protein surface interactions in the presence of poly ethylene glycol

... undetectable in the presence of Mn2+ The solvent-exposed C-terminus of VpUcyt probably pointed away from the surface of the micelle The three amide cross-peaks originating from the hydrophobic ... K, Hoffmann S, Bauer F, Friedrich U, Kardinal C, Feller SM, Biesinger B & Sticht H (2002) Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase ... 10 The orientation of the presented molecule was manually adjusted to reflect the PRE data in the following way: green-coloured regions of the protein that appear to be close to the core of the...

Ngày tải lên: 18/02/2014, 06:20

... activity of the reaction intermediate, FVaint This facilitated the determination of loss of FVa cofactor activity during the initial stage of inactivation and minimized the in uence of the k306 ... part of the heparin-binding site in these three crystallized protein heparin complexes were given as starting point for the docking search [30] At least one conformation of the simulated protein peptide ... time the loss of FVa activity in the absence of heparin (open circles) did not differ from that obtained in the presence of heparin (closed circles) This indicates that the presence of heparin...

Ngày tải lên: 19/02/2014, 13:20

... hydration of the protein domain is the driving force of protein stabilization or folding [3,4] Furthermore, the effect of osmolytes on the functional activity of an enzyme has also been investigated ... and DGD° values in the presence of these osmolytes are given in Table However, DGD° values of RNase-A in the presence of alanine, serine, lysine, b-alanine, taurine and dimethylglycine have not ... efficiency in the presence of this group of osmolytes Interestingly, there is a linear relationship between DDGD° and Dlog(kcat ⁄ Km) in the presence of methylamines and sugar However, the slopes of the...

Ngày tải lên: 07/03/2014, 00:20

... softening depends on the melting of these crystallites, but can be changed by a chemical crosslinked network [5] The crosslinking influences on the softening point (Ts) of the PVC samples in comparing ... in the presence of phr of TMPTMA of the PVC crosslinked samples decreases with increasing concentration of TMPTMA up to 15 phr Therefore, in order to obtain PVC having the highest , 0.4 phr of ... improves the molecular mobility, melting and softening of PVC and a result, Ts decreases with increasing Table 1: Softening point of the PVC samples containing different concentrations of DAPC...

Ngày tải lên: 03/04/2014, 15:20

... alignment in linear space, using a sliding window, while also identifying the intersections of the corresponding path with the established cut-points; in the second step, we recompute separately the ... reverse translation of a protein usually refers to obtaining one of the DNA sequences that encodes the given protein Several methods for achieving this exist [10,11], aiming at finding the DNA sequence ... (w) are the probabilities of the word w in the reading frame imposed by the position of the first and second codon, respectively This is computed as the products of the probabilities of the codons...

Ngày tải lên: 12/08/2014, 17:20

... (C), in the presence of 100 lM MPP+ (D), in the presence of 200 lM MPP+ (E), in the presence of 100 lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the presence ... solid line), in the presence of 100 lM neurotoxin ( , dotted line), in the presence of 200 lM neurotoxin ( , dotted line) and in the presence of neurotoxin and 50 lM dopamine (h, dashed line) • 1692 ... shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 lm dopamine on the aggregation process Dopamine delayed the lag phase of aggregation marginally...

Ngày tải lên: 14/02/2014, 19:20

... self-financing portfolio if and only if certain integral equations of the first kind (the “hedging equations”) have solutions Furthermore, the integral operators appearing in these equations (the “hedging ... apply part of the present theorem We now turn to the issue of so called “martingale modelling”, and remark that one of the main morals of the martingale approach to arbitragefree pricing of derivative ... turn out to be adjoint of the martingale operators • We show that uniqueness of the martingale measure is equivalent to the denseness of the image space of the hedging operators In particular, it...

Ngày tải lên: 16/02/2014, 02:20

... identify the site of action of the fatty acids in the plasmin molecule, the amidolytic activity of two truncated plasmin variants was examined (Fig 8) Miniplasmin (des-kringle 1-4 plasmin) contains the ... resulted in a decrease, of up to two-fold, in the kp of plasmin Considering the recently described reversible nature of the plasmin inhibition by oleic acid [3] and the changes of the kinetic parameters ... catalytic action of plasmin in the presence of stearate The p-nitroaniline (P) released in the course of hydrolysis of 40 lM Spectrozyme-PL by 20 nM plasmin in the presence of 115 lM stearate is...

Ngày tải lên: 18/02/2014, 17:20

... consistent with titration of the catalytic histidine, H438(440) The persistence of activity in the presence of the protonated form of the catalytic histidine is inconsistent with the generally accepted ... form of BuChE, as indicated In each panel, the solid circles indicate the measured kcat values, the solid squares indicate the measured bkcat values, and the lines are the result of fitting the ... excess-substrate binding appears to involve the intricate interplay of a variety of residues in the active site pH dependence of A328W Fig pH dependence for the turnover number (kcat) in the absence of excess...

Ngày tải lên: 20/02/2014, 23:20

... the complexity of these drug interactions, the key role of the rifamycins in the success of tuberculosis treatment mandates that the drug-drug interactions between the rifamycins and antiretroviral ... least the first months of treatment among patients with advanced HIV disease 8, Predicting Drug Interactions Involving Rifamycins Knowledge of the mechanisms of drug interactions can help predict the ... isozymes The rifamycins vary in their potential as CYP450 inducers, with rifampin being most potent, rifapentine intermediate, and rifabutin being much less active Rifampin also upregulates the synthesis...

