protein - dna interactions

... widely used in the study of DNA binding proteins. These include purifica- tion and protein characterization, assays of protein- DNA binding and protein- induced DNA bending which can be used ... cellular protein. Fortunately, it is now possible to purify sequence-specific DNA- binding proteins by DNA affinity chromatography. DNA affinity chromatography was originally devel...

Ngày tải lên: 11/04/2014, 10:11

... ATP-induced aggrega- tion even in the absence of DNA might form the basis of such massive MutS–MutL-heteroduplex DNA com- plexes. The hydrodynamic size of such particles con- taining protein DNA complexes ... MutS–MutL -DNA com- plexes are formed by adding duplex DNA to a mixture containing MutS and MutL. In experiments involving nucleotide cofactors such as ATP or ATPcS, MutS -DNA...

Ngày tải lên: 07/03/2014, 12:20

... promoter DNA. WT DevR protein bound to fdxA promoter DNA over a narrow range (< 10-fold) of protein concentration; at 500 nm concentration > 90% saturation of DNA was observed with WT DevR protein ... promoter (up to 6.0 lm protein concentration) in a DNase I footprint- ing assay. This property established that the K182G mutant protein was defective in sequence-specifc in...

Ngày tải lên: 22/03/2014, 16:20

... PCR using error-checking DNA poly- merase (TakaraEx, Otsu, Japan). pF10.His (full-length Cph1 with a C-terminal His-tag) has been described else- J. Hahn et al. Protein chromophore interactions ... inset: UV-Vis-spec- trum of chromopeptide peak. (B) MALDI-TOF spectrum of chromo- peptide fraction; inset: enlarged. (C) MALDI-TOF ⁄ TOF spectrum; inset: enlarged. Protein chromophore intera...

Ngày tải lên: 30/03/2014, 11:20

... flexible in the com- plex with the low-affinity DNA, and structured into an a-helix in the complex with the specific DNA. There- fore, the large local conformational landscape that the protein populates ... no structure for the DNA- binding domain, the superhelical assembly suggests the forma- tion of a complex with plectonemic DNA. Engineering and design of protein- nucleic acid intera...

Ngày tải lên: 14/02/2014, 19:20

... handling. 8. 3-mm gel comb with 10-mm-wide teeth. 9. 10-mL syringe and 18-gage needle. 10. 10 0- to 20 0- L Hamilton syringe. 11. Additional reagents and equipment: Powdered acrylamide and N,N'-methylene– bis-acrylamide ... Assays for the Analysis of DNA -Protein Interactions Marc-André Laniel, Alain Béliveau, and Sylvain L. Guérin 1. Introduction Several nuclear mechanisms inv...

Ngày tải lên: 08/04/2014, 12:51

... DNA- fragment. Lane 3: Labeled DNA- fragment, after Exo III treatment. Lane 4. Pattern of DNA -protein complex, obtained after Exo III treatment with both S- ends labeled. Lane 5: Pattern of DNA -protein ... the kind of protein- DNA complexes you are inves- tigating, several modifications of the procedure described in the pre- vious section may be useful or necessary. If the p...

Ngày tải lên: 11/04/2014, 09:40

... inhibition of the key proteins ataxia telan- giectasia related (ATR) and ataxia telangiecta- sia-mutated (ATM). The DNA- nicking activity that NS1 uses to separate viral genomes would be ex- pected to ... sufficient DNA nicks to ac- tivate this repair pathway. To study the importance of the PARP-initiated DNA repair pathway in NS1-induced apoptosis, the cell-permeable PARP inhibitor...

Ngày tải lên: 25/10/2012, 11:18

... 14 6-1 51 146 International Journal of Medical Sciences ISSN 144 9-1 907 www.medsci.org 2004 1(3): 14 6-1 51 ©2004 Ivyspring International Publisher. All rights reserved Expression of hMSH2 protein ... misparing and slippage errors in DNA synthesis. The mismatch is detected by two complexes, the hMSH2-GTBP/hMSH6 and the hMSH2-hMSH3 heterodimers after interaction with hMLH1-h...

Ngày tải lên: 03/11/2012, 09:54

... mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding protein RBP16 [62], and the Escherichia coli protein StpA [63], an inverse or miss- ing ... way, and to acceler- ate structural transitions in RNAs and RNP complexes [37]. DEAD-box proteins also disrupt RNA protein interactions [38,39], and some have been shown to pro- mote...

Ngày tải lên: 14/02/2014, 19:20