glycine serine and threonine

... PAGE and eluted in H2O An aliquot was then evaporated to dryness and 5.5 M HCl was added for h at 110 °C The hydrolysate was evaporated, mixed with unlabeled pSer, pThr and pTyr standards and ... p53S15A/S37A was eluted from a 40% alkaline PAGE gel and hydrolyzed in HCl Phosphoamino acids were resolved by TLC in the presence of phosphoserine and phosphothreonine markers Assignment of phosphorylation ... phosphorylation was made by superimposition of autoradiographs and ninhydrin staining, with the position of phosphoserine (pSer) and phosphothreonine (pThr) migration indicated to the left of the phosphorimage...

Ngày tải lên: 30/03/2014, 15:20

... concentrations of ATP and homoserine Two different coupling systems were used in order to measure threonine and cystathionine ux Threonine ux was measured using puried threonine deaminase and commercial ... between the methionine and threonine biosynthesis pathways occurs at the level of homoserine and involves dierent enzymes and dierent allosteric patterns [8] of methionine (Fig 1) and can be considered ... the activity of both CGS and TS [12,17]; (f) the enzymatic products cystathionine and threonine not inhibit the activities of CGS [9,12] and TS [13,16] and (g) nally and importantly, Phser is...

Ngày tải lên: 20/02/2014, 02:21

... aspartates, and cysteines in the rat Ntcp are conserved in all of the nine family members: Cys44, Cys98, Cys125, and Cys266 as well as Glu47, Asp115, Asp147, Glu257, and Glu277 The threonines Thr317 and ... basolateral (Ntcp rat [1], Ntcp mouse1 and [9], Ntcp rabbit [24], and NTCP human [2]) and four apical (Isbt rat [6], Isbt mouse [25], Isbt rabbit [26] and ISBT human [27]) was performed, using ... reactions as it was shown that the Ntcp is a serine/ threonine phosphorylated phosphoprotein which is dephosphorylated by cAMP [22] Upon phosphorylation of serine/ threonine by a protein kinase A, taurocholate...

Ngày tải lên: 17/03/2014, 09:20

... recognition of a serine peptidase is 3,4-DCI This is safe and convenient to use, and reacts rapidly and irreversibly with a wide range of serine peptidases (see [31] in this volume) Serine peptidases ... alignment with the mean and standard deviation of scores for random shuffles of the sequences A score of at least six standard deviation units above the mean for the random shuffles is required ... activity are widespread and very numerous Serine peptidases are found in viruses (Table I), bacteria, and eukaryotes, and they include exopeptidases, endopeptidases, oligopeptidases, and omega peptidases...

Ngày tải lên: 11/04/2014, 10:13

... characters and six loci SSR in the early segregating generation from the cross between wild soybean and cultivated soybean Materials and methods 2.1 Materials + Xanh Ha Bac cultivar (Glycine max, landrace) ... species of soybean (Xanh Ha Bac and Soja 182) on 3% agarose gel Almost markers could successfully amplify DNA and generated clear bands, except Satt173 and Satt243 Bands sizes had slight difference ... (Glycine soja, Korea origin) + F2 population of the cross between Xanh Ha Bac (as female parent) and Soja 182 (as male parent) + Chemicals and equipments for planting, DNA extraction, PCR, and...

Ngày tải lên: 06/10/2015, 13:05

... overexpression, and characterization of a serine ⁄ threonine protein kinase pknI from Mycobacterium tuberculosis H37Rv Protein Expr Purif 36, 82–89 13 Villarino A, Duran R, Wehenkel A, Fernandez P, England ... Mycobacterium tuberculosis serine ⁄ threonine kinases PknA and PknB: substrate identification and regulation of cell shape Genes Dev 19, 1692–1704 16 Deol P, Vohra R, Saini AK, Singh A, Chandra H, Chopra ... Tyagi AK, Drlica K, Singh Y & Ullrich A (2001) Serine ⁄ threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization and localization Microbiology 147, 2307–2314 12...

