Handbook of Microbiological Media, Fourth Edition part 194 doc

... 40.0g Preparation of Buffer Solution: Add 40.0g of Na 2 HPO 4 to 300.0mL of distilled/deionized water. Mix thoroughly. Add 60.0g of KH 2 PO 4 . Mix thoroughly. Adjust pH to 6.0. Preparation of Medium: ... 50–55°C. Preparation of Medium: Aseptically combine 500.0mL of solu- tion A and 500.0mL of solution B. Pour into sterile Petri dishes or dis- tribute into sterile tubes. Us...

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... Medium, 1943 ,1944 YGC Medium with Cysteine, 1944 YCJC Medium with Glutamic Acid. 1944 YGCB Salt Medium, 1944 YGCP Medium. 1944 YGI.M. 1945 YGI.M with Chalk, 1945 YGI.PB Medium, 1945 YGI.PB ... Broth, 1936 YM Medium. 1946 YM-IE Broth. 1946 YM5 with 10% Sorbitol, 1947 YMAAgar. 1947 YMA Medium, 1947 YMF Agar. 1947 YMI Broth. 1947 YNA Medium, 1948 YNG Medium....

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... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... 1.0g Preparation of FeCl 3 Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Bristol's Solution: A...

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... pres- sure–121°C. Preparation of Medium: To 916.0mL of sterile solution A add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Use: For the cultivation of anaerobic ... cultivation of a variety of anaerobic microorganisms, especially Clostridium species. Anaerobic Agar Composition per liter: Pancreatic digest of c...

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... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of...

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... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... and anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O sol...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is...

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... pressure–121°C. Add 0.25mL of sterile NaHCO 3 solution and 0.25mL of sterile sodium thioglycolate solu- tion for each 10.0mL of sterile basal medium. Use: For the cultivation and maintenance of Clostridium ... plate culture of other uri- nary organisms, while preventing the swarming of Proteus spp. One chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the specific...

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... 15.0mL Preparation of Part B: Prepare a 1M solution of K 2 CO 3 and filter sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15.0mL of ... K 2 CO 3 solution and 15.0mL of sterile etha- nol. Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL of sterile, cooled Part A. Adjust pH to 7.0...

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