epidemiology of verocytotoxigenic escherichia coli vtec in ruminants

... isoleucine, leucine, phenylalanine, proline, serine, threonine, tyrosine, and valine for [U-13C]glucose experiments and aspartate, isoleucine, leucine, phenylalanine, serine, threonine, tyrosine, ... OAA(1)2) Amino acid Serine U Glycine U Serine(1)3) Serine(1)3) Glycine(1)2) Glycine(1)2) Serine(2)3) Serine(2)3) Serine(1)2) Serine(1)2) Substrate Glucose U Glc,nU Glc,n1 Glc,nunlabeled not single ... similar in the three wild-type strains with small differences in the fractions of serine originating from glycine and OAA originating through the TCA cycle (Fig 5), major changes were seen in the...

Ngày tải lên: 20/02/2014, 23:20

... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this ... response of Escherichia coli Curr Opin Microbiol 2, 148–152 12 Nystrom, T & Neidhardt, F.C (1992) Cloning, mapping and ¨ nucleotide sequencing of a gene encoding a universal stress protein in Escherichia ... Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was...

Ngày tải lên: 22/02/2014, 07:20

... decarboxylated again (Fig 2) The remaining EFMs are capable of producing all of the main metabolites that we are interested in Obviously, in order to produce one of the forms of lipid A or phospholipids, ... pathway analysis of amino acid metabolism in E coli was also indicative of high redundancy [49] For analysis of robustness, rather than of redundancy, the number of EFMs remaining after knockouts ... Plotkin JB (2009) Coding-sequence determinants of gene expression in Escherichia coli Science 324, 255–258 Oefner C, Schulz H, D’Arcy A & Dale GE (2006) Mapping the active site of Escherichia coli...

Ngày tải lên: 22/03/2014, 21:20

... protein of enterohaemorrhagic Escherichia coli interacts directly with a-catenin Cell Microbiol 4, 213–222 24 Chen Y-H, Yang JT & Martinez HM (1972) Determination of the secondary structures of ... protein from enteropathogenic Escherichia coli with similarities to VirA of Shigella flexneri Infect Immun 69, 4027–4033 Kenny B & Jepson M (2000) Targeting of an enteropathogenic Escherichia coli ... secretion and activity of type III-secreted proteins of enterohaemorrhagic Escherichia coli O157 Biochem Soc Trans 31, 98–103 Kenny B, DeVinney R, Stein M, Reinscheid DJ, Frey EA & Finlay BB (1997)...

Ngày tải lên: 30/03/2014, 16:20

... médecine vétérinaire, Université de Montréal, Canada Introduction Escherichia coli is an important cause of a wide range of diseases in production animals and humans worldwide, including pigs In ... case for tetracycline, ampicillin, and sulfa drug use in both countries, and ceftiofur and apramycin use in Canada, and enrofloxacin use in Vietnam Frequent usage of agents in the past, such ... Gentamycin 14 Enrofloxacin 20 Florfenicol 12 32 Trim-Sulfa 48 82 Neomycin 23 18 Tetracycline 95 92 Ce fti of A m ur pi ci A p llin m yc G in en ta m yc Ne in om yc En in ro flo xa c in Fl or fe Sp...

Ngày tải lên: 21/06/2014, 06:20

... characterizations of Vietnamese strains of coli causing diarrhoea in pigs in Vietnam E Do NT1, Trott DJ2, Desautels C3 , Fairbrother JM3 1Department 2School 3The of Bacteriology, National Institute of Veterinary ... Woodward MJ Laboratory diagnosis of Escherichia coli infections In: Gyles CL, editor Escherichia coli in Domestic Animals and Humans Wallingford, England: CAB International; 1994 p 595-628 AIDA-I ... EFFICACY TESTING OF A VACCINE FOR THE CONTROL OF PRE-WEANING COLIBACILLOSIS IN VIETNAM Cu HP1, VA2 , Fahy Driesen SJ2, Moore K2, Vanderfeen A2, Do NT1, and Trott DJ3 1National Institute of Veterinary...

