Atomic Force Microscopy in Cell Biology Episode 2 Part 11 ppt

... studies, 20 2 20 3 Finite element modeling, AFM interfacing, 1 82 Fluids advanced handling, AFM studies DNA digestion by DNase, 22 7 future outlook, 22 7 operation principles, 22 6 22 7 overview, 22 5 22 6 aqueous, ... modifications, 20 5 20 6 recording mode contact mode, 20 8 20 9 fast 3D single-particle tracking, 21 0 tapping mode, 20 9 21 0 resolution, 20 7 20 8 sample preparatio...

Ngày tải lên: 06/08/2014, 02:20

... crystal. 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 9 Fig. 14 Native bacteriorhodopsin assembled into an orthorhombic lattice. (A) In this crystal form (p 22 1 2 1 ) the ... native 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 1 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserted into the phos...

Ngày tải lên: 06/08/2014, 02:20

... (20 00). New insight into cellulose structure by atomic- force microscopy shows the I-alpha crystal phase at near -atomic resolution. Biophys. J. 79, 113 9– 114 5. Betzig, E., Finn, P. L., and Weiner, ... immunoglobulin and fibronectin III domains in the muscle protein titin measured by atomic force microscopy. Biophys. J. 75, 3008–3014. Rief, M., Pascual, J., Saraste, M., and G...

Ngày tải lên: 06/08/2014, 02:20

... studies, 20 3 20 4 R Recording mode photonic force microscopy contact mode, 20 8 20 9 fast 3D single-particle tracking, 21 0 tapping mode, 20 9 21 0 Resolution, PFM studies, 20 7 20 8 RL cells, adhesion force ... 21 5 cleaning, 117 118 crosslinker binding, 119 esterification, 118 large assemblies, cryo-AFM studies, 25 0 25 2 ligand binding, 119 ligand density and functiona...

Ngày tải lên: 06/08/2014, 02:20

... The AFM Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944– 1946. 3. ... in Molecular Biology, vol. 24 2: Atomic Force Microscopy: Biomedical Methods and Applications Edited by: P. C. Braga and D. Ricci © Humana Press Inc., Totowa, NJ...

Ngày tải lên: 06/08/2014, 02:20

... decorated microtubulesusing tapping mode atomic force microscopy in fluids. Eur. Biophys. J. 28 , 611 620 . 12. Lyubchenko, Yu., L., Shlyakhtenko, L. S., Harrington, R. E., Oden, P. I., and Lindsay, S. M. (1993) Atomic ... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 121 – 128 . 15. Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unger...

Ngày tải lên: 06/08/2014, 02:20

... sectioning using quantitative differential interference contrast light microscopy (20 ,21 ). Here, we present an alternative new method using atomic force microscopy (AFM) for measuring cell volume in ... is commonly used for blood cells in suspension. In contrast with spherical cells in suspension, ECs under From: Methods in Molecular Biology, vol. 24 2: Atomic Forc...

Ngày tải lên: 06/08/2014, 02:20

... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (1985) Computer-based determina- tion of size and shape in living cells. J. Microsc. 137, 28 1 29 2. 21 . Timbs, M. M. and Spring, ... cells in phosphate-buffered saline (PBS) 1 mM CaCl 2 , 3 mM KCl, 1 mM K 2 HPO 4 ; 2 mM MgCl 2 , 140 mM NaCl, 8 mM Na 2 HPO 4 ; pH 7.4 (2 × 5 min). 2. Depending upon the ep...

Ngày tải lên: 06/08/2014, 02:20

... Ultramicroscopy 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, ... (1986). Atomic force microscope. Phys. Rev. Lett. 56, 930. Charras, G. T., and Horton, M. A. (20 02 A). Determination of cellular strains by combined atomic force mic...

Ngày tải lên: 06/08/2014, 02:20

... aldehyde groups. Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both ... Plate.) Fig. 3 AFM imaging of laminin molecules in air shows submolecular structure in the laminin arms (top row). In the sequential images, a single laminin molecule in aqueo...

Ngày tải lên: 06/08/2014, 02:20