another membrane bound transporter protein

... that the 80-kDa serine proteinase was a membrane- bound matriptase To verify that the 80 kDa proteinase has the IGFBP-rP1-cleaving activity, we separated the proteins in the membrane fraction of ... transmembrane serine proteinase [30] The matriptase subfamily constitutes three members (matriptase, matriptase and matriptase 3) It is conceivable that some of these membrane- bound serine proteinases ... matriptase MF, the membrane fraction before separation Other experimental conditions are described in ‘Experimental procedures’ (Fig 6B), indicating that this membrane- bound serine proteinase is the...

Ngày tải lên: 19/02/2014, 07:20

... and/or alternatively the release of free (nonprotein -bound) 125I from the cell Considering that 66–70% of 125I in the efflux medium was not proteinbound after labelling for 24 h with 125I-sMTf ... examination of the uptake of both the 59Fe label and the 125 I-labelled protein Protein-free 125 I assay The amount of protein- free 125I was measured in lysed cells and media using TCA precipitation ... separate internalized from membrane- bound 59Fe The results are a typical experiment from three performed and are expressed as the means of two determinations by the membrane than that internalized...

Ngày tải lên: 31/03/2014, 09:20

... membrane, and efficiently packaged into virions as they are released from the infected host cell Further, the membrane- bound immunomodulator must retain its bioactivity To ensure successful membrane ... http://www.virologyj.com/content/6/1/42 Figure Cell surface expression of membrane- bound immunomodulator fusion constructs Cell surface expression of membrane- bound immunomodulator fusion constructs Cell surface ... conjugated secondary antibody surface bioactivity of the specific membrane- bound cytokines as further described below Membrane- bound cytokine bioactivity was determined using specific cell-based...

Ngày tải lên: 12/08/2014, 04:21

... and yielded nucleosolic proteins The remaining pellet contains all DNA and structure bound proteins and is further referred to as chromatin-fraction Electrophoresis of proteins and Western blotting ... succession Separating a cell fraction containing DNA bound proteins Entire replicative SV40 minichromosomes bearing functionally bound replication proteins can be eluted from nuclei of virus infected ... relations and extracting unbound replication proteins from the nuclei seemed to be more appropriate for studying the influence of oxygen recovery after a hypoxic period on DNA -bound proteins For this purpose,...

Ngày tải lên: 21/02/2014, 00:20

... homogenizer Protein was solubilized from the membranes with 15 mM dodecyl-b-Dmaltoside [2 mg dodecyl-b-D-maltosideÆ(mg protein) )1] at °C for 12 h The unsolubilized proteins and the membranes were ... the membrane- bound enzyme from M barkeri [4,10], which is anchored in the cytoplasmic membrane via a btype cytochrome [3] We used this knowledge to identify and purify heme-containing membrane- bound ... HmcC, has 10 membrane- spanning helices These three proteins are also related to the DmsC protein of dimethylsulfoxide reductase [29] The latter protein contains only eight predicted transmembrane...

Ngày tải lên: 21/02/2014, 03:20

... fusion proteins Condensation assay of (His)6-FAE1 KCS (His)6 fusion proteins were solubilized and isolated as described [20] Microsomes (2 lg protein lL)1) expressing (His)6-tagged fusion proteins ... substrates are expected to be delivered from the cytosol and available at the membrane interface [1] This is in contrast to membrane- bound fatty acid- and sphingolipid-desaturases, which appear to utilize ... studies represent the first steps toward a characterization of the substratebinding site of the membrane- bound KCS enzymes Ó FEBS 2002 sequenced by in-house automated DNA sequencing to ensure they...

Ngày tải lên: 21/02/2014, 15:20

... antibody against POP Membrane PE, lg of protein; PPP, 120 ng of protein (B, C) Blot on total membranes (TM), light-density membranes (LM), mediumdensity membranes (MM) and high-density membranes (HM) ... would link the protein to the intermembrane milieu To further confirm this, we ran a temperature dependence assay with membrane PE-containing membranes (Fig 7) Intrinsic membrane proteins display ... western blotting (lower panels) TM, total membranes; LM, low-density membranes; MM, medium-density membranes; HM, high-density membranes The amounts of protein loaded onto gels were as follows:...

