peg on outer membrane protein x ompx

... hydrophobic length of a membrane protein and the hydrophobic thickness of the membrane it spans The common strategy for the functional reconstitution of membrane proteins into polymeric membranes requires ... Section Methods, “Engineering, expression and extraction of FhuAΔ1-159 Ext”) The nm increase of the hydrophobic protein portion is the limit to ensure the insertion of the FhuA Δ1-159 Ext into ... conducted on OmpF (outer membrane protein F) insertion into EO 29 EE 28 (Ethyleneoxide29-Ethylethylene28) membranes show a considerable symmetric deformation of the hydrophobic region of the polymer...

Ngày tải lên: 11/08/2014, 00:23

... Table S1) Extraction and Purification of FhuA Δ1-159 Exp Detergent extraction led to FhuA Δ1-159 Exp solubilized in buffer containing detergent oPOE (n-octylpolyoxyethylene) The sample contained ... flux is proportional to the diffusion coefficient and the concentration gradient As both variables were controlled by the experimental conditions maintaining the same values via the substrate concentration, ... Δ1-159 Exp”, Additional File 1) was subcloned into the E coli expression vector pBR-IBA1 (IBA, Göttingen, Germany) resulting in plasmid pBRIBA1 fhuA Δ1-159 exp Expression and Extraction of FhuA...

Ngày tải lên: 11/08/2014, 00:23

... temperature for h to ensure oxidation of the outer membrane cytochromes Absorption spectra of mL fractions were recorded at 554 nm using a double-beam spectrophotometer (Uvikon, Kontron, Herts, UK) in the ... on Fe(III) respiration by OmcB relative to OmcA can be explained by one or a combination FEBS Journal 274 (2007) 3728–3738 ª 2007 The Authors Journal compilation ª 2007 FEBS 3729 Shewanella oneidensis ... reflect the concentrations of OmcA and OmcB in omcB– and omcA– cells, respectively Maximal activities were converted to turnover numbers on the basis of either the OmcA or OmcB concentrations calculated...

Ngày tải lên: 07/03/2014, 09:20

... sau: * Nguồn gốc kinh tế - x hội tôn giáo - Do xh CSNT, trình độ phát triển LLSX thấp Th.sĩ Nguyễn Thị Thu Hà Trong XH CSNT, LLSX thấp kém, người chưa giải thích tượng x y giới có ảnh hưởng đến ... phá CNXH Th.sĩ Nguyễn Thị Thu Hà Trong công x y dựng CNXH, đồng bào có tín ngưỡng, tôn giáo phận quốc gia dân tộc Dưới CNXH, tôn giáo có khả tự biến đổi để thích nghi với tồn XH, theo xu hướng ... kiểu sinh hoạt văn hóa tinh thần thiếu sống Trong mối quan hệ tồn XH ý thức XH ý thức XH thường bảo thủ so với tồn XH, tôn giáo hình thái ý thức XH bảo thủ Do tồn lâu dài lịch sử nhân loại, tín...

Ngày tải lên: 17/08/2012, 22:02

... chuyển elêtron từ qỹu đạo M quỹ đạo K có bớc sóng = 0,1026m Hãy tính tính bớc sóng dài dãy Banme ? A 0,6566m B 0,6066 m C 0,6996m D 0,7016m Cõu 17: Cỏc vch bc x dóy Laiman quang ph vch phỏt x ca H ... kích thích cho elêtron chuyển lên quỹ đạo N nguyên tử phát vạch quang phổ dãy Banme ? Tính bớc sóng vạch ? A vạch 6566 B vạch 4869 C vạch 6566 4869 D vạch 4869 3956 Cõu 16: Trong quang phổ vạch ... cng dũng quang in bóo ho tng lờn gp ụi C ng nng ban u cc i ca cỏc eletron quang in tng gp ụi D tc ban u cc i ca cỏc eletron quang in tng gp ụi Cõu 20: Tỡm phỏt biu sai A Nu bỏn kớnh qu o K l R0...

