Atomic Force Microscopy in Cell Biology Episode 1 Part 1 ppt

... µg/mL and 1 mg/mL. After adsorption the AFM of Protein Complexes 217 217 16 Atomic Force Microscopy of Protein Complexes Olga I. Kiselyova and Igor V. Yaminsky 1. Introduction Scanning probe microscopy ... 72, 18 9R 19 6R. 5. Nagao, E. and Dvorak, J. A. (19 98) An integrated approach to the study of living cells by atomic force microscopy. J. Microsc. 19 1, 8 19 . 6. Radm...

Ngày tải lên: 06/08/2014, 02:20

... Wehrle-Haller, B., and Imhof, B. A. (19 98). Actin dynamics in living mammalian cells. J. Cell Sci. 11 1, 16 49 16 58. Barbee, K. A., Mundel, T., Lal, R., and Davies, P. F. (19 95). Subcellular distribution of ... Struct. Biol. 12 0, 18 0 19 1. Lehenkari, P. P., Charras, G. T., and Horton, M. A. (19 99). New technologies in scanning probe microscopy for the understanding of molecu...

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... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 12 1 12 8. 15 . Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unger, E., and Jovin, T. M. (19 95) Scanning force microscopy ... 257, 19 00 19 05. 13 . Zasadzinski, J. A. N., and Hansma, P. K. (19 90) Scanning tunneling microscopy and atomic force microscopy of biological surfaces. Ann...

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... of the surrounding protein structure (Fig. 11 A; compare to Fig. 10 B). Increasing the force to approximately 15 0 pN further pushed the EF loop away (Fig. 11 B), whereas at about 18 0 pN the conformational change ... native 13 . Atomic Force Microscopy and Spectroscopy of Membrane Proteins 2 71 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserte...

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... (2000). New insight into cellulose structure by atomic- force microscopy shows the I-alpha crystal phase at near -atomic resolution. Biophys. J. 79, 11 39– 11 45. Betzig, E., Finn, P. L., and Weiner, J. ... 14 3 14 6. Rief, M., Gautel, M., Schemmel, A., and Gaub, H. E. (19 98). The mechanical stability of immunoglobulin and fibronectin III domains in the muscle protein titin measure...

Ngày tải lên: 06/08/2014, 02:20

... overview, 14 7 14 8 tips cleaning, 11 7 11 8 crosslinker binding, 11 9 esterification, 11 8 ligand binding, 11 9 ligand density and funtionality, 11 9 12 0 modifications, 17 7 17 8 probe surfaces, 12 0 12 1 shape, ... functionality, 11 9 12 0 probe surfaces, 12 0 12 1 applications, 11 6 dynamic force microscopy bond lifetime, 12 4 kinetic rates, energies, binding pocket, 12 5 u...

Ngày tải lên: 06/08/2014, 02:20

... studies, 215 cleaning, 11 7 11 8 crosslinker binding, 11 9 esterification, 11 8 large assemblies, cryo-AFM studies, 250–252 ligand binding, 11 9 ligand density and functionality, 11 9 12 0 probe surfaces, 12 0 12 1 Surfaces SFM ... studies, 3 71 374 Spectrin, AFM studies, 313 Staphylococcal α-hemolysin, AFM studies, 270 Stereocilia, 14 4 14 6 STM, see Scanning tunneling microscopy S...

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... Small Resolution 10 0 nm 5 nm 0.7 nm 0 .1 1. 0 nm x, y, z n/a n/a n/a 0. 01 nm Magnification 1x–2 × 10 3 x 10 x 10 6 x 9x 10 5 x5 × 10 2 x 10 8 x Necessary sample Low Critical point Critical point Low preparation ... (19 94) An atomic force microscope for cytological and histological investigations. J. Microsc. 17 6, 12 1 13 1. 12 . Ushiki, T., Hitomi, J., Ogura, S., Umemoto,...

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... determined for each conjugation. A suggested range of dilutions is 1: 10 to 1: 100. 3. Wash cover slips in PBS (4 × 5 min) at 4°C and then fix cells for 15 min in 0.25% glutaraldehyde in 0 .1 M sodium ... (19 98) Atomic force microscopy on living cells: aldosterone-induced localized cell swelling. Kidney Blood Press. Res. 21, 256–258. 12 . Cines, D. B., Pollak, E. S. , Buc...

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... Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both cases, 10 ... Geerts, H. (19 91) . Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with nanovid microscopy. J. Cell Biol. 11 2, 11...

Ngày tải lên: 06/08/2014, 02:20