clinical handbook of couple therapy fourth edition pdf download

... detection of antibiotics in milk, and determination of the antimicrobial effectiveness of antibiotics. Antibiotic Medium 1 with Tetracycline Composition per liter: Agar 15.0g Pancreatic digest of gelatin ... maintenance of Salmonella choleraesuis. Antibiotic Medium 1 with Tetracycline, Streptomycin, and Chloramphenicol Composition per liter: Agar 15.0g Pancreatic digest of gelatin 6.0g Pancreatic digest of ... dilution assay of pen- icillins and other antibiotics. Used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus. For the cultivation and main- tenance of Bacillus

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... Polymyxin B 2500U Preparation of Antibiotic Supplement: Add components to 10.0mL of distilled/deionized water. Filter sterilize. Preparation of Medium: Prepare 890.0mL of Brucella agar base. Sterilize ... sure–121°C. Cool to 45°–50°C. Preparation of Medium: To 890.0mL of cooled, sterile Columbia agar base, aseptically add 100.0mL of sheep blood or 50.0–70.0mL of laked horse blood. Laked blood is prepared ... mation and inhalation. Preparation of Medium: To 940.0mL of cooled, sterile Brucella agar base, aseptically add 50.0mL of defibrinated sheep or horse blood and 10.0mL of sterile antibiotic supplement.

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... Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to 950.0mL of distilled/deionized ... Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to 950.0mL of distilled/deionized ... Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 99.0mL of absolute ethanol. Mix thoroughly. Hemin Solution: Composition per 100.0mL: Hemin 1.0g NaOH (1N solution) 20.0mL Preparation of Hemin

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... aseptically and anaerobi- cally add 1.0mL of filter-sterilized methanol and 1.0-2.0mL of methane- thiol gas to each liter of medium. Addition of 10-20mg of sodium dithionite per liter (e.g., from ... Use: For the isolation of pathogenic Neisseria species. For antimicro- bial susceptibility testing of a variety of bacterial species. For the cul- tivation and maintenance of Moraxella osloensis ... Preparation of Selective Supplement Solution B: Add 0.2g of 2,3,5,triphenyltetrazolium chloride to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of

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... Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile solution A, 3.0mL of sterile solution B, and 1.0mL of sterile trace elements so- lution SL-4. Mix thoroughly. Aseptically ... Preparation of Page’s Amoeba Saline: Add components to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium: Add the glucose and ox liver digest to 1.0L of Page’s ... maintenance of Bordetella bronchisep- tica. Neomycin Assay Agar See: Antibiotic Medium 11 Neomycin Blood Agar Composition per liter: Pancreatic digest of casein 14.5g Agar 14.0g Papaic digest of soybean

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... Preparation of VFA Solution: Add volatile fatty acids to 98.0mL of KOH solution. Mix thoroughly. Filter sterilize. Store at 4°C. Preparation of Medium: Combine 20.0mL of NaHCO 3 solution, 20.0mL of rabbit ... Preparation of VFA Solution: Add volatile fatty acids to 98.0mL of NaOH solution. Mix thoroughly. Filter sterilize. Store at 4°C. Preparation of Medium: Open the flask containing 94.0mL of cooled ... Preparation of Chelated Metals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Adjust pH of 1.0L of seawater

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... Aseptically add 60.0mL of sterile se- rum-glucose solution, 12.5mL of sterile bacitracin solution, 10.0mL of sterile cycloheximide solution, 2.0mL of sterile nystatin solution, 1.0mL of sterile polymyxin ... 2.0mL Preparation of Nalidixic Acid Solution: Add nalidixic acid to 2.0mL of NaOH solution. Mix thoroughly. Immediately before use, add 1.0mL of this stock solution to 9.0mL of distilled/deionized ... and anaerobically add 20.0mL of sterile trimethylamine·HCl so- lution, 20.0mL of sterile Na 2 CO 3 solution, and 10.0mL of sterile Na 2 S·9H 2 O solution per 950.0mL of medium. Check that final

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... Use: For the selective isolation of Vibrio cholerae and Vibrio para- haemolyticus from a variety of clinical and nonclinical specimens. For the cultivation of enteropathogenic vibrios causing ... Cool to 50°–55°C. Preparation of Medium: Aseptically combine 50.0mL of sterile solution A, 200.0mL of sterile solution B, 750.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. ... Cool to 50°C. Asepti- cally add 4.0mL of the sterile glucose solution, 2.0mL of the sterile thymine solution, 2.0mL of the sterile alanine solution, and 1.6mL of the sterile streptomycin sulfate

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... vitamins 100.0mL Preparation of Special 107 Vitamin Mix: Aseptically combine 1.2mL of sterile solution 1, 0.4mL of sterile solution 2, 0.4mL of sterile solution 3, and 100.0mL of sterile solution 4 ... agar, aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se- rum, 2.0mL of sterile penicillin solution, 3.0mL of sterile MES buffer solution, and 0.2mL of sterile urea solution. ... pressure–121°C. Asep- tically add 100.0mL of sterile, heat-inactivated bovine serum, 50.0mL of sterile glucose solution, 100.0mL of sterile special 107 vitamin mix, and 50.0mL of sterile buffer solution. Mix

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... 3 100.0mL Solution 4 20.2mL Preparation of Selective Reagent: Combine 100.0mL of solution 1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu- tion 4. Mix thoroughly. Gently heat ... 3 100.0mL Solution 4 20.2mL Preparation of Selective Reagent: Combine 100.0mL of solution 1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu- tion 4. Mix thoroughly. Gently heat ... 3 100.0mL Solution 4 20.2mL Preparation of Selective Reagent: Combine 100.0mL of solution 1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu- tion 4. Mix thoroughly. Gently heat

