proteolytic enzymes, serine and cysteine peptidases

... Group of Peptidases EC sub-subclass Carboxypeptidases Serine- type carboxypeptidases Metallocarboxypeptidase s Cysteine- type carboxypeptidases Endopeptidases Serine endopeptidases Cysteine ... recognition of a serine peptidase is 3,4-DCI. This is safe and convenient to use, and reacts rapidly and irreversibly with a wide range of serine peptidases (see [31] in thi...

Ngày tải lên: 11/04/2014, 10:13

... and Molecular Biology. In Volume 244 on peptidases of serine and cysteine type and in this volume on aspartic, metallo, and other peptidases, the chapters on spe- cific methods, enzymes, and ... DMSO, and at 25 ° for factor D, C2a~ and Bb, and 0.1 M HEPES, 10 mM CaC12, buffer, pH 7.5, 9% DMSO, and 30 ° for Clr and Cls. The units for kcat, Kin, and kcat/Km...

Ngày tải lên: 11/04/2014, 10:13

... The amino acid sequence enclosed in dark boxes and in open boxes are common sequences among ALP I and the neutral subtilisins, and among ALP I and the alkaline subtilisins, respectively. s, Unique amino ... Two mutant enzymes, D266N/Y269A- and D266N/Y269A/A271T/ Q272R-ALP I, were most stable retaining 60% of the original activity after 1 h, and 30% after 6 h. 2 The D266N- ALP I...

Ngày tải lên: 21/02/2014, 03:20

... atherosclerosis and acute lung injury. Both ACE and ACE2 have catalytic activity that is chloride sensitive and is caused by the presence of the CL1 and CL2 chloride-binding sites in ACE and the CL1 site ... the CL1 and CL2 site residues are responsible for chloride sensitivity. The CL1 site resi- dues Arg186, Trp279 and Arg489 of testicular ACE and the equivalent ACE2 residue...

Ngày tải lên: 16/03/2014, 04:20

... December 2006) doi:10.1111/j.1742-4658.2007.05666.x Papain-like cysteine proteases of pathogenic protozoa play important roles in parasite growth, differentiation and host cell invasion. The main cysteine proteases of Trypanosoma cruzi (cruzipain) and ... of papain-like enzymes, the propeptide was incubated for 30 min with papain or human cathepsins B, L, S, K, V and F (in the sub...

Ngày tải lên: 16/03/2014, 11:20

... incidents involving anesthesia. To under- stand the role of citrulline-NO cycle enzymes, GS and the oxidative status in anoxia and reperfusion, NOS, AS, AL, GS and arginase activities along with the ... acid transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and...

Ngày tải lên: 26/10/2012, 09:07

... 13% and 21% increase in the xylanase and cellulase production, respectively. The optimum temperatures of the crude cellulase and xylanase were 60 0 C and 70 0 C with half-lives of 15 h and ... degradation and conversion for sustainable production of bioenergy and high- value products; enzyme production and catalysis, bioremediation and biorefineries. Lew is the author of 7...

Ngày tải lên: 05/09/2013, 16:11

... overweight and obese _______ ∗ Corresponding author. Tel.: 84-4-38582179. E-mail: liendn@vnu.edu.vn persons and over 200 million persons suffered from diabetes mellitus (DM), and this number ... hypoglycemic and hypolipidemic activity have been described in D.N. Lien et al. / VNU Journal of Science, Natural Sciences and Technology 27 (2011) 118-124 119 the traditional re...

Ngày tải lên: 12/02/2014, 17:20

... stability Temperatures of maximum proteolytic activity (T max ) for rproErv-C +CT and rproErv-C DCT are 50 and 45 °C (Table 2 and Fig. 4) and they retain > 90% activity up to 65 and 60 °C, respectively. ... experiment using three latex enzymes Erv-A, -B and -C from the same plant, and papain from papaya latex and two other serine (tryp- sin and chymotrypsin) and one...

Ngày tải lên: 14/02/2014, 14:20

... Kunitz-type serine protease inhibitor. J Biol Chem 272, 6370–6376. 5 Lin C-Y, Anders J, Johnson M & Dickson RB (1999) Purification and characterization of a complex containing matriptase and a Kunitz-type ... implicated in prostate and ovarian cancers. Biochem J 390, 125–136. 11 Kim DR, Sharmin S, Inoue M & Kido H (2001) Cloning and expression of novel mosaic serine proteas...

Ngày tải lên: 15/02/2014, 01:20