sp c gene structure and expression

... genomic DNA isolated from the insect C capitata as described previously [40] as template and the DS74 (5¢-TTG TGGAAAATCATACGAGA-3¢) and R1286 (5¢-CAAAC ACACATCGAGGAGC-3¢) oligonucleotides corresponding ... Journal compilation ª 2008 FEBS 6225 Genomic structure and expression analysis of the Cc RNase Expression and purification of Cc RNase recombinant protein The ORF of the Cc RNase cDNA 02 sequence was ... Journal compilation ª 2008 FEBS 6219 Genomic structure and expression analysis of the Cc RNase T N Rampias et al Fig Nucleotide and deduced amino acid sequences of the Cc RNase cDNAs The cDNA 01...

Ngày tải lên: 23/03/2014, 06:20

... http://www.ncbi.nlm.nih.gov /Structure/ cdd/cdd.shtml[81] h CC, coiled-coil motifs are predicted from the online COILS software http://www.ch.embnet.org/software/COILS_form.html[69] The two CCs in SUN5 are called CC1 and CC2 according to the ... first characterized in Schizosaccharomyces pombe and Caenorhabditis elegans as NE-associated proteins associated with spindle pole-body and nuclear-migration defects, respectively [24,25] Since ... around the nuclear periphery but also detected considerable speckled cytoplasmic staining in a postmeiotic uninucleate pollen mother cell The cytoplasmic staining may reflect nonspecific background...

Ngày tải lên: 11/08/2014, 11:21

... GCCCGTGATACAGGCGATCCACGATGCGCAGA GGTAGTAATGAG-3¢ and reverse 5¢-AATTCTCATTA CTACCTCTGCGCATCGTGGATCGCCTGTATCAC GGG-3¢ were used to construct the C- terminal fragment After phosphorylation and annealing, ... 5¢-GATCCATGTTTCTTCCGCTTCTGATCCTTGGCT CTCTTCTGATGAC-3¢ and reverse 5¢-CGGCGTCATCA GAAGAGAGCCAAGGATCAGAAGCGGAAGAAA CATG-3¢, were used to synthesize the N-terminal fragment of the poneratoxin gene Two others: forward 5¢-GCC GCCCGTGATACAGGCGATCCACGATGCGCAGA ... Synthetic oligonucleotides made with the codon choice suitable for AcMNPV [13] were used for the construction of the poneratoxin gene [11] Two oligonucleotides: forward 5¢-GATCCATGTTTCTTCCGCTTCTGATCCTTGGCT...

Ngày tải lên: 30/03/2014, 13:20

... indicate the actual time of the discharge Both high-voltage low-capacitance (hence short-time) and lowvoltage high-capacitance (hence long-time) conditions may be selected Special disposable cuvets ... Olson, L., and Schaffner, W (1983) A lymohocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes Cell 33, ‘727-740 Benoist, C and Chambon, ... promoter and the CD2 enhancer was expressed in both T-cells and erythroblasts (6) In conclusion, it seems likely that tissue specific gene expression can result from both promoter and enhancer elements...

Ngày tải lên: 11/04/2014, 09:42

... BamHl Xbal ACCTCGAGTGGCCACCATGGGCGAGCTCGGTACCCGGGGATCCTCTAGA Sstt +l Xhol Ball Ncol Kpnl EcoRl BamHl ACCTCGAGTGGCCACCATGGGCGAGCTCGGTACCCCCGAATTCGGGGGGATCCTCTAGA \ Xbal / 35s 42 kb CaMV poly A ... ACAGCCCAAGCATGGAGAACCGACCTGCAGGTCGACGGATCCCCGGGAATTC pJIT74 Sstl Kpnl 35s pJITI 14 CaMV poly A 39 kb +1 72 kb Hindlll ACAGCCCAAGCTTAACA +I EcoRl Hindlll Sal1 BamHl Smal EcoRl ATG GCG TGC AGG TCG ACG GAT CCC CGG GAA TTC Ncol Sal1 ... cassettewas able to direct the expression of the chloramphenicol acetyltransferase gene (cat,) in tobacco calli (4) 5 Genes for Transformation BamHl Smal ACAGCCCAAGCATGGAGAACCGACCTGCAGGTCGACGGATCCCCGGGAATTC...

Ngày tải lên: 11/04/2014, 10:10

... designated as 'contributing genes' We also calculated MCS density and intergenic TE frequency of contributing genes In particular, for intergenic sequences, TEna (igTEna) was calculated as described ... been carried out in parallel on human and mouse genes Such a procedure strengthens the ensuing conclusions since the majority of TEs are specific to either species [3] and the maintenance of ancestral ... between gene expression and GC-content in mammals: statistical significance and biological relevance Hum Mol Genet 2005, 14:421-427 Vinogradov AE: Compactness of human housekeeping genes: selection...