Ngày tải lên: 22/06/2014, 12:20

... galli and E coli infections including a control group (Thrusfield 1995) Ascaridia galli and Escherichia coli infections in chickens Determination of dose and infection route of E coli Fifty-two ... and Escherichia coli infections in chickens Conclusion In conclusion combined infections of A galli and E coli can have a significant impact on confined and free-range chickens keeping in mind ... that both infections are common in such production systems Further studies are needed in order to determine the underlying mechanisms of combined infections of A galli and E coli in growing and...

Ngày tải lên: 12/08/2014, 18:21

... reactivity of a second site Also pointing in this direction is the second-order or biphasic shape of the inactivation curve of EIIGlc by 1a (see residuals in Fig 3) This may indicate that binding of ... inhibition detected Half inhibitory concentrations (IC50) are given in mM Values in parentheses determined measuring inhibition of phosphorylation of [14C]a-MGlc (0.5 mM) IC50 Inhibitor Fig Inhibition ... Reversible inhibition of EIIGlc Eadie–Hofstee plots of nonvectorial phosphorylation of D-Glc (0–2 mM) by membrane fractions containing EIIGlc Phosphorylation was assayed in the presence of inhibitors...

Ngày tải lên: 21/02/2014, 01:21

... the intrinsic thermal stability of proteins [38–40] These include tighter hydrophobic packing of the protein core [41–43], increased electrostatic interactions on the surface of the protein [44,45], ... more prolines and alanines [46,47], and increased hydrogen bonding of the polypeptide chain [48–50] In addition, improved intersubunit contacts within oligomeric proteins contribute to protein stability ... to be the Tm of the protein These fibrils are amyloidogenic, as determined from their Stability and fibril formation of TTR and TRP tinctorial properties in the ThT-binding and CR-binding assays...

Ngày tải lên: 16/03/2014, 01:20

... bacterial invasion, and that the distribution of TJ proteins in TJ membrane microdomains is altered in response to EPEC infection EPEC invasion has a significant effect on localization of TJ proteins in ... penetration of EPEC through TJ membrane microdomains was related to infection We examined the role of a known lipid raft protein, flotillin-1, in EPEC invasion using a specific probe for flotillin-1 As ... flotillin-1 As shown in Fig 1, we found that flotillin-1 is intimately associated with invading bacteria at the point of attachment in EPECinfected cells Accumulation of flotillin-1 was observed at...

Ngày tải lên: 16/03/2014, 04:20

... the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution ... hydrophobic region of the c subunit, which is highly conserved but not involved in nucleotide binding or intersubunit interactions, is indicated as a potential binding site for the N-terminal disordered ... terminus of w interacts with the proposed binding region of c inside the C-terminal collar of the clamploader complex conservation on the surfaces of the d and d¢ subunits suggests that the docking...

Ngày tải lên: 16/03/2014, 16:20

... situation, the main effect of transamination is a reduction of the degree of 15N-labelling in the amino acid of interest In the case of [15N]Gly, much of the label appeared to have been trapped in an amide ... Km values of the aminoacyl-tRNA synthetases (Table 1) and the number of amino acids present in the protein The data of Fig provide a guideline for a corresponding rebalancing of amino acid concentrations ... from the amino-terminal amide protons Interestingly, ESI mass spectrometric analysis of hCypA produced in vivo in E coli also showed N-terminal heterogeneity Although  70% of the protein started...

Ngày tải lên: 16/03/2014, 18:20

... recruiting extra metalbinding capacity to the system In support of this hypothesis, whereas the PPIase domain of SlyD is required to interact with an N-terminal proline-containing stretch of HypB, ... established in this work [31] Intriguingly, our investigations reveal that this C-terminal helix, unprecedented for the FKBP family of proteins, is involved in nickel ion binding, causing conformational ... Pluckthun A (1994) An Escherichia coli protein consisting of a domain homologous to FK506-binding proteins (FKBP) and a new metal binding motif J Biol Chem 269, 28952901 Roof WD & Young R (1995)...