Ngày tải lên: 29/07/2013, 01:26

... putative membrane protein [9] in the 6358 M tuberculosis genome, is present in M tuberculosis complex strain bacilli, is transcribed, expressed and present in greater concentration on the membraneenriched ... that HABP concentrations of less than 100 lm had a greater M tuberculosis Rv2560 protein characterization inhibitory effect than larger concentrations Therefore, additional CD secondary structural ... in NaCl ⁄ Pi The suspension was briefly sonicated (30 W for s) on a Branson sonifier (VWR Scientific, Boston, MA, USA); the suspension turbidity was adjusted to · 108 colony-forming unitsÆmL)1 according...

Ngày tải lên: 18/02/2014, 16:20

... integral membrane proteins in Triton X- 114 solution J Biol Chem 256, 1604–1607 24 Pryde JG & Phillips JH (1986) Fractionation of membrane proteins by temperature-induced phase separation in Triton X- 114 ... A B Results and Discussion Integral membrane proteins in the mitochondrial outer membrane The protein profile of mitochondrial outer membranes shows at least nine major proteins (Fig 1A, asterisks), ... alkali extraction Extraction of the membrane vesicles with alkali sodium carbonate releases several proteins that might be peripheral components of the membrane (Fig 1A) Under these conditions,...

Ngày tải lên: 19/02/2014, 07:20

... position of C-6 signals of uronic acids was found from HMBC correlations to H-5 protons Connections between monosaccharides were identified on the basis of NOESY (Fig 3) and HMBC correlations The ... and 3-amino-3,6-dideoxyhexose in a ratio of : 2.5 : : 1.6 : : 1.5, and additionally nonquan- tified glucuronic, galactosaminouronic, and galacturonic acids The presence of excess glucose (glucitol) ... at the injection Mass spectra were acquired with dwell times of 2.0 s per scan in positive ion detection mode Fragment ions formed by collision activation of selected precursor ions with nitrogen...

Ngày tải lên: 19/02/2014, 16:20

... adherence and invasion in vitro, colonization in vivo, maintenance of cell surface charge and serum bactericidal resistance [8–13] Which component on the outer membrane of M catarrhalis contributes to ... (2003) Human immune response to outer membrane protein CD of Moraxella catarrhalis in adults with chronic obstructive pulmonary disease Infect Immun 71, 1288– 1294 17 Pearson MM, Laurence CA, Guinn ... (1959) The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B I Identification J Biol Chem 234, 705–709 40 Murphy TF & Loeb MR (1989) Isolation of the outer membrane of Branhamella...

Ngày tải lên: 07/03/2014, 05:20

... phosphorylation state [20] Both samples, cut from the gel, contain more than one protein Also, it is not possible to exactly control the protein composition in such an excised gel piece One explanation ... Additional modifications Species Oxidation (M) Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Oxidation (M) Oxidation (M) 13 C6-Arg C6-Arg 13 C6-Arg and 13 13 13C6-Arg Oxidation ... compilation ª 2006 FEBS 3207 Tyrosine-phosphorylation of emerin A Schlosser et al mouse emerin, one Ser-phosphorylation site was detected on S-72 Tyrosine phosphorylation sites could only be detected...

Ngày tải lên: 07/03/2014, 12:20

... site (middle), or only the G2 site (bottom) The translation initiation inhibitor aurintricarboxylic acid was added to the translation mix 1.5 after addition of the mRNA, and Triton X- 100 was then ... molecules Discussion So far, not much is known about the kinetics of membrane protein insertion into the ER membrane and whether membrane protein topology is in some sense under kinetic control Studies ... transmembrane segment [7] Finally, the extracytoplasmic segments in the multispanning membrane protein bacterioopsin have been shown to become exposed on the extracellular surface cotranslationally...

Ngày tải lên: 07/03/2014, 16:20

... sides of the membrane imposes restrictions on the folding of the extramembrane regions, and, by extension, on the overall protein structure Concluding remarks Membrane protein integration appears ... chaperone for the integration of nonoptimal TM segments by providing a more favorable context [42] Translocon-associated proteins Some other membrane proteins [i.e translocon-associated protein ... et al Membrane protein integration translocating chain-associating membrane protein (TRAM) [22] As translocon activity can be reproduced by ab initio reconstitution of these four membrane proteins...

Ngày tải lên: 22/03/2014, 15:21

... masses Next we designed an experiment to demonstrate the gating function of the OprM channel In general the gate domain of channel proteins seems to have a flexible region, and the flexible regions ... T., Neshat, S., Yamagishi, J., Li, X. Z & Nishino, T (1996) Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa Mol Microbiol 21, ... Poole, K (2001) Mutational analysis of the OprM outer membrane component of the MexA-MexB-OprM multidrug efflux system of Pseudomonas aeruginosa J.Bacteriol 183, 12–27 22 Yoneyama, H., Ocaktan,...