Ngày tải lên: 06/03/2014, 04:20

... growth of the WO3 nanowires With the addition of different inorganic salts, the compositions and the corresponding properties of the solution are different in the hydrothermal conditions The changes ... need the anisotropy during the growth process for the nanoparticles In our experiments, the presence of the salts is also an important factor influencing the crystallization process and the growth ... morphologies of the final products were demonstrated in Fig 1a–c On the basis of the SEM check, the proportion of the nanowire morphology was estimated to be about 100% (Fig 1a) As shown in the SEM...

Ngày tải lên: 20/03/2014, 13:04

... These results are largely robust to including the proportion of Group pupils in the surrounding area as the explanatory variable, rather than the total number of Group pupils in the surrounding ... treatment in 1998 This was partly because of confusion in the field about pupil age, and partly because in the early stages of the program several of the Kenya Ministry of Health nurses administered ... correlated among these individuals at the margin of receiving treatment 34 We have also calculated Manski bounds on within-school externalities in the presence of selection into treatment, but these are...

Ngày tải lên: 22/03/2014, 15:20

... of p-amindinobenzamidine Figure 2B shows time courses for trypsinogen autoactivation in the presence of 100 lM p-amindinobenzamidine As seen in this figure, the presence of p-amindinobenzamidine ... analyze the effects of acetamidine, p-amidinobenzamidine and benzamidine on the autoactivation of trypsinogen by trypsin Figure shows time courses of trypsinogen autoactivation in the presence of ... Effect of p-amindinobenzamidine concentration on Km for autoactivation of trypsinogen by trypsin gives a straight line, indicating that p-amindinobenzamidine is a competitive inhibitor for the trypsinogen...

Ngày tải lên: 23/03/2014, 13:20

... above, we examined the performance of the models using hand-processed evaluations of the PUZZLE CORPUS data The following presents the results of the three different models on 10 trials of the PUZZLE ... balance of linguistic and visual salience is needed in order to account for patterns of referring behavior In the remainder of this paper, we begin by presenting a brief discussion of the motivation ... human-agent interactions in a wide variety of contexts that include visual objects of interest Such systems may benefit from a datadriven model of how collaborative pairs adapt their language in the presence...

Ngày tải lên: 24/03/2014, 03:20

... are involved in the transmission of mechanical signals to signaling pathways In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... expression [18] The MARP family is composed of three proteins, ankyrin repeat domain-containing protein (Ankrd2), cardiac ankyrin repeat protein (CARP) and diabetes-related ankyrin repeat protein (DARP), ... Considering the location of the N-terminal extremity of DNter2 on the structure of CARP and the presence of the potential cleavage sites, we identified the localization of the cleavage site within...

Ngày tải lên: 29/03/2014, 21:20

... the parameter estimates obtained in the absence of the inhibitors (see above) In the case of gallamine investigated in the presence of MeCN, the calculation of the kinetic parameters was based ... for the inhibition of AChE by gallamine in the absence of MeCN (B) Plot of int0 (int0)1) and int1 (int1)1) versus the reciprocal concentrations of gallamine Inhibition kinetics of acetylcholinesterase ... AChEgallamine to hydrolyse ATCh is 1.7 25% of that of free AChE Inuence of MeCN on the inhibition of AChE The inhibition of AChE by tacrine, gallamine and compound was investigated in the presence of...

Ngày tải lên: 29/03/2014, 23:20

... (broken lines), either in the form of aggregates formed in the presence of Cu2+ [(i) and (iii)] or Zn2+ [(ii) and (iv)], or as fibrils formed in the presence of EDTA [(v) and (vi)] The amount of amyloid ... Error bars indicate the experimental uncertainty of the measurements observed between the Cu2+-induced and Zn2+-induced aggregates, reflecting the differences in metal-binding properties of Ab [26,30,33–35] ... formed in the presence of a molar excess of divalent metal ions failed to attain the classic morphology of amyloid fibrils [37,46] The aggregates of Ab(1–40) and Ab(1–42) formed in the presence of a...

Ngày tải lên: 30/03/2014, 01:20

... variant and that the lower fitness of the recombinant virus can not be increased by pre-passaging in cell culture The observed survival time of the recTULV in the presence of the Page of (page number ... conserved inverted repeat in the 3'-noncoding region of the S segment seems to be involved in recombi- nation events, leading, however, to the deletion of the hairpin-forming sequences (A Plyusnin, ... Figure recombinant S RNA segments Checking of specificity of RT-PCRs for the wt and the Checking of specificity of RT-PCRs for the wt and the recombinant S RNA segments Lines 1–3: products of RT-PCR...

Ngày tải lên: 18/06/2014, 22:20

... variant and that the lower fitness of the recombinant virus can not be increased by pre-passaging in cell culture The observed survival time of the recTULV in the presence of the Page of (page number ... conserved inverted repeat in the 3'-noncoding region of the S segment seems to be involved in recombi- nation events, leading, however, to the deletion of the hairpin-forming sequences (A Plyusnin, ... Figure recombinant S RNA segments Checking of specificity of RT-PCRs for the wt and the Checking of specificity of RT-PCRs for the wt and the recombinant S RNA segments Lines 1–3: products of RT-PCR...

Ngày tải lên: 20/06/2014, 04:20

... less intensive than their fully coordinated counterpart since they only require the local processing of the beamforming weights Besides the difficult exchange of the data and CSI between the transmit ... impacts of CFO in coordinated schemes are hence worse than for point-to-point communications Because the frequency offset translates into the possibly destructive combination of the incoming streams, ... the energy toward the receiver P1 obtained by averaging the instantaneous SNR over the channel realizations and indicates the SNR gain over the single-user (SU) single-input-single-output (SISO)...

Ngày tải lên: 20/06/2014, 22:20