Ngày tải lên: 30/03/2014, 01:20

... FhuA Reduction of [55Fe3+]ferrichrome binding probably resulted from the loss of four binding sites delivered by the cork domain out of a total of 10 ferrichrome binding sites in FhuA This result ... overlaid with mL TY soft agar containing 108 cells of the strain to be tested The colicin M solution was a crude extract of a strain carrying plasmid pTO4 cma cmi [24] The microcin J25 solution was ... activities of the b-barrels [21] FhuA transport protein of E coli (Eur J Biochem 269) 4949 To gain further insights into the mode of action of FhuAD5–160, and in particular to the transport activity of...

Ngày tải lên: 31/03/2014, 08:20

... refolding of a7 extracellular domain (Eur J Biochem 269) 2805 Fig Interaction of fusion proteins containing a7 ECD with iodinated a-neurotoxins (A) Autoradiography of 125I-labelled a-cobratoxin binding ... standard proteins dissolved in the same buffers as the expressed proteins under examination Binding experiments 125 I-Labelling of a-bungarotoxin (aBgt) and a-cobratoxin (for Western blotting experiments) ... protein were obtained to perform further studies Determination of protein concentration Protein concentrations were determined using the Bradford Protein assay (Bio-Rad) with bovine serum albumin...

Ngày tải lên: 31/03/2014, 23:20

... The source of Ar ions is a commercial ion gun used for sputtering of materials during depth profiling Ar ion beam with the energy of keV at an incidence angle of 45° and a raster of mm × mm was ... DEGRADATION OF BACTERIA ESCHERICHIA COLI EXPERIMENTAL 2.1 Sample preparation Bacteria Escherichia coli (E coli) were cultivated according to the standard procedure In experiment we used bacteria E coli ... DEGRADATION OF BACTERIA ESCHERICHIA COLI from radiation damage, the argon ions caused sputtering of the bacterial material The sputtering was extremely inhomogeneous what was explained by local charging...

Ngày tải lên: 18/05/2014, 20:33

... already individually performed vaccinations of single animals, as the latter usually targets only newly introduced animals This suggested combination of mass vaccination of all large ruminants ... appropriate vaccination strategy for Pakistan's largest dairy colony, including the choice of the best matching vaccines, as well as helping to improve our understanding of the epidemiology of FMD http://www.virologyj.com/content/5/1/53 ... newly introduced animals This suggested combination of mass vaccination of all large ruminants with the already performed individually vaccination should provide a continuous high level of herd...

Ngày tải lên: 20/06/2014, 01:20

... EU034714] obtained from a sample of swine serum in eastern China during the same period, suggesting that the isolates had a common origin [13] To date, a total of nine subtype 4i HEV strains identified ... and its neighboring provinces, the epidemiology of HEV infections within the municipality, a major industrial and commercial center, deserves closer attention Findings A total of 65 sequences, ... prevalent subtypes Of the 29 sequences classified as subtype 4i, four were of human origin and 25 were from swine HEV strain SH-SW-zs1 [GenBank: EF570133], originating from swine and regarded as...

Ngày tải lên: 11/08/2014, 21:22

... was introduced into Korea around 1991 (K91P55 strain) or 1994 (K94P05 strain) [14,15,27] Interestingly, this genotype change was also reported in Japan in 1991 [12,23], in China in 1979 [13], in ... isolated in China, Japan, India, Philippines, Sri Lanka, and Vietnam between the 1930 s and the early 1990 s Another 10 Korean strains clustered in genotype 1, together with strains isolated in China, ... Moreover, in an analysis of Flavivirus recombination, K82P01 and K91P55 appeared to be putative recombinant strains derived from genotype and strains [24] When we analyzed the two strains using the...

Ngày tải lên: 12/08/2014, 04:20

... first finding is incidence of HCV genotypes that confirms the 36 P value NS NS findings of another study from this country [23] The second important finding of the study was the isolation of 27% ... non-disposable syringes for injections attended a number of patients in the past Mass vaccination in the recent past in which un-sterilized syringes were used might have enhanced the infection rate in this ... Kingdom with the widespread use of heroin in the 1960s [29] For more than 58% of our patients the probable modes of transmission observed were multiple uses and re-uses of needles/syringes In...

Ngày tải lên: 12/08/2014, 04:20