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... hydrolysis of the agar. An additional 5.0g of agar can be used to make a firmer agar. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and enumeration of yeasts. The low pH of ... cultivation of Y-1 mouse adrenal tissue culture cells used for the detection of heat-labile toxin (LT) produced by enterotoxigenic strains of Escherichia coli. LT causes the conversion of elongated ... cultivation of Stenotrophomonas maltophilia. Xanthomonas Medium Composition per liter: Pancreatic digest of gelatin 10.0g Sucrose 10.0g Beef extract 6.0g pH 6.8 ± 0.2 at 25°C Preparation of Medium:

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... thoroughly. Preparation of Milk: Autoclave 500.0mL of milk for 20 min at 15 psi pressure–115°C. Preparation of Medium: Combine 500.0mL of sterile egg yolk and 500.0mL of sterile milk. Mix thoroughly. ... temperature. Preparation of YI Broth: Aseptically add 10.0mL of 10X glucose buffer stock to 78.0mL of cooled YI base stock. Adjust osmolarity with NaCl to 380.0milliosmols/kg. Preparation of Medium: Aseptically ... 380.0milliosmols/kg. Preparation of Medium: Aseptically add 2.0mL of vitamin mix- ture 18 and 10.0mL of heat-inactivated bovine serum to 88.0mL of YI broth. Distribute in 13.0mL aliquots to 16 x 125mm

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... Association of Textile Chemists and Colorists Bacteriostasis Agar. 16. 670 American Association of Textile Chemists and Colorists Bacteriostasis Broth, 670 American Association of Textile ... 109 Anaerocellum Medium, 110 Anaerocellum ihermaphilum, 110, 111 Anaerofilum agile. 111 Anaerofilum Medium, 111 Anaerofilum pentosovorans. 111 Anaerolinea Medium, 111 Anaerolinea Medium ... brasi/ense, 1602 AzospiriUum halopraeferens, 1672 AzospiriUum lipoferum. 165.235. 1602 AzospiriUum lipoferum Agar Medium, 165 AzospiriUum lipoferum Medium. 165 AzospiriUum Medium, 165 AzospiriUum

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... for the standard methods examination of water and food. Sources of Media The Handbook of Microbiological Media includes the media produced by major suppliers of dehydrated media, including Oxoid ... occur. Some of these have been developed based upon molecular biology determinations of specific genes that are useful for the differentiation of bacterial taxa. Below is a list of some commonly ... Preparation of Media pH Buffers Maintaining the pH of media usually is accomplished by the inclusion of suitable buffers. Because microorganisms grow optimally only within certain limits of a pH

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... tubes. Use: For the maintenance of cultures of Escherichia coli and Staphy- lococcus aureus. For the detection of antibacterial activity of fabrics. Test cultures of Escherichia coli or Staphylococcus ... the resistance of textiles to fungi that cause mildew and rot. Also used to test the effectiveness of fungicides used on tex- tiles for preventing the growth of fungi. Cultures of Chaetomium glo- ... Aspergillus niger are inoculated onto the plate and a sample of fabric is placed on top. Lack of growth of these fungi on the textile is indicative of resistance to mildew. Abeyta-Hunt Bark Agar Composition

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... anaerobically add 0.1mL of sterile glucose, 0.1mL of sterile L-cysteine·HCl·H 2 O solution, and 0.1mL of sterile Na 2 S·9H 2 O solution to each tube. Mix thoroughly. Use: For the cultivation of Acetitomaculum ... autoclav- ing, the pH of the medium will be 7.2. Prior to inoculation of cultures, aseptically and anaerobically add 0.1mL of sterile reducing agent solu- tion and 0.5mL of sterile cellobiose ... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Medium: Add components, except

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... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized ... Preparation of FeCl 3 Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Bristol's Solution: Add 10.0mL of NaNO 3 solu- tion, 10.0mL of ... psi pressure–121°C. Cool to 50°–55°C. Preparation of Medium: Aseptically combine 500.0mL of solu- tion A, 1.0mL of solution B, and 500.0mL of solution C. Mix thor- oughly. Pour into sterile

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... of Medium: Aseptically combine 900.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... Aseptically and anaerobi- cally add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile sucrose solution, 10.0mL of sterile Na 2 S·9H 2 O solution, and 10.0mL of sterile Wolfe’s vitamin solution. ... Immediately prior to inoculation, reduce medium by adding 1.0mL of titanium citrate solution per liter of medium. Use: For the cultivation of Amphibacillus xylanus. Alkaline Yeast Extract Malt Medium

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... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ... Preparation of Medium: Saturate cooled solution A under 100% CO 2 at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add 860.0mL of solution B, 5.0mL of solution C, 5.0mL of solution ... cups: Sucrose 0.5 cup Preparation of Sweetened Water: Bring 2.0 cups of tap water to boiling. Add sucrose. Mix thoroughly until dissolved. Cool to 4°C. Preparation of Medium: Blanch and skin almonds.

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... Preparation of Medium: To 916.0mL of sterile solution A add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Use: For the cultivation of anaerobic ... cultivation of a variety of anaerobic microorganisms, especially Clostridium species. Anaerobic Agar Composition per liter: Pancreatic digest of casein 17.5g Agar 15.0g Glucose 10.0g Papaic digest of ... Preparation of L-Cystine Solution: Add L-cystine to 5.0mL of NaOH solution. Mix thoroughly. Vitamin K 1 Solution: Composition per 100.0mL: Vitamin K 1 1.0g Ethanol 99.0mL Preparation of Vitamin

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