Ngày tải lên: 16/03/2014, 14:20

... extract (lanes and 5), cytosolic fraction (lanes and 6) and membrane fraction (3 and 7) of S pneumoniae strains Purified recombinant StkP was used as a control (lane 4) Arrows indicate bands corresponding ... PAGE, and radioactive bands revealed by autoradiography Positions and molecular mass (kDa) of protein standards are indicated on the left The arrows indicate the position of phosphorylated rStkP and ... mutation and a silent NaeI site) and PMUT2 (which introduced the D231A mutation and a silent StuI site) and reverse primer PHPP-R in the first round of PCR The generated fragments (190 and 75 bp,...

Ngày tải lên: 16/03/2014, 18:20

... disrupted promoter structure and exons I and II, exhibit impaired follicular development and ovulation [25] and impaired spermiogenesis in males [26] On the other hand, mice defective in Camk4 ... hemisphere and between cerebellum primordium and medulla oblongata (Fig 6, A1 and A2) Also border regions between metencephalon and pons with the third ventricle (arrows in Fig 6, A1 and A2) showed ... fetal heart, pituitary gland and kidney Weak but still above background signals were found in interventricular septum of the heart, pancreas, trachea, and thyroid gland (Fig 1B,C) Hybridization...

Ngày tải lên: 16/03/2014, 23:20

... rapid and strong activation of PKB (also known as c-Akt) [28] and this is mediated by phosphatidylinositol kinase (PtdIns3K) and PDK1 Thus A14 cells were transfected with HA-tagged TSSK3 and treated ... with substitution of Ser5 and Ser6 to Ala and peptide 4(S4,6A), KRRASAYHV with substitution of Ser4 and Ser6 to Cys Oligonucleotides contained EcoRI overhang on 5¢ site and NotI overhang on 3¢ ... (A14) and HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) (Sigma) and 1% antibiotic suspension (penicillin and streptomycin; Sigma) and...

Ngày tải lên: 23/03/2014, 11:20

Ngày tải lên: 07/08/2014, 15:20

... glycation and oxidation of proteins that form with aging, diabetes and renal failure, and at sites of inflammation (by oxidation) [16] Levels of S100 proteins and HMGB1 are increased in both adult and ... LC, IF, MT, TM and RT were involved in conception, design, acquisition, analysis and interpretation of data LC, IF, TM and RT drafted and revised the manuscript All authors read and approved the ... disease duration of 15 years and mean age of 61.8 years (range, 17 to 87 years) for CV events, duration and features of RA, CV disease risk factors, and HLA–DRB1 and RAGE Gly82→Ser genotypes...

Ngày tải lên: 09/08/2014, 10:20

... and different MAPK pathways and Akt, as well as the role of different MAPK pathways, extracellular signal-regulated kinase (ERK) 1/2 and p38, and Akt, we employed pharmacological inhibition and ... Akt and MAPK pathways Materials and methods Experimental animals Male C57BL/6 mice, either wild-type Akt+/+ or Akt+/- on a C57BL/6 background, aged between and weeks, weighing between 20 and ... damage to the lung structure and formation of fibrous bands or small fibrous masses; grade = severe distortion of the structure and large fibrous areas (honeycomb lung); and grade = total fibrous...

Ngày tải lên: 13/08/2014, 11:22

... commercial germplasm and selection was focused on high yielding genotypes and high seed protein and oil In effect, this leads to narrow genetic base of soybean cultivars (Singh and Hymowitz, 1999) ... ĐT12 and ĐT20 for selection towards improving yield MATERIALS AND METHODS Seeds of three soybean cultivars ĐN6, ĐT12 and ĐT20 were obtained from Center for National Testing of Plant Products and ... agronomical and yield contributing traits in soybean (Glycine max (L.) Merrill) with gamma irradiation season Based on the effect and effectiveness of mutagen in terms of survival and growth reduction,...

Ngày tải lên: 29/08/2013, 08:14

... Kunitz-type serine protease inhibitor J Biol Chem 272, 6370–6376 Lin C-Y, Anders J, Johnson M & Dickson RB (1999) Purification and characterization of a complex containing matriptase and a Kunitz-type serine ... membrane-anchored serine protease implicated in prostate and ovarian cancers Biochem J 390, 125–136 Kim DR, Sharmin S, Inoue M & Kido H (2001) Cloning and expression of novel mosaic serine proteases with and ... TMPRSS13 mRNA was detected in MKN45 and MKN74 cells, but not in A549, LoVo and HepG2 cells On the other hand, HAI-1 mRNA was detected in LoVo, MKN45, MKN74 and HepG2 cells (Fig 6) These results...