Ngày tải lên: 23/03/2014, 21:21

... kmax and the intensity at 220 MPa or higher re¯ects changes in the lipid bilayer, in addition to protein conformational changes (see Discussion) Ó FEBS 2002 High pressure e€ects on biological membrane ... and FITC ¯uorescences were monitored at 330±560 nm with an excitation at 305 nm and at 500±560 nm with an excitation at 470 nm, respectively The sample solution contained 10 lg of BIPM- or FITC-labeled ... which formed on the inner wall of the test tube was mixed with mL of 50 mM Tris/HCl buffer, pH 7.5, by a Vortex mixer and subjected to ultrasonication RESULTS Effects of pressure on Na+/K+-ATPase,...

Ngày tải lên: 24/03/2014, 00:21

... not only on the cell surface but also in association with uncharacterized membrane vesicles secreted into culture medium The expression of several domain-deletion mutants of MFG-E8 suggests contribution ... biotinylation, confirming their existence on the cell surface In contrast, only weak or almost no bands were detected for the C-domain deletion mutants, indicating that they did not retain on the ... proteins secreted by COMMA-ID cells were completely precipitated under the ultracentrifugation condition used The precipitation by ultracentrifugation at 100 000 g and solubilization by Triton...

Ngày tải lên: 24/03/2014, 03:21

... Purification of proteins and construction of dimeric protein The OMPLA variants were overexpressed without their signal sequence resulting in the accumulation of inclusion bodies by induction with ... fraction of substrate in the assay as well as the concentration of Triton X- 100 (TX-100) was varied Substrate affinities (Km*) have been determined at both Triton X- 100 levels KCa (lM) [TX-100] ... surface-exposed The activation of OMPLA reconstituted in phospholipid vesicles was assessed by incubation at several calcium concentrations Degradation of phospholipids was followed on TLC and...

Ngày tải lên: 24/03/2014, 03:21

... experiments, the amount and the stability of the 70S ribosome in the K42T extract did not appear to differ from those of the wild-type extract Comparison of the optimum concentration conditions ... components, the reaction conditions, and the generation and the consumption of the energy source have been optimized in the cell-free system [42,43] For high-throughput protein production, the designed ... upstream of the expressed genes in expression vectors [44] There are no reports focusing on modification of the ribosome, the apparatus of translation, in terms of high-yield protein production Our development...

Ngày tải lên: 30/03/2014, 13:20

... they contained the marked phrases The matrix shown in Table defines three combinations of gold standard and system extraction results, A, B, and C: A B C Gold Standard extracted extracted not extracted ... the extraction performance We analyzed sentences from which the information was correctly extracted when only the GPD was used but erroneously extracted when the MLD+ was used There were six sentences ... semi-automatically Extraction Rules We manually defined extraction rules for active and passive sentences We converted the parsing output into XML format, and then applied the rules The following example...

Ngày tải lên: 31/03/2014, 20:20

... The construction of expression plasmids for the overexpression of three different Synechocystis Rieske proteins; Conditions for successful protein expression; and Characterization of the expressed ... the “colony size phenotype” on an IPTG plate is conserved 3.4 Optimization of Growth Conditions Optimization of expression conditions is essential for proteins that are difficult to express in ... inhibitor is highly toxic Heterologous Membrane Protein Expression 25 Methods 3.1 Designing Contructs for Expression As well as making expression constructs of the full-length protein, it may make...

Ngày tải lên: 08/04/2014, 12:51

... The construction of expression plasmids for the overexpression of three different Synechocystis Rieske proteins; Conditions for successful protein expression; and Characterization of the expressed ... the “colony size phenotype” on an IPTG plate is conserved 3.4 Optimization of Growth Conditions Optimization of expression conditions is essential for proteins that are difficult to express in ... Yeast extract peptone dextrose (YPD) medium containing 1% yeast extract, 2% peptone, and 2% dextrose YPD plates containing 1% yeast extract, 2% peptone, 2% dextrose, and 1% agar Regeneration dextrose...

Ngày tải lên: 11/04/2014, 09:50