Ngày tải lên: 07/03/2014, 06:20

... However, for these proteins, one cannot determine from these data which domain bound first to the membrane: T, C, or both These results indicated that isolated C bound the membrane at about one ... conformations, in solution, membrane- bound, and membrane- inserted, stabilizes partially unfolded states of the C domain In doing so, the T domain favors membrane binding and membrane penetration of ... are responsible for its membrane binding and penetration into the lipid bilayer Membrane interaction of diphtheria toxin Results Recombinant proteins Five recombinant proteins were used in this...

Ngày tải lên: 14/03/2014, 22:20

... midgut membranes Results Solubilization of A pisum membrane- bound midgut APN About 98% of APN midgut activity [l-leucine-p-nitroanilide (LeupNA) as substrate] was found to be membrane- bound The ... X-100-released proteins from midgut cell membranes; lane 3, Mono Q eluate (purified aminopeptidase) (C) Glycoprotein detection (Dig Glycan detection kit), after western blots of proteins Lane 4, ... molecular species of A pisum membrane- bound aminopeptidase in this tissue, as judged by Mono Q chromatography after solubilization of almost 100% of its activity The membrane- bound APN from A pisum...

Ngày tải lên: 16/03/2014, 12:20

... solubilized extract of HUVECs membranes was efficient, because the membrane extract had the specific signals for each membrane protein The extract of solubilized membranes of HUVECs showed several ... solubilized membranes of HUVECS (30 lg of protein per lane), Human recombinant AChE standard (4.5 lg of protein per lL of sample) and human erythrocyte AChE standard (0.06 lg of protein per lL ... solubilized membranes of HUVECS (30 lg of protein per lane), human recombinant AChE standard (0.06 lg of protein per lL of sample) and human erythrocyte AChE standard (4.5 lg of protein per lL...

Ngày tải lên: 16/03/2014, 23:20

... [3Fe)4S] cluster EPR spectra of membranes of M barkeri As Ech (a membrane- bound protein) constitutes up to 3% of the total cell protein, we have also inspected membranes of M barkeri with EPR ... The best way to study membrane- bound enzymes, especially for those expected to pump protons, is to use intact membranes As demonstrated, % 3% of the protein content of membrane preparations of ... concentration was at least 2.1 g pure enzyme protein L)1 Estimating the total protein concentration to be % 80 gÆL)1, this is about 2.7% of the total membrane protein Because in some cases redox dyes...

Ngày tải lên: 17/03/2014, 10:20

... ubiquitinated form of the membrane- bound form of nitric oxide synthase on SDS/PAGE [43] bTHmem behaves as an integral membrane protein [7,9], but the mechanism by which it is bound is not yet resolved ... found to be integral membrane proteins including some high-molecular-mass glycoproteins [46] As the synaptic membrane fraction represents a mixture of different types of membranes, the functional ... immunoisolation the beads were mixed with samples of the membrane proteins and rocked in Eppendorf tubes for h at °C The protein A–Sepharose with bound IgG–TH was pelleted by centrifugation at 12 000...

Ngày tải lên: 17/03/2014, 17:20

... binds to cell membranes via multiple receptors Our data also suggest that the manner of annexinin II binding to cell membranes varies considerably from one cell type to another Membrane- bound annexin ... matrilysin, several proteins, including a major protein of approximately 35 kDa, were released from the membrane fraction (Fig 1A) The N-terminal amino acid sequence of the 35 kDa protein was determined ... analyzed membrane proteins that are cleaved by matrilysin A membrane fraction of WiDr human colon carcinoma cells was prepared by the phase separation method with Triton X-114 When the membrane...

Ngày tải lên: 17/03/2014, 17:20

... POPC and raft membranes have a very similar structure and demonstrate that the structure of PrP in these membranes resembles the structure of anchorless protein in solution Discussion Membrane- anchored ... plane of the cell membrane Some of these orientations would allow the possibility of direct interactions of the protein with the membrane surface, which could lead to a different protein structure ... into membranes PrP–GPIm was anchored in lipid membranes through the insertion of the hydrocarbon chains of GPIm into the lipid bilayer Several methods are commonly used to reconstitute integral membrane...

