Antibody Phage Display Methods and Protocols - part 5 docx

... 2,2′-di-p-nitrophenyl -5 , 5′,-diphenyl-3, 3 -[ 3,3′-dimethoxy-4,4′-diphenylene] ditetrazolium chloride and 0.38 mM 5- bromo-4-chloro-3-indoxyl phosphate mono-(p-toluidinium) salt in 0.1 M Tris- HCl, ... hapten; (5) and (6) unbound free and labeled hapten removed by washing. 187 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols...

Ngày tải lên: 10/08/2014, 00:21

... Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 1 Overview of Antibody Phage- Display Technology and Its Applications Hennie ... with complementary methods to increase the scope and range of applications of this technology. 2. Antibody Phage Display 2.1. The Phage- Display...

Ngày tải lên: 10/08/2014, 00:21

... 2 7-9 41 1-0 1); fl uorescein-isothiocyanate-conjugated anti-sheep Ab; anti-M13 Ab (Pharmacia Biotech cat. no. 2 7-9 41 0-0 1). 15. Tetramethyl-benzidine dihydrochloride (TMB) solution (Sigma, T86 65) ; ... OD 600 of 0 .5. Cell Surface Antigen-Binding scFvs 229 207 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P...

Ngày tải lên: 10/08/2014, 00:21

... 54 .0 57 .5 0.4 <0.1 0.12 0.10 0.36 2 .50 10 55 .0 <0.1 55 .0 59 .4 0.4 <0.2 0.12 0.10 0 .55 3. 85 15 57 .5 <0.1 57 .5 14.3 0.4 <0.3 High MgCl 2 0 .51 0.20 1. 15 3.76 15. 57 .5 <0.1 57 .5 ... 52 .0 <0.1 52 .0 53 .6 0.4 <0.1 0.22 0.20 0.34 2.36 15 52 .5 <0.1 52 .5 54.6 0.4 <0.1 0.23 0.20 0.42 2.90 16 53 .0 <0.1 53 .0 55 .5 0.4 <...

Ngày tải lên: 10/08/2014, 00:21

... (1997) Antibody- ribosome-mRNA (ARM) complexes as effi cient selection particles for in vitro display and evolution of antibody combining sites. Nucleic Acids Res. 25, 51 32 51 34. 86. Roberts, R. and ... circulating ligands. Nature Biotechnol. 15, 54 2 54 6. 116. Ridgway, J. B., Ng, E., Kern, J. A., Lee, J., Brush, J., Goddard, A., and Carter, P. (1999) Identifi cation of a h...

Ngày tải lên: 10/08/2014, 00:21

... CC-3′ HuV λ 3B-Back-APA 5 -ACC GCC TCC ACC AGT GCA CTT TCT TCT GAG CTG ACT CAG GAC CC-3′ HuV λ 4-Back-APA 5 -ACC GCC TCC ACC AGT GCA CAC GTT ATA CTG ACT CAA CCG CC-3′ HuV λ 5- Back-APA 5 -ACC ... 93 HuV κ 4B-Back-APA 5 -ACC GCC TCC ACC AGT GCA CTT GAT ATT GTG ATG ACC CAC ACT CC-3′ HuV κ 5- Back-APA 5 -ACC GCC TCC ACC AGT GCA CTT GAA ACG ACA CTC ACG CAG TCT CC-3′ HuV κ 6-Back-APA...

Ngày tải lên: 10/08/2014, 00:21

... ACC 133 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 9 Panning of Antibody Phage- Display ... solution. Panning Ab Phage- Display Libraries 141 147 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols E...

Ngày tải lên: 10/08/2014, 00:21

... immunohistochemistry and FACS (see Note 10). 242 Radoˇsevi´c and van Ewijk 255 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken ... had high CS-binding activity (see Notes 4 and 5) . In Vitro Mutagenesis of Ig Specifi city 263 2 45 From: Methods in Molecular Biology, vol. 178: Antibo...

Ngày tải lên: 10/08/2014, 00:21

... Ligation quantitation, 50 , 56 , 66, 75 E EGF, 22 Electroporation, methods, 52 , 66, 94, 293, 309, 314, 352 , 363, 3 65 preparation of cells, 51 , 99, 293, 309, 352 , 354 , 363 ELISA, antigen capture, 1 15, 1 95, 198 ... antigen, 6, 17, 18 CC-CKR5, 18 CCR5, 368 CD3, 319 CD19, 319 CD28, 319 CD36, 15 CD40, 121 CD 55, 17 cDNA, storage, 55 synthesis, 47, 65, 75, 83, 90, 97, 117, 126,...

Ngày tải lên: 10/08/2014, 00:21

... Differential Display 7 H-T110 H-T110 H-TIIA H-WA H-TIIA H-TIIC H-TllC H-AP3 H-APB H-API H-AP3 H-AP3 H-APl H-AP3 Fig. 2. Differential display using one-base anchored oligo-dT primers (7). ... Nucleic Acids Res 23, 452 0-4 52 3 10 Debouck, C (19 95) Differential display or differential dismay? Cur-r Opinzon Bzotechnol 6, 59 7 -5 99. Gene Cloning and Differential Display...

Ngày tải lên: 11/04/2014, 09:39