MOLECULAR CLONING OF CHITINASE 33 (CHIT33) GENE FROM TRICHODERMA ATROVIRIDE doc

... Microbiol., 137, 2163-2169. Brazilian Journal of Microbiology (2008) 39: 433- 437 ISSN 1517-8382 433 MOLECULAR CLONING OF CHITINASE 33 (CHIT33) GENE FROM TRICHODERMA ATROVIRIDE Matroudi S.; Zamani M.R.; ... identification of T. atroviride as a high producer of chitinolytic enzymes and cloning and partial characterization of its endochitinase gene (chit3...

Ngày tải lên: 29/07/2014, 23:20

... hormonal regulation of the rat matrix (MGP) gene in chondrogenesis and osteogenesis. J. Cell. Biochem. 46, 351–365. 35. Shanahan, C.M., Weissberg, P.L. & Metcalfe, J.C. (1993) Isola- tion of gene markers of ... fragments from the xMGP gene promoter almost completely prevented the formation of the DNA- protein complexes (Fig. 7). In contrast, addition of an excess of DN...

Ngày tải lên: 08/03/2014, 10:20

... transfer of the c-phosphate group from ATP to AMP, releasing two molecules of ADP. This study describes cloning and functional characterization of the gene encoding AK2 from a genomiclibrary of Leishmania ... The analysis of the nucleotide sequence revealed the presence of a 690-bp long ORF that encoded a protein of 230 amino acids, with an estimated molecular mass of...

Ngày tải lên: 21/02/2014, 00:20

... regulation system of tyrosinase in cephalopods, as in the case of arthropods [14–17]. Molecular cloning of ST94 cDNA The cDNA cloning of tyrosinase ST94 was carried out by degenerate RT-PCR and ... Uraga, Y. & Ichisima, E. (1995) Molecular cloning and nucleotide sequence of the protyrosinase gene, melO ,from Aspergillus oryzae and expression of the gene in yeast...

Ngày tải lên: 17/03/2014, 10:20

... were the gift of Dr Nagakura of Kobe Women’s College of Phar- macy, and (S)-reticuline was a gift from Dr Facchini of the University of Calgary. The other alkaloids were generous gifts from Mitsui ... their generous gifts of the alkaloids. We also thank Ms. L. Huang for her technical assistance with preparing columbamine. We thank Dr W. Frommer of the University of Tuebing...

Ngày tải lên: 17/03/2014, 10:20

... suppressalis EcR-A Fig. 2. Alignment of the primary sequences of (A) A ⁄ B regions of EcRs, (B) E regions of EcRs, (C) A ⁄ B regions of USPs and RXRs, and (D) E regions of USPs and RXRs. Alignments were ... identity of LaRXR to the RXRa of Homo sapiens is relatively high (63%). We also compared A ⁄ B, C, D and E Table 2. Identities of amino acid sequences of EcR-A isoforms...

Ngày tải lên: 30/03/2014, 04:20

... Chitosanase gene cloning A single fragment of 270 bp was amplified using degenerate primers DFP and DRP from the genomic DNA of Penicillium sp. D-1. The deduced amino acid sequence of this ... Characterization of two chitinase genes and one chitosanase gene encoded by chlorella virus PBCV-1. Virology 263:376-387 Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molec...

Ngày tải lên: 21/06/2014, 19:20

... Homology model of HYD Js . (A) Docking of D-p-HPH to the HYD Js active site. (B) The SGLs of HYD Bp are shown as magenta lines and those of HYD Js are shown in green. The transition state of D-p-HPH ... understanding of the roles of each of these residues will enable manipu- lation of these SGLs by rational design or molecular evolution methods to obtain the desired cataly...

Ngày tải lên: 18/02/2014, 08:20

... between 71% and 95% of the amount Fig. 3. SDS/PAGE of extracts of recombinant E. coli containing NoxA-1, NoxB-1 or NoxC from A. fulgidus. M, Calibration proteins. The molecular mass of the calibration ... adjacent genes of NoxB-1 do not reveal any information concerning its function. On the other hand, the gene encoding NoxB-2 of A. fulgidus, which is 98.9% identical to NoxB...

Ngày tải lên: 08/03/2014, 02:21

... transgenic plants Ó FEBS 2002 Cloning of manganese lipoxygenase (Eur. J. Biochem. 269) 2695 Cloning of the manganese lipoxygenase gene reveals homology with the lipoxygenase gene family Lena Ho¨ rnsten 1 , ... Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxyg- enase gene. cDNA analysis showed that the gene spanned 2.6 kb and conta...

Ngày tải lên: 18/03/2014, 01:20