... Site-specific
mutagenesis of the alpha subunit of tryptophan synthase
from Salmonella typhimurium. Changing arginine 179 to
leucine alters the reciprocal transmission of substrate-
induced conformational ... localized in plastids,
the major site of tryptophan biosynthesis in plants. It catalyzes the tryptophan synthase α-reaction
(cleavage of IGP), and form...
... the
truncation of the C-terminal domain in comparison to
other SERK proteins. The actual function of the SERK C-
terminal domain is unknown, but one possibility is a role
in protein-protein interactions ... a predicted start codon in exon 9 in the region
encoding subdomain IV of the the kinase domain. This
sequence continues through to the position of the stop...
... frequency of
RB is graphically exemplified in Fig. 1, show-
ing that the frequency of RB is increasing by in-
creased SCC and the increase is greater in
larger herds than in smaller. As shown in Fig. ... re-
flected in the present study showing that ani-
mals getting their first insemination during Jan-
uary have higher risk of becoming RB animals.
In agreement with our...
... results in
blocking of the binding of the SH2 domain to the
substrate and thereby preventing interaction of
the substrate with the kinase domain. For active n-Src
in which the C-tail tyrosine was ... demonstrated that intra-domain interactions between
Src family kinases (SFKs), stabilized by binding of the phosphorylated
C-terminus to the SH2 domain and ⁄ or bindin...
... consisting of four members (Lhca1–4) of
the chlorophyll a ⁄ b binding protein family. LHCI is
bound along one side of PSI only, in the vicinity of
the PSI-F and PSI-J subunits [2,3]. Apart from the
asymmetry ... the
interpretation of their crystal structure of Pisum
sativum PSI, in which the loop of PSI-G is suggested
to face the stroma.
The amount of PSI-G...
... fhx/P25 in the elementary unit in the
presence of L-chain
Two possibilities exist with respect to the role of L-chain
in the protection of terminal a1,2-mannose residues of
fhx/P25 in the elementary ... molecules of H-chains. We demon-
strated previously that N-linked oligosaccharide chains of
fhx/P25 were important in maintaining the integrity of the
ele...
... of the membrane and, hence, the transbilayer
pressure profile originating from the stiffening of lipid
molecules in the polar but not in the acyl region of
the membrane. (In this respect the in uence ... and
31
P-NMR data argue for the absence of
CTII binding to Pam
2
Gro-PCho.
The in uence of CTII binding to MLV of Pam
2
Gro-
PGro on the lineshapes of...
... observed corresponding to the wild-type
laccase from P. cinnabarinus. Immunodetection of the
laccase was performed using antibodies raised against the
P. cinnabarinus laccase. The Western blot ... character-
ized in order to compare it’s properties to t hose of the wild-
type protein.
Two expression vectors were c onstructed containing the
cDNA encoding the P. cinnabarinus...
... representation. Ala27 represents the N-terminus of the
mature form of pFGE. The C-terminal residues 295–301 including
PGEL are not visible in the crystal. The two calcium ions in each of
the monomers are shown ... not affect
binding of DDEL-containing ligands in vitro and
in vivo, which suggests that different retrieval signals
make different contacts in the bi...
... TMA-DPH
located within the cells.
The rapid initial quenching of the fluorescence was
essentially complete within 1 s after the addition of
TMRM. The extent of the initial quenching was linear
with the outer ... surface of
the cells, flip-flop across the plasma membrane, accu-
mulation of dye in the cytoplasmic leaflet of the
plasma membrane and release into t...