báo cáo khoa học: " Characterisation of the tryptophan synthase alpha subunit in maize" pptx

... Site-specific mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium. Changing arginine 179 to leucine alters the reciprocal transmission of substrate- induced conformational ... localized in plastids, the major site of tryptophan biosynthesis in plants. It catalyzes the tryptophan synthase α-reaction (cleavage of IGP), and form...

Ngày tải lên: 12/08/2014, 05:20

... the truncation of the C-terminal domain in comparison to other SERK proteins. The actual function of the SERK C- terminal domain is unknown, but one possibility is a role in protein-protein interactions ... a predicted start codon in exon 9 in the region encoding subdomain IV of the the kinase domain. This sequence continues through to the position of the stop...

Ngày tải lên: 11/08/2014, 11:21

... frequency of RB is graphically exemplified in Fig. 1, show- ing that the frequency of RB is increasing by in- creased SCC and the increase is greater in larger herds than in smaller. As shown in Fig. ... re- flected in the present study showing that ani- mals getting their first insemination during Jan- uary have higher risk of becoming RB animals. In agreement with our...

Ngày tải lên: 12/08/2014, 15:20

... results in blocking of the binding of the SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For active n-Src in which the C-tail tyrosine was ... demonstrated that intra-domain interactions between Src family kinases (SFKs), stabilized by binding of the phosphorylated C-terminus to the SH2 domain and ⁄ or bindin...

Ngày tải lên: 06/03/2014, 01:20

... consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family. LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J subunits [2,3]. Apart from the asymmetry ... the interpretation of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma. The amount of PSI-G...

Ngày tải lên: 07/03/2014, 21:20

... fhx/P25 in the elementary unit in the presence of L-chain Two possibilities exist with respect to the role of L-chain in the protection of terminal a1,2-mannose residues of fhx/P25 in the elementary ... molecules of H-chains. We demon- strated previously that N-linked oligosaccharide chains of fhx/P25 were important in maintaining the integrity of the ele...

Ngày tải lên: 16/03/2014, 16:20

... of the membrane and, hence, the transbilayer pressure profile originating from the stiffening of lipid molecules in the polar but not in the acyl region of the membrane. (In this respect the in uence ... and 31 P-NMR data argue for the absence of CTII binding to Pam 2 Gro-PCho. The in uence of CTII binding to MLV of Pam 2 Gro- PGro on the lineshapes of...

Ngày tải lên: 17/03/2014, 03:20

... observed corresponding to the wild-type laccase from P. cinnabarinus. Immunodetection of the laccase was performed using antibodies raised against the P. cinnabarinus laccase. The Western blot ... character- ized in order to compare it’s properties to t hose of the wild- type protein. Two expression vectors were c onstructed containing the cDNA encoding the P. cinnabarinus...

Ngày tải lên: 17/03/2014, 11:20

... representation. Ala27 represents the N-terminus of the mature form of pFGE. The C-terminal residues 295–301 including PGEL are not visible in the crystal. The two calcium ions in each of the monomers are shown ... not affect binding of DDEL-containing ligands in vitro and in vivo, which suggests that different retrieval signals make different contacts in the bi...

Ngày tải lên: 23/03/2014, 07:20

... TMA-DPH located within the cells. The rapid initial quenching of the fluorescence was essentially complete within 1 s after the addition of TMRM. The extent of the initial quenching was linear with the outer ... surface of the cells, flip-flop across the plasma membrane, accu- mulation of dye in the cytoplasmic leaflet of the plasma membrane and release into t...

Ngày tải lên: 29/03/2014, 08:20