Ngày tải lên: 14/08/2014, 17:22

... GGACGTGAGCATGTATCCTAGCT CCTCGGCAGCCAAGTGAA TGATTCTACCCACGGCAAGT AACGACCCCTTCATTGAC TGCACCACCAACTGCTTAG GCCATTCACAGGGCACATTC CCTCCTGATGGTGATGTATCGA TAACCACCGTAGTCCGGGTACT AGCGACTGTAGTGAAACTCCTTCTC AGCATCACCCATTTGATGT ... TCCACGACATACTCAGCAC GACGCAGGGATGATGTTC CCGGTTCCTCTTGGTGTTCA 2756 FEBS Journal 272 (2005) 2746–2759 ª 2005 FEBS L Chen et al ogy Inc (Burlington, Ontario, Canada) per instruction Real time PCR ... the nonendocrine colon cancer cell lines Those data collectively suggested that activation of Cdx-2 expression by forskolin ⁄ IBMX occurs in a cell type speci c manner The InR1-G9 cell line may...

Ngày tải lên: 30/03/2014, 16:20

... gggaGGCGgggctt ttcatCCAAtca ctaaAATTacagtgt atcaatATGCtaata aacatatgTAATaata aggtAATTacaatga ttgattttAAATaaac ATAAtttttaaaca aATGCaaaaa aaatATTCaa cATGCcaatt aatCCAAtaac ccaCCAAtatc tcaCCAAttga ... ATATTGTCGTTAGAACGCGTAATACGACTCA-3¢; C2 : 5¢-CGTTAGAACGCGTAATACGACTCACTATA GGGAGA-3¢) First round PCR was performed using primer C1 and an external gene- speci c antisense primer (5¢-CTCGTCGAAGTTCTGGCTGTC-3¢; ... primers speci c to B-FABP mRNA (outer: 5¢-CACCAC CATCCATCATTGAC-3¢, nucleotides 2310–2291; inner: 5¢-CTCGTCGAAGTTCTGGCTGTC-3¢, nucleotides 127–107, Fig 1) The 10 lL reaction of the first round of PCR...

Ngày tải lên: 31/03/2014, 07:20

... ttaTAAAtcagccag ccCCCCcaggcc gtgaATCAa ggggGGCGgatgg cGTTAattagttttt tGATAataaatgtgaat ttaGATAaaa ccctGATAaatta tgctgGATAagtgg aatGATAata atgaCAATga tataCAATct atatggCCATttagtttatt catCCAAtcgc catCCAAtcac ... sequence and two antisense primers complementary to the 5¢ end sequence of cDNA (external: 5¢-TGCTCTCCTTCAAGTTCCA CG-3¢; internal: 5¢-AATGAGAGCGAGAGCAGATGG-3¢, Fig 1A) The amplified product was fractionated ... forward (5¢-ATCCAACTCCATCCACCTTC-3¢) and reverse (5¢-ATCAGGTTGTCAGTGTAGCC-3¢) primers [22] The RT-PCR conditions employed for detection of RACK1 mRNA were the same as RT-PCR of fabp3 mRNA (see...

Ngày tải lên: 31/03/2014, 07:20

... 5'-GTACGGGGATCCTTATCAGCACTCTTCCATCTCATCGAACTCCTG-3', F gene; 5'AAAAAAAAGGATCCACCATGGCACGAATCCTAAACCTCAAAGA-3', 5'-TTTCCCTGGGATCCTTATCACGCCGTCTTCCAGAACCCG-3' (initiation codon underlined) Preparation of other HCV protein expression ... 5'-TGCGGTACCGGCTAAATCGCAACTGCTTCCCCAG-3' (for IFN-λ1 promoter), 5'GCAACGCGTCATATTCCTGAGTCCTTCCTTGC-3' and 5'CCCGGTACCGTCTGTGTCACAGAGAGAAAGGGAG-3' (for IFN-λ3 promoter), 5'-ATGACGCGTGAAATTCAGGAGTAATCAGATC-3' ... pcDNA3.1(+)-FLAGtagged expression vector [43] Primers for NS3/4A; 5'AAGGGGGGATCCACCATGGCGCCCATCACGGCG- http://www.virologyj.com/content/3/1/66 TACGCCCAGCAG-3', 5'-GTACGGGGATCCTTATCAGCACTCTTCCATCTCATCGAACTCCTG-3',...

Ngày tải lên: 20/06/2014, 01:20

... modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats Journal of Biomedical Science 2010, 17:43 Page ... the beneficial effect of curcumin a neuroprotective agent, on impairment in dopaminergic receptors, CREB and phospholipase C in the cerebral cortex and cerebellum of STZ-induced diabetic rats Our ... Time-PCR Analysis of CREB in the cerebral cortex and cerebellum of control and experimental rats Real Time-PCR analysis showed that the CREB gene expression in the cerebral cortex and cerebellum...

