Enzymes and Recombinant enzyme technique docx

... alfa. • Glucocerebrosidase. Application of Enzyme Application : Enzyme used: Foodprocessing Amylase from fungi and plants Baby food Tripsin Topic: Enzymes and Recombinant enzyme technique. Student: Tran ... 0802 Recombinant enzyme technique (ReE) • Difinition: ReE are proteins which are produced by recombinant DNA technique. • History of recombinant DNA...

Ngày tải lên: 02/08/2014, 08:21

... Arabidopsis and potato enzymes [4]. The efficiency is lower for the oilseed rape enzyme compared to the Arabi- dopsis enzyme, AtsEH1, expressed in P. pastoris [26]. The Arabidopsis enzyme had a ... antibodies raised to recombinant sEH1 from A. thaliana (AtsEH1). The samples analysed were purified recombin- ant AtsEH1 enzyme (lane 1), purified recombinant BNSEH1 enzyme (lane 2)...

Ngày tải lên: 31/03/2014, 08:20

... chromatography and gel filtration were identical to those observed for the native enzyme. The purification of recombinant AADH is illustrated in Fig. 2 and summarized in Table 1. The recombinant enzyme ... active recombinant AADH that requires the cooperation of TTQ biogenesis genes from the mau gene cluster. By performing detailed kinetic studies of both AADH enzymes, we show tha...

Ngày tải lên: 19/02/2014, 07:20

... l M ¯uorescein Tat peptide and analyzed by FACS. RESULTS Uptake and cellular localization of ¯uorescently labeled Tat peptides Cellular uptake of the full-length Tat p rotein fused to GFP and/ or GST involves ... temperature, and washed again with NaCl/P i before being processed in Vectashield TM mount- ing solution (Vector Laboratories Inc., Burlingame, CA, USA). Internalization and...

Ngày tải lên: 21/02/2014, 03:20

... (glycoside hydrolase fam- ilies 18 and 19) and chitosanases (glycoside hydrolase families 46, 75 and 80) [11]. These enzymes differ with respect to their preference for A- and D-units on each side of ... a DP of 3.04 and a F A of 0.44, and was concluded to consist mainly of DDA and DAA, in addition to minor amounts of ADA. The tetramer fraction showed a DP of 4.1 and a F A o...

Ngày tải lên: 07/03/2014, 16:20

... class II ALDO, iPGAM, and the PPi-dependent enzymes PPi-PFK and PPDK, are not found in human cells (Table 1). Gene cloning, overexpression and purification of recombinant glycolytic enzymes The genes ... galactose. Amoebal HK isoenzymes are strongly inhibited by AMP and ADP, but glucose 6-phosphate (Glc6P), the potent modulator of some mammalian HK enzymes [16], is a weak inhib...

Ngày tải lên: 07/03/2014, 17:20

... decrease (Fig. 2B and Table 2). We expressed donor and accep- tor proteins in the pairs pVHL30-cerulean and elon- gin C-citrine, cerulean-pVHL30 and citrine-elongin C, and cerulean-pVHL30 and elongin ... combinations: cerulean-elongin B and citrine- elongin C; cerulean-elongin B and elongin C-citrine; elongin B-cerulean and elongin C-citrine; and elon- gin B-cerulean and...

Ngày tải lên: 16/03/2014, 05:20

... as the standards and their elution volumes, V e , were deter- mined. The standard curve was plotted with the logarithm of molecular mass against V e /V 0 of the standard protein. Peptidase and ATPase ... m M NaCl, 10 m M MgCl 2 and 50 m M Tris/HCl, pH 8.0, for 20 min with plasmid DNA (500 ng) at the indicated temperatures. Analysis used standard 0.8% agarose gels, and DNA bands were v...

Ngày tải lên: 16/03/2014, 16:20

... expression and properties of the recombinant enzyme Y. T. Pan 1 , J. D. Carroll 2 and A. D. Elbein 1 1 Department of Biochemistry and Molecular Biology and 2 Department of Microbiology and Immunology, ... the cloning and expression of the M. tuberculosis tps gene in E.coli,andthe production of active TPS in these cells. The properties of the recombinant enzyme have been de...

Ngày tải lên: 17/03/2014, 10:20

... ascorbate oxidase and m ammalian plasma protein ce ruloplasmin are other enzymes that were classified in the same family and these have been studied extensively by biochemical and structural characterization ... three-step procedure and allowed to study the physico-chemical properties of the recombinant enzyme for comparison with native laccase. A ll the m ain characteristics of...

Ngày tải lên: 17/03/2014, 11:20