Degradation of bacteria escherichia coli by treatment with ar ion beam and neutral oxygen atoms

... DEGRADATION OF BACTERIA ESCHERICHIA COLI DEGRADATION OF BACTERIA ESCHERICHIA COLI BY TREATMENT WITH Ar ION BEAM AND NEUTRAL OXYGEN ATOMS UNI^EVANJE BAKTERIJ ESCHERICHIA COLI S CURKOM IONOV Ar ... ions and neutral oxygen atoms. The source of ions was an argon ion gun with the ion energy of 1 keV and the flux of3 ×10 18 m –2 s –1...

Ngày tải lên: 18/05/2014, 20:33

... between the total concentration of ligand in solution [L] tot and the fraction of unbound ligand [L]: ½PL¼½L tot À½Lð2Þ L. Martino et al. Structure and interactions of SlyD FEBS Journal 276 (2009) ... the detection of amide chemical shift perturbations caused by direct binding or conformational changes induced by ligand interaction, and can therefore demarcate the regions...

Ngày tải lên: 30/03/2014, 01:20

... Superposition of the E138A trimer in complex with dCTP (grey) and dTTP (yellow, cyan and magenta). (B) Stereo- view of a superposition of one of the sub- units of the E138A variant of E. coli dCTP deaminase ... Department of Molecular Biology, University of Copenhagen, Denmark Synthesis of dTMP by thymidylate synthase proceeds by the reductive methylation of dU...

Ngày tải lên: 18/02/2014, 16:20

... DYm disruption correlated, in a CAG repeat expansion- dependent manner, with an increased level of intracel- lular ROS and with increased formation of carbonyl residues in proteins. The enhanced oxidation ... with the cleavage of mutated htt and the release of an N-terminal fragment bearing the mutation that is capable of nuclear localization [6,7]. HttEx1-polyQ N-terminal...

Ngày tải lên: 19/02/2014, 06:20

... http://www. blackwellpublishing.com/products/journals/suppmat/EJB/ EJB4346/EJB4346sm.htm Details of the construction of the synthetic s-CYPA gene, purification of cyclophilin A and expression and purification of T7 RNA polymerase a re given and reference to the following supplementary ... BL21(DE3) recA/ pKO1166. Construction of pKO1166, and the production and purification o...

Ngày tải lên: 16/03/2014, 18:20

... gene expression in comparison with the action of kasugamycin. Growth of CVR40D and that of its parental strain MG1655 were compared in the presence of kasugamy- cin (Fig. 5A). Addition of kasugamycin ... Bucheli-Witschel M, Hagg P, Abdulkarim F & Isaksson LA (2004) Generation and characteriza- tion of functional mutants in the translation initiation factor IF1 of Esch...

Ngày tải lên: 29/03/2014, 09:20

... those with strong absorption bands, the evaluation of the band parameters (peak position, band width and intensity) was performed with the original spectra, if necessary after the subtraction of strong ... subtraction of baselines resulting from neighboring bands. Similar to the deconvo- lution technique, the bandshapes of the single components are superpositions of Gaussian...

Ngày tải lên: 31/03/2014, 08:20

... 930–934. 2.Erni,B.,Zanolari,B.&Kocher,H.P.(1987)Themannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA. ... curves for inactivation by 1a (circles )and iodoacetamide (stars). Fig. 4. Inhibition of sugar uptake by starved cells. EII Glc...

Ngày tải lên: 21/02/2014, 01:21

... Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL Elena S. Bochkareva, Alexander S. Girshovich and Eitan Bibi Department of Biological ... purification, and characterization of UP12 and its interaction with GroEL In order to investigate the suggestion that UP12 is a functional member of the universal st...

Ngày tải lên: 22/02/2014, 07:20

... [ 32 P]ATP[cP] (1 MBqÆlmol )1 ), with or without 2.5 mm pNPP. The phosphorylation reaction was started by addition of the enzyme, followed for 30 s at 0 °C and stopped by the addition of 0.1 mL ice-cold 5% ... FEBS Stimulation of p-nitrophenylphosphatase activity of Na + ⁄ K + -ATPase by NaCl with oligomycin or ATP Haruo Homareda 1 and Makoto Ushimaru 2 1 Department of...

Ngày tải lên: 07/03/2014, 16:20