in situ hybridization protocols, 2nd

... Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppres- sion hybridization using recombinant DNA libraries. Hum. Genet. 80, 224–234. 3. Collins, C., Lin ... combinations of protein markers on the chromosome and DNA probes have been used to investigate the meiotic chromosomal pairing pro- cess; the role of core protein in homologous chromatin...

... trong vi sinh vật học là fluorescein-derivates (Fluorescein- Isothiocyanate (FITC), 5(6)carboxyfluorescein-N-hydroxysuccimide-ester (FluoX)) và rhodamine-derivates (Tetramethyl-Rhodamine-Isothiocyanate ... cộng sinh Hầu hết các vi sinh vật cộng sinh bắt buộc đều chưa tiến hành nuôi cấy được. Sử dụng rRNA 16S, chúng có thể được định danh và phân cấp phát sinh loài. Việc khoanh vùng vi si...

... Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis: Versatile Strategy to Move Beyond Structural Limitations of 20 Natural Amino Acids Side Chains in Protein Engineering Grace ... Single-Stranded Uracil-Substituted Template Single-stranded uracil containing phagemid DNA, containing the insert of interest, was prepared from E. coli RZ1032, using established procedu...

... Mougin et al. 28. Embleton, M., Gorochov, G., Jones, P., and Winter, G. (1992) In- cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within ... Air-dry Embed in a protein matrixC Time Temperature 30 min 37°C 5 min 37OC 10 min 30 mm 30 min Room temperature 37Y! 37°C 5-10 min 37OC 1-2 min 37°C 1s Room temperature 10 min...

... transferring to TB buffer for 15 min. Rinse 3× for 4 min each time in PBS. 6. Block for 10 min in PBS + 2% BSA. Rinse 3× for 4 min each time in PBS. 7. Incubate with fluorochrome-linked streptavidin ... avoid trapping air bubbles which may lead to staining artifacts. 8. Stop reaction by incubating slides 2 × 10 min in 2×SSC. 9. Rinse slides in PBS then block nonspecific binding by c...

... In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... sử dụng avidin (hay streptavidin) – biotin: đây là một cặp ligand có hằng số ái lực cao nhất tìm thấy trong sinh học. Đầu tiên, mẫu dò đƣợc đánh dấu bằng biotin theo hai cách: biotin đƣợc g...