Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium" ppsx

... McFarland standard) was inoculated onto the surface of Brazier's agar and was then dried for 15 to 30 minutes. Etest strips were placed onto agar surface. Agar plates were incubated anaerobically ... analysis. RAS, LD, JP, MH and BWW drafted the manuscript. All authors contributed to and approved the final manuscript. Additional data files The following additional data...

Ngày tải lên: 09/08/2014, 20:20

... Australasian marsupials followed independent evolutionary pathways after the separation of Gondwana into the new continents of South America and Australia about 80 million years ago and after the ... contemporary continents of South America and Australia within Gondwanaland and the split of the American and Australian marsupials. Relative divergence in millions...

Ngày tải lên: 09/08/2014, 23:20

... Chandrasekaran A, Srinivasan A, Raman R, Viswana- than K, Raguram S, Tumpey TM, Sasisekharan V & Sasisekharan R (2008) Glycan topology determines human adaptation of avian H5N1 virus hemagglutinin. Nat ... degree and extent of branching and the relative abundance of a2 fi 3 and a2 fi 6 terminal sialic acids between cRBCs and HBEs, the present study provides insights...

Ngày tải lên: 14/02/2014, 19:20

... is a malate ion from the buffer in the active site of the enzyme. Malate mimics endo-oxabicyclic dicarboxylic acid, the transition state analog (TSA), in much the same fashion as citrate that ... cryo-data collection, the crystals were mounted through a layer of paraffin oil placed on top of the crystal- lization drop. The data were collected and processed with crys...

Ngày tải lên: 17/03/2014, 17:20

... 1211 Interestingly, the absence of subunit 6 also resulted in an increase in the ratio of intermediate to mature cyto- chrome c 1 and a disappearance of the intermediate form of the Rieske protein. At the same ... SDS/PAGE and Western blotting. SDS/PAGE and Western blotting Mitochondrial membranes were analyzed by standard SDS/PAGE with 15% (w/v) acrylamide and an...

Ngày tải lên: 19/02/2014, 12:20

... primer 5¢-GCAATCATATGAGCTGGAAGAGGAACAAGTT CCG-3¢ and the reverse primer 5¢-GGATCCTTAGGA GCTGAAAACCTCATCTGCG-3¢, containing NdeIand BamHI restriction sites, respectively. The resultant fragment was gel-purified ... thermodynamic and kinetic properties of the isolated FAD domain of rat nNOS and three mutant forms. The isolated FAD domains have enabled: (a) potentiometric analy...

Ngày tải lên: 30/03/2014, 14:20

... free-living taxa to the Strongylida are members of the Rhabditina, including C. elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence analysis [48]. A more ... J, Williams SA, Lustigman S: Identification of potential vaccine and drug target candidates by expressed sequence tag analysis and immunoscreening of Onchoce...

Ngày tải lên: 09/08/2014, 20:20

... oligosaccharide anthracycline, and an antineoplastic antibiotic. It can intercalate into DNA and interact with topoisomerase I and II, thereby inhibiting DNA replication and DNA repair [11]. This agent ... and analysis, drafting and critically revising the manuscript. All authors are involved in reviewing and revising the manuscript. All authors have read and approve...

Ngày tải lên: 10/08/2014, 21:23

... purposes of learning English and their participation in learning to speak English 21 Table 3: Teachers’ and students’ attitudes towards speaking materials and appropriate time for teaching and learning ... cộng tác c a các bạn! v LIST OF TABLES AND CHARTS Tables: Table 1: Teachers’ and students’ attitudes towards speaking skill and the role of teaching and learning...

Ngày tải lên: 07/11/2012, 14:50

... learning a new language so that they can have more advantages in running their business. Nevertheless, most of language learners have the same obstacle in comparing and contrasting their mother ... English and Vietnamese and thus, suggest some application for language teachers and learners. Nguyễn Thanh Thảo Nguyen 12 questions. Instead, intonation and interrogative operat...

Ngày tải lên: 22/01/2013, 09:28