acad. Bras. Cienc. 38 (1966) 265. [10] H. Takano, Y. Bando, N. Nakanishi, M. Sakai, H. ... on both 57 Fe and 119 Sn isotopes have been performed for a better understanding of the site occupancy of Sn 4þ in the hematite lattice [12]. Finally, it was found that the degree...

Ngày tải lên: 19/03/2014, 16:48

... retent- ion times of inosine and hypoxantine, guanosine and guan- ine were 10.5 min and 4.7 min, and 11.5 min and 4.3 min, respectively. The amount of purine base formed is deter- mined by measuring the ... different substrate specificity of hPNP and hMTAP (Glu201, Asn243 and Val245 of hPNP instead of Ser178, Asp220 and Asp222 of hMTAP, respect- ively). The last imp...

Ngày tải lên: 23/03/2014, 09:21

... encephalo- pathy of cattle, chronic wasting disease in cervids, and Creutzfeldt–Jakob disease in humans. These diseases are characterized by astrocytic gliosis, neuronal apoptosis and deposition of an abnormally ... signal was removed from the spectra and peak fitting was performed using grams ai software (ThermoGalactic, Salem, NH). Lorentzian curves were fitted to the amide I band...

Ngày tải lên: 23/03/2014, 10:21

... for discussion and editing the manuscript and Dr Ch. Peters and Patric Hoert (University of Freiburg, Ger- many) for providing the Reflex III for measurements and for the mass spectrometry measurements. ... complex exists as a monomer and dimer and splits into a membrane and soluble subcomplexes. Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (...

Ngày tải lên: 23/03/2014, 13:20

... lic2A,RM118lpsA and RM118 lgtF before and after treatment with neuraminidase (as indicated by – and +, respectively). The sialylated tetrasaccharide-containing glycoforms are indicated by an asterisk. ... experiment [27] was acquired with a NOE mixing time of 800 ms, spin lock time of 80 ms, and 20480 transients for a duration of 21 h. Electrophoretic analysis of LPS LPSw...

Ngày tải lên: 23/03/2014, 21:21