Transcriptional regulation of protein complexes in yeast potx

... set of protein complexes in yeast: mining large scale protein- protein inter- action screens. Bioinformatics 2003, 19:1901-1908. 35. Manke T, Bringas R, Vingron M: Correlating protein- DNA and protein- protein ... clusters in the network linking annotated protein complexes and regulonsFigure 1 (see following page) Detailed view of the main clusters in the network linking...

Ngày tải lên: 09/08/2014, 20:20

... coexpression of a-synuclein and protein tau was synergistically toxic in yeast cells. The combined data underline the poten- tial offered by these yeast models for defining the underlying mechanisms of ... [2,37,38]. Interest- ingly, coexpression of native wt-synuclein with the A30P–EGFP fusion protein resulted in the formation of inclusions containing A30P-synuclein (Fig....

Ngày tải lên: 23/03/2014, 13:20

... oscillations seen in intact yeast cells. In the case of yeast extracts, we use flux-change plots and model analyses to establish that the oscillations are driven by on ⁄ off switching of phosphofructokinase. In ... exploit this understanding of the dynamic system to obtain an interpretation of the biochemical nature of the activating and inhibiting modes. In essence, we d...

Ngày tải lên: 30/03/2014, 16:20

... levels of glutamate. Instead, there was a corresponding trend in the increase in the transcript and protein levels of the mitochondrial 2-oxoglutarate transporters, and an increase in protein levels ... pathways in order to double mass while maintaining the same cell size distribution after cell division? In proliferating cells, with increasing growth rates, in addition to...

Ngày tải lên: 06/08/2014, 18:21

... 2004 rate-determining factor in uncoupling protein (UCP) activ- ity [16]. Thus, t he role of mitochondrial i PLA 2 activities in regulating mitochondrial function is just now beginning to be understood. ... predicted site for binding of nuclear proteins. Utilizing radiolabeled oligonucleotide dimer g50 (corres- ponding to residues 6–50 starting from the 88 kDa AUG codon) a single sh...

Ngày tải lên: 19/02/2014, 16:20

... motifs which are involved in Sm protein protein interactions. EMBO J 14, 2076–2088. 34 Se ´ raphin B (1995) Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as ... with that of Lsm1p in Lsm818p, and the Sm domain of Lsm1p was replaced with that of Lsm8p in Lsm181p. Localization of these mutant proteins GFP-tagged at the N- or C-terminus...

Ngày tải lên: 07/03/2014, 02:20

... rate of decrease in the amount of ICAD in the mutant was slower than that in DT40. Thus, in E2A )/) , in the presence of PMA/ionomycin, the time courses of alter- ations in the mRNA and protein ... Analyses of mechanisms of the resistance to PMA/ionomycin-mediated apoptosis in E2A )/) . (A) Effects of PMA/ionomycin treatment on protein levels of survivin, IAP...

Ngày tải lên: 16/03/2014, 04:20

... explain the decrease in the relative LD content per cell as a consequence of the sharing of a constant amount of LD between an increasing number of cells. From our measurements, and in agreement with published ... proportional to the change in the surface area of the cell. As membranes are typi- cally of constant thickness, any increase in membrane material results in a...

Ngày tải lên: 16/03/2014, 04:20

... p300 ⁄ cAMP response element binding protein- binding protein (CBP ⁄ p300) [26–28]. This may explain the up -regulation (alleviation of the repressing effect of GII-Sil) of the hGnRH II promoter by ... DNA protein binding Complex in this region. Although the hGII-Sil region had a similar gene-repressive effect in both cell lines, slightly different DNA protein binding pattern...

Ngày tải lên: 16/03/2014, 10:20

... was clearly higher at increasing protein concentrations, giving two DNA protein complexes of different size. This is in agreement with our previous finding on the binding of two (or four) DmdR1 ... desABCD in both S. coelicolor and S. pilosus Binding of purified DmdR1 to the desA promoter region of S. coelicolor was studied using a 511-bp PCR fragment of this region in the...

Ngày tải lên: 16/03/2014, 11:20