plant gene transfer and expression protocols
plant gene transfer and expression protocols
... marker genes
for the selection of transformants, and plant promoters to achieve more
specific patterns of gene expression.
2. Expression Cassettes
Efficient expression of foreign genes in ... D R , and Howell,
S H (1986) Transient and stable expression of the firefly luctferase gene m plant
cells and transgemc plants Science 234, 856-859
18 Mullmeaux, P. M., Guer...
gene transfer and expression protocols
... Methods in Molecular Biology, Vol. 7: Gene Transfer and Expression Protocols
Edited by: E J Murray 0 1991 The Humana Press Inc., Clifton, NJ
15
16 Okayama and Chen
The calcium phosphate method ... chloramphenicol acetyl transferase (CAT) gene.
This type of gene transfer is often referred to as a transient expression system.
Production of the CAT enzyme peaks at around...
gene isolation and mapping protocols
... subsequent genettc linkage analysis,
and the calculation of gene frequencies. The ordering of several markers, if
unknown, into a genetic map, and the calculation of recombination fractions
and genetic ... Soares, M B , and Sikela, J M (1995) Gene- based
sequence-tagged-sites (STSs) as the basis for a human gene map. Nat Genet 10,
415-423.
Linkage Analysis of Genetic Disorder...
plant cell electroporation and electrofusion protocols
...
direct gene transfer in plant protoplasts. Plant Mel B~ol 8,363-373.
13. Installe, P., Negrutiu, I., and Jacobs, M. (1985) Protoplasts-derived plants in N.
plumbaginifolia: improvmg the regeneration ... wheat and barley m order to improve quality. Transgenrc
Res. 3, 3-12.
15. Gallois, P., Lindsey, K , Malone, R., Kreis, M., and Jones, M. G. K. (1992) Gene
rescue m plants...
vascular disease, molecular biology and gene transfer protocols
... Biology and Gene Therapy Protocols
Edited by: A. H. Baker © Humana Press Inc., Totowa, NJ
2
Analysis of Genetic Variants in Cardiovascular Risk
Genes by Heteroduplex Analysis
Ana Cenarro, Fernando ... methodologies and limitations, in Genetics of Common Dis-
eases (Day, I. N. M. and Humphries, S. E., eds.), BIOS Scientific Publishers,
Oxford, pp. 33–74.
2. Cooper, D. N. and Krawcz...
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... plasmid DNA containing TI gene fragment
Expression of recombinant MCoTI-II (ReMCoTI-II)
The expression conditions such as ampicillin and IPTG concentrations, temperature
and time of induction ... and Richard A. Houghten (Editors), American Peptide
Society, 2001, pp. 387-388.
2. Bozenna Rempola, T-Wilusz, W. Markiewicz and M.Fkus, Synthesis, cloning and
expression in E.col...
Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
... prostaglandin E2,
IL-10 and IL-11 generation in antigen presenting cells.
Clin Exp Immunol 129, 438–445.
7 Kernacki KA, Goebel DJ, Poosch MS & Hazlett LD
(1998) Early cytokine and chemokine gene ... decidua
and fetoplacental development in mice. Genes Dev 12,
2234–2243.
22 Wang T & Secombes CJ (2003) Complete sequencing
and expression of three complement components, C1r,
C...
Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf
... receptor gene expression cassettes (DBD:EcR and AD:RXR), whereas the
monopartite gene switch requires one receptor gene expression cassette (AD:DBD:EcR), to transactivate the reporter gene expression ... VP16:CH9 (VCH9) and luciferase expression
cassettes to generate a two-hybrid gene switch
(p2300GCfE
VY
:VCH9:Luc) and VGCfE
VY
and lucif-
erase expression cassette...
Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer
technique
Nandakumar Sambandam, Michael Steinmetz, Angel Chu, Judith Y. Altarejos, Jason R. B. Dyck
and Gary D. Lopaschuk
Department ... that levels of other mitochondrial-
Fig. 1. AMPK overexpression by adenoviral gene transfer and
endogenous MCD activity. (A) AMPK expression (i) and activity (ii) in
H9c2 ce...
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... glucose transpor-
ter gene (gcGLUT) and the corresponding full-length cDNA
from the grass carp. The gene spans 11 kb of genomic
sequence and consists of 12 exons and 11 introns, and an
open reading ... characteristics,
and regulatory properties [1,4] and may be attributed to a
different glucose requirement by various embryonic [5] and
adult [6] tissues.
Expression of the...