mrna processing and metabolism, methods and protocols
mrna processing and metabolism, methods and protocols
... Schoenberg
mRNA Processing
and Metabolism
Methods and Protocols
Volume 257
METHODS IN MOLECULAR BIOLOGY
TM
METHODS IN MOLECULAR BIOLOGY
TM
Edited by
Daniel R. Schoenberg
mRNA Processing
and Metabolism
Methods ... Biology, vol. 257: mRNA Processing and Metabolism
Edited by: D. R. Schoenberg © Humana Press Inc., Totowa, NJ
1
Using Chromatin Immunoprecipitation
to M...
telomeres and telomerase, methods and protocols
... PRESS
Methods in Molecular Biology
TM
VOLUME 191
Telomeres
and Telomerase
Methods and Protocols
Methods and Protocols
Edited by
John A. Double
Michael J. Thompson
Introduction to Telomeres and ... xi
Introduction to Telomeres and Telomerase 1
1
From:
Methods in Molecular Biology, vol. 191: Telomeres and Telomerase: Methods and Protocols
Edited by: J. A. Double...
hplc of peptides and proteins, methods and protocols
... Page 20
METHODS
IN
MOLECULAR
BIOLOGY
TM
Edited by
Marie-Isabel Aguilar
HPLC of Peptides
and Proteins
Volume 251
Methods and Protocols
Edited by
Marie-Isabel Aguilar
Methods and Protocols
HPLC ... Protocols
HPLC of Peptides
and Proteins
METHODS
IN
MOLECULAR
BIOLOGY
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9. Zhou, N. E., Mant, C. T., Kirkland J. J., and Hodges R. S. (1991) Comparison of
silica-bas...
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology
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HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
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HUMANA ... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins were mixed with DNA and...
Glycoprotein Methods and Protocols - P2
... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,
NJ.
50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
Glycoprotein Methods and Protocols - P3
... in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi-
cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The
M...
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple method...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... mucin mRNA level and the mucin precursor synthesis level, and
(2) the mucin precursor synthesis level and the total mucin levels (Note 11).
4. Notes
1. All calculations, apart from the mRNA quantitation, ... level...
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Human...
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolu...
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