subjectivity and sentiment analysis of modern standard arabic

... for analysis The lower limit for band detection was set by a relative intensity per lean of 0.5% of the total band intensity The presence or absence of bands in fingerprints of test systems A and ... labels a, b, and c are PCR-DGGE banding patterns of 16S rRNA gene in test systems A, B, and C, respectively; d, e, and f are PCR-DGGE banding patterns of C12O gene in test systems A, B, and C, respectively; ... 15 15 Fig - PCR-DGGE banding patterns of 16S rRNA gene, C12O gene and C23O gene of Spirodela polyrrhiza-associated bacterial communities in test systems A and B and that of suspended bacterial

Ngày tải lên: 05/09/2013, 10:15

... identification of genuine 14-3-3 ligands. Detailed analysis of the 14-3-3-asociated proteins found showed that 46 of them were exclusively present in one of the conditions ana- lyzed and 15 of them ... percentage of breast tumors in the early stage of the disease, contributing to the transformation of cells and also to the further progression of breast cancer [42]. On the other hand, downregulation of ... signal trans- ducer and activator of transcription 3 (STAT3), and hematopoietic progenitor kinase 1-interacting protein of 55 kDa (HIP-55). Using both MS and MALDI- TOF ⁄ TOF MS tryptic mass fingerprinting,

Ngày tải lên: 06/03/2014, 22:21

... require individual diagnosis and therapy, and an understand- ing of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment. ... Taiwan 3 Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan 4 Department of Pediatric, Division of Immunology and Allergy, University of Florida, Gainesville, ... 3. cDNA sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide

Ngày tải lên: 07/03/2014, 12:20

... the pK a of the catalytic acid (Glu144) is ÔcycledÕ during catalysis as a consequence of substrate-binding and release and, possibly, by a back and forth movement of Asp142 between Asp140 and Glu144. Rotation ... pH 8.0, 8.3 and 9.0 bicine/HCl buffer and pH 9.0, 9.5 and 10.0 ethanolamine/ HCl buffer [36]. Structural analysis and electrostatics calculations Studies of chitinase structures and molecular ... moves into the ÔupÕ position and interacts with the substrate and Glu144. This rotation of Asp142 is accompanied by removal of a water molecule and adjustments of Tyr10 and Ser93 that seem to compensate

Ngày tải lên: 07/03/2014, 14:20

... Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction. The interaction of Pex1p and Pex6p involves their ... biological function of Pex1p and Pex6p. A B Fig. 2. Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites. A schematic representation of the ... parts of the N-terminal fragment and ⁄ or the second cassette; AB DC EF Fig. 5. Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex6p on the morphological appearance of

Ngày tải lên: 07/03/2014, 16:20

... BSA as the standard. The cobalt and iron contents were determined by ICP-AES (SPS-1200 V; Seiko Instruments, Chiba, Japan) using sample solutions, and standard solutions of cobalt and iron (Wako ... and Fe-type NHase are shown in yellow, red and cyan, respectively. The Cc2atomofaThr109 and the Cc1atomofaVal136 of the Co-type NHase are connected by a yellow line. (B) Vicinity of aTyr114 of ... residue in the aT109S and aY114T structures, and a cobalt atom and three oxygen atoms in the apoenzyme and aY114T structures. Several rounds of refinement and model Ó FEBS 2003 Mutants of Co-type NHase

Ngày tải lên: 16/03/2014, 16:20

... regulating IL-18 expression and processing in this species, the first report of such a phenomenon for this cytokine Materials and methods Cloning and sequencing of genomic DNA and cDNA All products ... with an equal volume of phenol/chloroform (24 : 1, v/v) and once with chloroform Genomic DNA was precipitated with two volumes of cold ethanol, washed with 70% (v/v) ethanol, and dissolved in TE ... the human IL-18 gene, consisting of six exons and five introns The Fig Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the predicted Fugu/tetraodon

Ngày tải lên: 16/03/2014, 16:20

... spectrum of the protein excited at 300 nm with that of the standard N-Ac-Trp-NH 2 normalized to the same absorbance at 300 nm. A value of 0.13 was used for the quantum yield of the standard [28]. ... (K 2 0.667 [30]). A value of 1.36 was taken for the refractive index n [31]. MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after tryptic digestion of the labeled and nonlabeled Ag-NPA-1 ... incorporation of the ligand. Binding of DAUDA to Ag-NPA-1 caused  20% quenching of the emission of Trp17 indicating FRET between the single Trp residue and the dansyl group. We calculated a value of 3.74

Ngày tải lên: 16/03/2014, 18:20

... addresses *Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea Division of Molecular Biology, Department of Zoology, Sri Venkateswara ... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein. Results and Discussion Annotation of CMs in M. tuberculosis ... alignment that helix 3 of 90-MtCM is a shorter version of helix 3 of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig. 4C) corre- sponding to Ser84 and Gln88 of EcCM as discussed below. During

Ngày tải lên: 17/03/2014, 17:20

... organization of this gene and analyzed its structural evolution. Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp (Cc ... Additionally, the burst of determina- tion of genome sequences in recent years has led to identification of homologs of many ribonucleases in a variety of diverse organisms. Many close homologs of bacterial ... all insect tissues and developmental stages. Sequence analysis of the extended region of the longer transcript revealed the existence of three mRNA instability motifs (AUUUA) and five poly(U) tracts,