Ngày tải lên: 23/03/2014, 10:21

... Peroxisomal membrane protein import Most mutants that are defective for the import of PTS1 and PTS2 proteins still import peroxisomal membrane proteins Thus, the import of peroxisomal membrane and ... peroxisomal membrane and matrix proteins is independent [41,42,72] The peroxisomal membrane protein targeting signals (mPTS) were identified for several peroxisomal membrane proteins (PMPs) These targeting ... PMP-specific chaperone Pex3p – anchor protein for Pex19p at the peroxisomal membrane Pex3p is a peroxisomal membrane protein that interacts with Pex19p at the peroxisomal membrane [96] The N-terminal...

Ngày tải lên: 23/03/2014, 13:20

... blot PP17 antigen (1 ng) and 10–10 lg protein from term placenta total protein extract, HeLa total protein extracts and subcellular fractions, milk total protein extracts and MLMG fractions were ... pH 7.4 containing 150 mM NaCl to remove unbound proteins The immunoreactive proteins were removed with an equal volume of · Laemmli sample buffer, then proteins were separated by gradient (6–18%) ... as for proteins Immunoaffinity purification and protein identification by MS Anti-PP17 Ig was coupled to CnBr-activated agarose beads and incubated with lipid loaded HeLa cell or milk total protein...

Ngày tải lên: 23/03/2014, 21:20

... transmembrane proteins in the contact surfaces of proteins and water, and/or protein and lipid bilayer: an increase in the thickness of the lipid bilayer partially covers the transmembrane proteins ... incorporation into membrane- bound Na+/K+-ATPase Membrane- bound Na+/K+-ATPase was mixed with tritiated water at various pressures and at 37 °C for 15 and ®ltered by Millipore ®lter membranes After ... Ó FEBS 2002 High pressure e€ects on biological membrane (Eur J Biochem 269) 111 the cell membrane consisting of lipid bilayer and transmembrane proteins EXPERIMENTAL PROCEDURES Materials ATP...

Ngày tải lên: 24/03/2014, 00:21

... is bound to Tyr186 and Arg483 4732 J Simunic et al In addition to these features common to most ACEs, Asl-tACE displays some more specific ones Especially, it appears to be a membrane- bound protein ... to determine whether or not every isoform displays a transmembrane region Knowing whether all isoforms in Astacus are membrane bound proteins is interesting because it raises questions about ... speculate whether, in crustaceans, in contrast to other groups, ACE isoforms are always membranebound proteins because no genome has been sequenced from a crustacean species other than Daphnia...

Ngày tải lên: 30/03/2014, 01:20

... chromatography of membrane proteins The leaderless form of MltA was expressed, purified and covalently bound to CNBr-activated Sepharose This column was used to isolate MltA-interacting proteins from a membrane ... recombinant proteins Recombinant MltA lipoprotein was purified from the membrane fraction of E coli as described in Experimental Procedures Analysis of the purification by SDS/ PAGE showed that MltA lipoprotein ... overnight, membrane debris was removed by centrifugation (100 000 g for 45 min), and the supernatant containing solubilized protein stored at )20 °C Penicillin-binding assay Penicillin-binding proteins...

Ngày tải lên: 31/03/2014, 23:20

... http://www.ncbi.nlm.nih.gov /protein/ 15223067], At3-MMP [GenBank: NP_173824 http://www.ncbi.nlm.nih.gov /protein/ 30688744], At4-MMP [GenBank: NP_182030 http://www.ncbi.nlm.nih.gov/ protein/ 15225398], At5-MMP ... http://www.ncbi.nlm.nih.gov /protein/ 15218963], SMEP1 GenBank: P29136 http://www.ncbi.nlm.nih.gov /protein/ 2827777], GmMMP2 [GenBank:AAL27029 http://www.ncbi.nlm.nih.gov /protein/ 16901508], Cs1-MMP ... the cysteine switch motif Another key feature is that NtMMP1 contains a second cysteine residue (Cys 50) in the N-terminal portion of the protein According to the Scratch protein predictor server...

Ngày tải lên: 12/08/2014, 03:20