membrane protein protocols

... Biology TM Edited by Barry S. Selinsky Membrane Protein Protocols VOLUME 228 Expression, Purification, and Characterization Edited by Barry S. Selinsky Membrane Protein Protocols Expression, Purification, and ... electron transfer protein that exists in a membrane- bound form in the endoplasmic reticulum where it is anchored to the membrane via a carboxyl-terminal transmembra...

Ngày tải lên: 08/04/2014, 12:51

... Biology TM Edited by Barry S. Selinsky Membrane Protein Protocols VOLUME 228 Expression, Purification, and Characterization Edited by Barry S. Selinsky Membrane Protein Protocols Expression, Purification, and ... electron transfer protein that exists in a membrane- bound form in the endoplasmic reticulum where it is anchored to the membrane via a carboxyl-terminal transmembra...

Ngày tải lên: 11/04/2014, 09:50

... [6,7], being mediated mainly by membrane proteins present on the bacillus and its host [8]. The M. tuberculosis cell membrane, and that of other prokaryotes, contains proteins func- tioning as enzymes, ... mansoni membrane- bound protein through bioinformatic analy- sis. Genet Mol Res 5, 609–618. 15 Kyte J & Doolittle RF (1982) A simple method for dis- playing the hydropathic chara...

Ngày tải lên: 18/02/2014, 16:20

... the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transiently or stably, with lamins, chromatin proteins and a vari- ety of regulatory proteins, including transcriptional regulators ... one of the well-defined integral INM proteins. Emerin is a type-II integral membrane protein of 34 kDa, which is inser- ted into the membrane with a single transmembr...

Ngày tải lên: 07/03/2014, 12:20

... topology of integral membrane proteins is nor- mally determined at the time of insertion into a target membrane. In both eukaryotic and prokaryotic cells, most membrane proteins are inserted ... positively charged residues in bacterial inner membrane proteins correlates with the trans -membrane topology. EMBO J 5, 3021–3027. M. Monne ´ et al. Kinetics of membrane protein insertion...

Ngày tải lên: 07/03/2014, 16:20

... precursor across the membrane [19]. Another subset of proteins is targeted post-translationally to the ER membrane by the TRC40–GET pathway. This subset of proteins comprises membrane proteins with ... cellular membranes [113]. Folding and assembly of multispanning membrane proteins Forces behind the folding of membrane proteins Next, we briefly introduce the molecular interactions d...

Ngày tải lên: 22/03/2014, 15:21

... MFG-E8; membrane secretion; exosome; per- ipheral membrane protein; milk fat globule membrane. MFG-E8 (milk fat globule-EGF factor 8) was cloned and characterized as mouse milk 53- and 66-kDa glycoproteins peripherally ... J. Biochem. 269) Ó FEBS 2002 Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion Ken...

Ngày tải lên: 24/03/2014, 03:21

... when handling micrograms of protein that are easily lost owing to unspecific binding to plastic tubes or dialysis/filtration membranes. 6. To microsequence a purified protein, two main techniques ... N-terminal sequence of the protein by Edman degradation, or to per- form N-terminal sequence of internal peptides of the protein, obtained after digestion with an endoproteinase (7). For N-...

Ngày tải lên: 10/04/2014, 22:13

... Molecular Biology TM Calcium-Binding Protein Protocols Edited by Hans J. Vogel VOLUME 173 Volume II Methods and Techniques Edited by Hans J. Vogel Calcium-Binding Protein Protocols Volume II Methods and ... Multifunctional S100 Protein Family Claus W. Heizmann 5Ca 2+ Binding to Proteins Containing γ-Carboxyglutamic Acid Residues Egon Persson 6The Caseins of Milk as Calcium-Binding P...

Ngày tải lên: 11/04/2014, 00:23

... I Reviews and Case Studies Calcium-Binding Protein Protocols Calcium-Binding Proteins 3 3 From: Methods in Molecular Biology , vol. 172: Calcium-Binding Protein Protocols, Vol. 1: Reviews and Case Studies Edited ... Ca 2+ -binding proteins, the protein kinases C (27). Protein kinases C bind Ca 2+ through their C2 domain calcium-binding motifs; it is now known that C2 domains exist...

Ngày tải lên: 11/04/2014, 00:24