Ngày tải lên: 15/02/2014, 01:20

... incubated with 10 mM glycine and 500 lM FTHF for and then dialyzed against buffer D not containing glycine and FTHF Such an enzyme was once again incubated with glycine (10 mM) and different concentrations ... E53QbsSHMT-Gly(FTHF) against the buffer not containing glycine and FTHF and determined the slope for the formation of quinonoid intermediate by the addition of 10 mm glycine and varying FTHF concentrations As shown ... group and contained one monomer in the asymmetric unit Cell dimensions and details of data collection are shown in Table Expression and purification of bsSHMT and E53QbsSHMT The expression and purification...

Ngày tải lên: 18/02/2014, 16:20

... the Soret band maximum of heme–GmHO-1 immediately shifted from 405 to 415 nm, and at the same time, distinct absorption bands of oxyheme appeared at 540 and 579 nm Then, a broad band appeared ... identity, and heavily shaded histidine residues are proximal heme ligands Bars below the alignments show a-helical parts (AH) in the crystal structures of heme–SynHO-1 and heme–rHO-1 and those ... frequencies, 100 kHz; field modulation amplitude, 10 G for (A) and (B) and G for (C)–(E); signal acquisition temperature, K for (A) and (B) and 25 K for (C)–(E) (A) The ferric heme complex of GmHO-1...

Ngày tải lên: 19/02/2014, 05:20

... enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln [49] Serine metabolic pathways are often absent in parasitic ... active site cleft and is involved in substrate binding and serine inhibition as shown previously with the Gly294Ala or Val mutation, which affected Km and cooperitivity of serine inhibition [42] ... shown to be implicated for cooperativity in serine binding and serine inhibition, and an adjacent Lys141/Arg141, both of which are conserved among Type I and Type II sequences All the other important...

Ngày tải lên: 19/02/2014, 13:20

... of serine proteinase inhibitors against proteolysis of the 55-kDa Tm-mas and PO activity by using two kinds of serine proteinase inhibitors, such as p-NPGB and p-APMSF As shown in Fig 7C and ... TCA and subjected to SDS/PAGE and then immunoblotting with the affinity-purified antibody raised against the 45-kDa Tm-mas solution was preincubated with p-NPGB and p-APMSF, and then calcium and ... Masquerade-like serine proteinase homologue (Eur J Biochem 269) 4381 Fig Western blotting of (A) Tenebrio 79-kDa pro-PO, and (B) 55-kDa Tm-mas in HW-55S solution by incubation with Ca2+ and b-1,3-glucan and...

Ngày tải lên: 21/02/2014, 03:20

... antibodies against phosphoserine and phosphothreonine Both antibodies recognized PknA, suggesting that the phosphorylated residues are serine and threonine (Fig 1E, lanes and 4) However, both antisera ... (lanes and 4) after Western-blot analysis with antibodies to phosphothreonine (left panel) and phosphoserine (right panel) Numbers denote size of the molecular mass standards compare lanes and 3) ... (anti-MBP, anti-phosphoserine, antiphosphothreonine and anti-phosphotyrosine) used for different immunoblots were commercially available (New England Biolabs, Santa Cruz Biotech and Sigma) Horseradish...

Ngày tải lên: 22/02/2014, 04:20

... sites in both Ggly and Gamide, with a Kd of 0.6 lm in aqueous solution [11] Glu7 serves as a ligand for one ferric ion, and Glu8 and Glu9 bind a second ferric ion, in both Ggly [12] and Gamide [13] ... that Glu7 acts as a ligand for the first ferric ion, and that Glu8 and Glu9 act as ligands for the second ferric ion, in the gastrin– ferric ion complex for both Ggly [12] and Gamide [13] To characterize ... constants for association (ka) and dissociation (kd) were as follows: for Gamide, ka = 5.94 · 105 m)1Æs)1, and kd = 8.06 · 10)4 s)1, and for Ggly, ka = 5.20 · 105 m)1Æs)1, and kd = 1.06 · 10)3 s)1...

Ngày tải lên: 07/03/2014, 02:20