clinical handbook of couple therapy fourth edition download

... of Medium: Aseptically combine 900.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... Aseptically and anaerobi- cally add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile sucrose solution, 10.0mL of sterile Na 2 S·9H 2 O solution, and 10.0mL of sterile Wolfe’s vitamin solution. ... Immediately prior to inoculation, reduce medium by adding 1.0mL of titanium citrate solution per liter of medium. Use: For the cultivation of Amphibacillus xylanus. Alkaline Yeast Extract Malt Medium

Ngày tải lên: 03/07/2014, 18:20

... detection of antibiotics in milk, and determination of the antimicrobial effectiveness of antibiotics. Antibiotic Medium 1 with Tetracycline Composition per liter: Agar 15.0g Pancreatic digest of gelatin ... maintenance of Salmonella choleraesuis. Antibiotic Medium 1 with Tetracycline, Streptomycin, and Chloramphenicol Composition per liter: Agar 15.0g Pancreatic digest of gelatin 6.0g Pancreatic digest of ... dilution assay of pen- icillins and other antibiotics. Used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus. For the cultivation and main- tenance of Bacillus

Ngày tải lên: 03/07/2014, 18:20

... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance of Archaeoglobus profun- dus. ... enumeration of bacteria, especially heter- ofermentative lactobacilli, from meat and other foods. For the cultiva- tion of streptococci. APT Broth Composition per liter: Pancreatic digest of casein

Ngày tải lên: 03/07/2014, 18:20

... to 45°–50°C. Preparation of Medium: To 1.0L of sterile Eagle’s basal medium, aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of fetal calf serum. Mix thoroughly. Pour into ... and maintenance of Aureobacterium flave- scens. Arthrobacter YCWD Composition per liter: Pancreatic digest of casein 10.0g Yeast extract 1.0g pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components ... sure–121°C. Cool to 50°C. Aseptically add 20.0mL of sterile modified Hutner’s basal salts solution, 20.0mL of sterile phosphate supplement solution, and 1.0mL of sterile artificial organic lake vitamin

Ngày tải lên: 03/07/2014, 18:20

... Preparation of NAD Solution: Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700.0mL of PPLO broth ... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nutrient Agar: ... imize the incorporation of air into the medium. Pour into sterile Petri dishes. Use: For the cultivation and enumeration of hydrocarbon-utilizing bacteria by direct plating of water and sediment

Ngày tải lên: 03/07/2014, 18:20

... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... Preparation of Medium: Aseptically combine 1.0L of sterile solu- tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of

Ngày tải lên: 03/07/2014, 18:20

... Aseptically add 2.5mL of sterile hemin solution, 2.5mL of sterile gentamicin solution, and 1.0mL of sterile vitamin K 1 solution. Use: For the selection and presumptive identification of the Bacteri- ... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group

Ngày tải lên: 03/07/2014, 18:20

... isolation of Gram-negative enteric bacilli from clinical and nonclinical specimens. For the isolation and identifi- cation of microorganisms from fecal specimens. For the isolation of Salmonella, ... cultivation, and differentiation of Gram-negative enteric bacilli from clinical and nonclinical specimens. For the isolation, cultivation, and identification of microorganisms from fecal specimens. ... Blue B to 200.0mL of distilled/deionized water. Mix thoroughly. Filter ster- ilize. Warm to 50°C. Preparation of Medium: Aseptically combine 1.0L of sterile basal medium with 50.0g of sterile fat

Ngày tải lên: 03/07/2014, 18:20

... cultivation of enteric bacilli from food and in treated drinking water. Bean Agar Composition per liter: Dry white beans 250.0g Agar 20.0g Preparation of Medium: Soak beans in 500.0mL of distilled/de- ... dishes or leave in tubes. Use: For the cultivation and maintenance of a wide variety of micro- organisms. Recommended for the culture of microorganisms from milk and water. Beef Extract Agar (ATCC ... 15 psi pressure–121°C. Use: For the cultivation and maintenance of a wide variety of micro- organisms. Recommended for the culture of microorganisms from milk and water. Beef Extract Broth (ATCC

Ngày tải lên: 03/07/2014, 18:20

... Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution: Composition per 10.0mL: Sodium nitrofurantoin 0.01g Preparation of Sodium Nitrofurantoin Solution: Add sodium nitrofurantoin to distilled/deionized water and bring volume ... digest of casein 20.0g Yeast extract 10.0g Peptone 10.0g Tomato juice 333.0mL Tween™ 80 2.0mL pH 6.8 ± 0.2 at 25°C Preparation of Medium: Combine 333.0mL of tomato juice with 666.0mL of distilled/deionized

Ngày tải lên: 03/07/2014, 18:20

... Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster- ile solution B. ... Petri dishes or leave in tubes. Use: For the cultivation of a variety of microorganisms. For the prep- aration of blood agar by the addition of sterile blood. Blood Agar Base with Blood Composition ... 50.0mL of sterile, defibrinated sheep blood. Mix thoroughly and pour into sterile Petri dishes. Use: For the isolation and growth of a wide variety of microorgan- isms. For the detection of the