Ngày tải lên: 23/03/2014, 06:20

... G or C. Analysis of DNA binding by hydroxyl radical footprinting and interference assays A more detailed picture of the binding of HAKN1 to its target site was obtained by the analysis of footprint- ing ... panels in (A) and (B) represent the top and bottom strands of the binding site, respectively. A portion of the same fragment digested with defined restriction enzymes was used as a standard to calculate ... incorporation of Ile50 and Arg55 must have been particularly important. Other homeodomains that contain Ile50 and Arg55 are those of the TGIF, Meis and Bell families [22] and yeast MATa1 [36]. TGIF and

Ngày tải lên: 23/03/2014, 13:20

... financial analysis is usually referred as the viability, stability and profitability of a business and business analysis usually... Business and Financial Analysis of BP ... analysis of ratios breakeven analysis and various trends 24 Business and Financial Analysis of BP with comparison to Shell PLC 2011 The overall financial ratio analysis ... and the Shell PLC are... rudiments and icons of the business and financial analysis, there effects and significance in the pertinent field 17 Business and Financial Analysis

Ngày tải lên: 25/03/2014, 11:06

... tuberculosis skin testing and LTBI treatment, the prevalence and outcome of MTB infections, and the effects of patient factors and treatment on mortality from MTB infection PATIENTS AND METHODS plant ... Alto, CA; and Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Arizona, Tucson, AZ Mycobacterium tuberculosis (MTB) causes substantial morbidity and mortality ... rate of MTB reactivation,12 some transplant centers neither test for nor treat LTBI because of the perceived lack of efficacy and potential toxicity of drug therapy in liver transplant candidates.13-18

Ngày tải lên: 29/03/2014, 03:20

... results of the luciferase activity assays for different constructs in A549 and DU145 cells. (C) The effects of 2 lg of the NEG-U decoy, 2 lg of the NEG-D decoy and a combination of 1 lg of each ... between the NEG-U and NEG-D elements, 2 lg of the NEG-U decoy, 2 lg of the NEG-D decoy, and a combination of 1 lg of each of the decoys were used, and the above-mentioned ... decoy, 2 lg of the NEG-D decoy, and a combination of 1 lg each of the NEG-U and NEG-D decoys. Our results demonstrated that the F-560 activity is increased more by the combination of the NEG-U and NEG-D

Ngày tải lên: 30/03/2014, 04:20

... stages of the plant life cycle [16–21] In this study, the genomic sequences of the cardosin A and B genes and of two new cardosin genes (those encoding cardosins C and D) were isolated and characterized ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of ... alignment and homology of cardosins A, B, C and D and cyprosin A and B (A) The amino acid sequences were deduced from the genomic sequences (this work), with the exception of cyprosin A (X69193) and

Ngày tải lên: 30/03/2014, 09:20

... in a total volume of 100 lL, comprising 2 lLof25mm dNTP mixture, 2 lLof20lm primer solution, 1 U of DeepVent DNA polymerase (New England Biolabs, Hitchin, England) and 100 ng of the tem- plate ... Fernando LP & Andrews GK (1989) Cloning and expression of an avian metallothionein- encoding gene Gene 81, 177–183 Fernando LP, Wei D & Andrews GK (1989) Structure and expression of ... weights of zinc and copper and the ESI-MS experimental error range prevented determination of the Zn:Cu ratio in the heterometallic Zn,Cu–ckMT species. Zn(II) and Cd(II) binding abilities of ckMT and

Ngày tải lên: 30/03/2014, 11:20

... determination of the midpoint reduction potentials of flavin cofactors, and also of the haems in the case of NOS and P450 BM3 In spectroelectrochemical titrations of the isolated domains, the lack of overlapping ... potentiometric analysis of the wild-type and mutant proteins to probe any thermodynamic consequences of mutation; and (b) studies of the kinetics of flavin reduction by reducing coenzymes in the absence of ... presence of CaM, through release of the NADPH-dependent conformational lock [16] Of the three NOS isoforms, the inducible NOS isoform is expressed with CaM tightly bound [14] and regulation of activity

Ngày tải lên: 30/03/2014, 14:20

... residues of D3 and B contribute to the stability of the dimer The first cluster includes F70/V336 and I72/Q338 (both in b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 ... b5 strand) of D3 and I41/L304, C43/A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 ... in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 (b2 strand), I33/K294 (loop after b2 strand), I68/F334, L71/ V337 and L73/F339

Ngày tải lên: 30/03/2014, 15:20

... of the results of the mechanical and metallurgical analysis of structural steel from the World Trade Center (WTC), part of the National Institute of Standards and Technology Investigation of ... Specifications, Standards, and Sources 129 [...]... performance of WTC 1 and WTC 2 under design, service, and abnormal loads, and aircraft impact damage on the structural, fire protection, and ... of the WTC towers to fires with and without aircraft damage, the response of WTC 7 in fires, the performance of composite steel- trussed... 1-2B National Institute of Standards and

Ngày tải lên: 01/04/2014, 00:20

... and Copyright © TDV – Technische Datenverarbeitung Ges.m.b.H. Heinz Pircher und Partner Disclaimer Much time and effort have gone into the development and documentation of RM2000 and ... must understand the assumptions of the program and must apply engineering knowledge and skill to independently verify the results. Copyright The computer programs RM2000, GP2000 and all ... associated documentation are proprietary and copyrighted products. Ownership of the program and the documentation remain with TDV Austria. Use of the program and the documentation is restricted to...

Ngày tải lên: 06/09/2012, 15:55