Ngày tải lên: 10/08/2014, 05:21

... -ix/-ex -ices OBJECTIVE 3: CHECK FOR FULL SUBORDINATION A complex sentence contains at least two clauses: a main clause and a subordination clause A subordination clause can be ADJ, ADV or N clause ... how and can function in a sentence in any of the ways that a noun can A subordinate clause is a subject-verb combination that begins with a word such as because, which,if, and can’t function ... checklist OBJECTIVE 1: CHECK THE SUBJECT AND VERB Five types of structures which can function as subjects: noun, pronoun, gerund (V-ing), infinitive (to V ), noun clause Every sentence has at least...

Ngày tải lên: 13/12/2013, 03:15

... subject of the clause at the same time C Sentences with Reduced clauses Skill 13: Use reduced Adjective clauses correctly To reduce an adj clause: omit the adj clause connector/subject and the ... coordinate conjunctions (same structure) + (and, but, or) +(same structure) (same structure) , (same structure) , (and, but, or), (same structure) Skill 25: Use Parallel structure with paired conjunctions ... Use adj clause connectors/subjects correctly Adj clause Connectors/subjects: who/whom (for people), which (for things), that (both) Adj clause Connectors is not just a connector, it can also...

Ngày tải lên: 13/12/2013, 03:15

... answering correctly If you know an answer is wrong, you can eliminate that choice and increase your chance of answering correctly Structure and Written Expression: Grammar Tips 38 48 Structure: ... the verb, and "to the store" is the adverb clause telling where he went "Go!" is a complete sentence because it has a verb, "go" and "you" is the implied subject Command sentences such as this ... 35 Structure (page 84) Sentence Completion - 15 questions Error Identification - 25 questions Time: 25 minutes Tip 36 Sentence Completion For Sentence Completion you will be given a sentence...

Ngày tải lên: 24/12/2013, 19:15

... are classed as singular and you can replace them with "it" Countable nouns refer to things that can be counted, such as: cat, cats knife, knives child, children cookie, cookies can, cans, etc ... as", etc For example, "As good as he was, she was better" n Locate the main subject and a main verb n Remember that "because" usually signals a dependent clause which also contains a subject and ... eliminate choices that not supply both a subject and a verb for the sentence or that not agree with the rest of the sentence Error Identification Tips 51 - 59 Structure: Error Identification TIP...

Ngày tải lên: 24/12/2013, 19:15

... the plan; m Comply with rules NOT comply to rules; m In accordance with policy NOT in accordance to policy; m Jealous of others NOT jealous for others; etc l Other common idiomatic expressions ... weather/whether decent/descent complements/compliments principal/principle basis/ base except/accept These words may sound the same but have very different meanings Structure: Error Identification TIP ... each other, one is probably unnecessary (especially as an unnecessary second subject) for example, "The child he is very stubborn" should read, "The child is very stubborn." Structure: Error...

Ngày tải lên: 24/12/2013, 19:15

... Since recent (B) Because of recent (C) Recent (D) The result of recent 11 The terms of the Treaty of Versailles included that would effectively force Germany to compensate for the loss inflicted ... journalism and literature (C) (D) 17 Doctors now recommend that a balance in various activities, such as sleeping, working, eat, (A) (B) (C) (D) and socializing 18 Eleanor Roosevelt wrote her own specially ... ambulances can arrive at the scene of an accident within minutes of their (A) (B) occurrence, depending on the precision of the address description given to them by phone (C) (D) 38 After a 1980 recommendation...

Ngày tải lên: 26/12/2013, 00:17

... the principles which were to later affect the American constitution (A) While the characteristics of colonial values (B) Both colonial characteristics and their values For more material and information, ... Tai Lieu Du Hoc at www.tailieuduhoc.org (C) That the values of the colonies (D) The values of colonial characteristics 15 Speech is like a piece of cloth many threads including a voice box, lips, ... the chemical odor of female moths that they only need to pick up one molecule of the chemical to detect a female 33 A laser is the only device which aim coherent light at an object in a safe and...

Ngày tải lên: 26/12/2013, 00:17

... which can be mass-produced and, upon which, tens (A) (B) (C) of thousands of tiny discreet circuits can be wedged (D) 28 Heat is transferred in any of three ways, either of conduction, convection ... daytime sunlight can 13 White phosphorus, a substance in matches, is so flammable it burst into flame upon contact with the air (A) is common (B) common (C) which being common (D) being common, is ... more material and information, please visit Tai Lieu Du Hoc at www.tailieuduhoc.org (D) a technique of Black Boy is an autobiographical account of the childhood of Richard Wright and portrays...

Ngày tải lên: 23/01/2014, 06:20

... capitalized and financed if a A B C satisfactory conclusion is to be achieved D 19 The magazine article published an article on how much time was he wasting and A B included an analysis of his chance ... surgeries, once considered risky and dangerous, percent of recipients can now expect to live more than a year after the operation (A) which are now so successful (B) are now so successful (C) they ... Winston-Salem casino profits, (A) The Kootenay Band Tribe becoming (B) While The Kootenay Band Tribe becomes (C) Because The Kootenay Band Tribe became (D) The Kootenay Band Tribe has become There...

Ngày tải lên: 23/01/2014, 06:20