Ngày tải lên: 03/07/2014, 18:20

... 0.1g Riboflavin solution 5.0mL Riboflavin Solution: Composition per 10.0mL: Riboflavin 2.0mg Preparation of Riboflavin Solution: Add riboflavin to 10.0mL of distilled/deionized water. Mix thoroughly. ... identification of clinically important Candida species. The green color of Candida albicans and Candida dubliniensis is caused by the same chromogenic reaction as the dark blue color of Candida tropicalis. ... growth of a wide variety of competitor organ- isms and MSSA. Also included are compounds to suppress the expres- sion of phosphatase activity in other staphylococci, thus ensuring a high level of

Ngày tải lên: 03/07/2014, 18:20

... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution, 0.1mL of sterile nystatin solution, and 0.1mL of sterile tripeptide solution. ... Preparation of Medium: To 950.0mL of cooled, sterile anaerobic agar base, aseptically add 10.0mg of vitamin K 1 and 50.0mL of sterile, de- fibrinated sheep blood. Use: For the isolation of anaerobes.

Ngày tải lên: 03/07/2014, 18:20

... Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... Preparation of Medium: To 950.0mL of cooled, sterile basal me- dium, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 4.0mL of sterile cefopera- ... Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 4.0mL of sterile cefoperazone

Ngày tải lên: 03/07/2014, 18:20

... Polymyxin B 2500U Preparation of Antibiotic Supplement: Add components to 10.0mL of distilled/deionized water. Filter sterilize. Preparation of Medium: Prepare 890.0mL of Brucella agar base. Sterilize ... sure–121°C. Cool to 45°–50°C. Preparation of Medium: To 890.0mL of cooled, sterile Columbia agar base, aseptically add 100.0mL of sheep blood or 50.0–70.0mL of laked horse blood. Laked blood is prepared ... mation and inhalation. Preparation of Medium: To 940.0mL of cooled, sterile Brucella agar base, aseptically add 50.0mL of defibrinated sheep or horse blood and 10.0mL of sterile antibiotic supplement.

Ngày tải lên: 03/07/2014, 18:20

... a holding medium—of clinical speci- mens during collection or shipment. Caryophanon latum Medium Composition per liter: Papaic digest of soybean meal 2.0g Pancreatic digest of casein 2.0g Yeast ... the cultivation of Acetobacter pasteurianus. Carrot Decoction Agar Composition per liter: Carrots 100.0g Agar 15.0g Preparation of Medium: Peel and slice carrots. Add to 1.0L of dis- tilled/deionized ... solution) 200.0mL Preparation of Resazurin Solution: Add resazurin to 200.0mL of ethanol. Mix thoroughly. Bring volume to 380.0mL with distilled/de- ionized water. Preparation of Medium: Add components,

Ngày tải lên: 03/07/2014, 18:20

... 10.0mL of sterile CaCl 2 ·2H 2 O solution, 10.0mL of sterile MgSO 4 ·7H 2 O solution, 10.0mL of sterile sodium ac- etate solution, 2.0mL of sterile yeast extract solution, and 1.0mL of sterile ... combine 500.0mL of sterile Castenholz salts, 2X, 100.0mL of sterile 1% TYE, and 400.0mL of sterile distilled/deionized water. Adjust pH to 7.6. Use: For the cultivation and maintenance of Thermonema ... combine 500.0mL of sterile Castenholz salts, 2X, 100.0mL of sterile 2% TYE, and 400.0mL of sterile distilled/deionized water. Adjust pH to 7.6. Use: For the cultivation and maintenance of Thermus

Ngày tải lên: 03/07/2014, 18:20

... 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Prepare 975.0mL of basal medium. Heat ... 50.0mL of ster- ile cellobiose solution, 10.0mL of sterile NaHCO 3 solution, and 10.0mL of sterile L-cysteine·HCl solution. Mix thoroughly. Use: For the cultivation and maintenance of Cellulomonas ... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled,

Ngày tải lên: 03/07/2014, 18:20

... earlier editions. Part I of this edition, a Handbookmainstay, coversimportant currentprofessionaland scientificissues,but manyofthe topicsin this edition arenew and reflect importantemerging professional ... xi Preface This edition of theHandbook of Counseling Psychology,like all three prior editions, has three primary objectives: (1) to provide a scholarly review of important areas of counseling psychology ... have completed this edition of theHandbookwithout them. Finally, we thank each other. Although a bit out of the ordinary for an acknowledgment, we’ve had, and continue to have, a heck of a professional run...

Ngày tải lên: 15/03/2014, 04:20

... high levels of amino acids. It contains total nitrogen of 11.9% and NaCl of 1.4%. Liver Digest Neutralized A papaic digest of liver that contains total nitrogen of 11.0% and NaCl of 1.6%. Mycological ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of casein and peptic digest of ... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids....

Ngày tải lên: 03/07/2014, 18:20