Handbook of Microbiological Media, Fourth Edition part 194 doc
Handbook of Microbiological Media, Fourth Edition part 194 doc
... 40.0g
Preparation of Buffer Solution: Add 40.0g of Na
2
HPO
4
to
300.0mL of distilled/deionized water. Mix thoroughly. Add 60.0g of
KH
2
PO
4
. Mix thoroughly. Adjust pH to 6.0.
Preparation of Medium: ... 50–55°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A and 500.0mL of solution B. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Us...
Handbook of Microbiological Media, Fourth Edition part 204 doc
... Medium, 1943 ,1944
YGC Medium with Cysteine, 1944
YCJC Medium with Glutamic Acid. 1944
YGCB Salt Medium, 1944
YGCP Medium. 1944
YGI.M. 1945
YGI.M with Chalk, 1945
YGI.PB Medium, 1945
YGI.PB ... Broth, 1936
YM Medium. 1946
YM-IE Broth. 1946
YM5 with
10%
Sorbitol, 1947
YMAAgar. 1947
YMA Medium, 1947
YMF Agar. 1947
YMI Broth. 1947
YNA Medium, 1948
YNG Medium....
Handbook of Microbiological Media, Fourth Edition part 8 docx
... 10.0mL of CaCl
2
solution, 10.0mL of MgSO
4
·7H
2
O solution,
10.0mL of NaNO
3
solution, 10.0mL of K
2
HPO
4
solution, 10.0mL of
KH
2
PO
4
solution, and 10.0mL of NaCl solution to distilled/deionized
water ... 1.0g
Preparation of FeCl
3
Solution: Add FeCl
3
to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Bristol's Solution: A...
Handbook of Microbiological Media, Fourth Edition part 11 doc
... pres-
sure–121°C.
Preparation of Medium: To 916.0mL of sterile solution A add
70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL
of sterile solution D. Mix thoroughly.
Use: For the cultivation of anaerobic ... cultivation of a variety of anaerobic microorganisms,
especially Clostridium species.
Anaerobic Agar
Composition per liter:
Pancreatic digest of c...
Handbook of Microbiological Media, Fourth Edition part 13 doc
... to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. ... to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of...
Handbook of Microbiological Media, Fourth Edition part 15 docx
... determination of phenol coefficients of disinfectant prod-
ucts containing cationic surface-active materials. Use according to
Official Methods of Analysis of the Association of Official Analytical
Chemists ... inoculation of cultures, add 0.25mL of
sterile Na
2
S·9H
2
O solution to each tube containing 9.75mL of sterile basal
medium.
Use: For the cultivation and maintenance...
Handbook of Microbiological Media, Fourth Edition part 18 docx
... and
anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of
sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10.0mL of sterile Na
2
S·9H
2
O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of
sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10.0mL of sterile Na
2
S·9H
2
O sol...
Handbook of Microbiological Media, Fourth Edition part 34 docx
... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is partially lysed due ... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is...
Handbook of Microbiological Media, Fourth Edition part 40 doc
... pressure–121°C. Add 0.25mL of
sterile NaHCO
3
solution and 0.25mL of sterile sodium thioglycolate solu-
tion for each 10.0mL of sterile basal medium.
Use: For the cultivation and maintenance of Clostridium ... plate culture of other uri-
nary organisms, while preventing the swarming of Proteus spp. One
chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the
specific...
Handbook of Microbiological Media, Fourth Edition part 42 docx
... 15.0mL
Preparation of Part B: Prepare a 1M solution of K
2
CO
3
and filter
sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically
combine 20.0mL of sterile K
2
CO
3
solution and 15.0mL of ... K
2
CO
3
solution and 15.0mL of sterile etha-
nol.
Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL
of sterile, cooled Part A. Adjust pH to 7.0...
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