in situ rock stress

Báo cáo khoa học: A ﬂuorescence energy transfer-based mechanical stress sensor for speciﬁc proteins in situ pdf

... adding and delet-ing amino acids. Positive symbols indicate an increasing amount,and negative symbol indicate a decreasing amount.No. amino acidsadded or subtractedChange in lengthof linker ... struc-tural proteins such as collagen-19 (COL-19), nonery-throcyte spectrin, a-actinin and ﬁlamin A withinliving cells, and that the FRET from this cassettechanges with stress in situ. The efﬁciency ... proteins, inclu-ding COL-19 (Fig. 10G), nonerythrocyte spectrin(Fig. 10E), ﬁlamin A (Fig. 10C), and a -actinin(Fig. 10A), and expression systems including HEK-293,3T3 and C. elegans, and the insertion...

Tìm hiểu kỹ thuật lai huỳnh quang tại chỗ FISH (Florescence In Situ Hybridization) trên vi sinh vật

Danh mục: Sinh học

... colonization of large intestines of streptomycin-treated mice. Infect. Immun. 67, 6152–6156. [37] Felske, A., 1998. In situ detection of an uncultured predominant Bacillus in dutch grassland ... trong vi sinh vật học là fluorescein-derivates (Fluorescein-Isothiocyanate (FITC), 5(6)carboxyfluorescein-N-hydroxysuccimide-ester (FluoX)) và rhodamine-derivates (Tetramethyl-Rhodamine-Isothiocyanate ... in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. Infect. Immun. 62, 673–679. [40] Forsgren, J., 1996. Persistence of nontypeable Haemophilus influenzae...

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Ứng dụng phương pháp in Situ hybridiza để chẩn đoán mầm bệnh WSSV trên tôm sú

Danh mục: Sinh học

... sử dụng avidin (hay streptavidin) – biotin: đây là một cặp ligand có hằng số ái lực cao nhất tìm thấy trong sinh học. Đầu tiên, mẫu dò đƣợc đánh dấu bằng biotin theo hai cách: biotin đƣợc gắn ... với dung dịch Blocking buffer 1X, chất blocking trong dung dịch sẽ khóa các phân tử lai không đặc hiệu lại.  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase ... DỤC VÀ ĐÀO TẠO ĐẠI HỌC NÔNG LÂM TP. HỒ CHÍ MINH BỘ MÔN CÔNG NGHỆ SINH HỌC  KHOÁ LUẬN TỐT NGHIỆP ỨNG DỤNG PHƢƠNG PHÁP IN SITU HYBRIDIZATION ĐỂ CHẨN ĐOÁN MẦM BỆNH WSSV...

ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS

Danh mục: Môi trường

... the combined study, we will illustrate the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria on fine scale in autotrophic nitrifying biofilms by applying the full-cycle ... allows relating in situ microbial activity directly to occurrence of nitrifying bacteria population. KEYWORDS : Nitrifying biofilms, 16S rDNA-cloning analysis, fluorescent in situ hybridization ... performed. In situ hybridization of vertical biofilm thin sections clearly indicated that the numbers of probe NEU-stained NH4+-oxidizing bacteria (i.e., Nitrosomonas marina-lineage, Nitrosomonas...

Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt

Danh mục: Báo cáo khoa học

... substrates for the enzymesinvolved in metabolism of acetylenic compounds, resulting in products that are suitable for bacterial growth. Weisolated a Pseudomonas putida strain growing on2-butynedioate ... found in more naturalproducts than bromides, chlorides, or nitriles [1]. Com-pounds containing triple bonds are widely distributed in plants, fungi, bacteria, and other living organisms [1]. In addition ... putida strain growing on 2-butyne-dioate or propynoate as sole carbon source. To investigatethe acetylene bond biodegradation, we used in situ protonnuclear magnetic resonance (1H-NMR) in water...

micelle-assisted synthesis of polyaniline magnetite nanorods by in situ self - assembly process

Danh mục: Vật lý

... Zhang).1Also working in College of Chemistry, Jilin University, Changchun 130021,PR China.a multitude of applications, such as in gas sensitivity, sensors,and inductors [11–13]. In recent years, ... been increas-ing interest in the synthesis of conductive organic/inorganicnanocomposites. A few of works had been reported on thesynthesis PANI/inorganic nanocomposites [14–16]. Hopkinset ... College of Science, Beijing Institute of Technology, Beijing 100081, PR ChinaReceived 21 September 2007; accepted 5 January 2008Available online 10 January 2008AbstractPolyaniline/magnetite nanocomposites...

BẢO TỒN IN SITU TÀI NGUYÊN DI TRUYỀN CÂY TRỒNG Ở VIỆT NAM, THỰC TRẠNG VÀ GIẢI PHÁP docx

Danh mục: Nông nghiệp

... Hodgkin T., 2000. A training guide for in situ conservation on farm. Version 1. Rome, International Plant Genetic Resources Institute. 7. Quy T. D and Hue N.T.N (editors), 2006. In situ Conservation ... is maintained and sustained operation. In this context, the appreciation of the actual status, indicate the limitations and reasons, which propose the approaches to promote conservation insitu ... yet promoted insitu conservation of crop genetic resources in our country. In the paper, some policy, administrative and technical approaches are also proposed. Keywords: In situ conservation,...

Báo cáo khoa học: Identiﬁcation of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf

Danh mục: Báo cáo khoa học

... believed to act cooperatively in the cross-linkingof proteins, including involucrin, loricrin and smallproline-rich proteins (SPRs). Such concerted reactionsresult in formation of the corniﬁed ... detecting in situ activ-ity of TGase 3 in a frozen mouse skin section.As shown in Fig. 6, in the presence of CaCl2, speciﬁcincorporation of FITC–pepE51 (1 lm) in endogenousglutamine-acceptor ... consisting of covalent cross-links of proteins beneath the plasma membrane ofterminally differentiated keratinocytes [23,24]. Further-more, TGase 3 in hair follicles is involved in cross-linking...

NANOSENSORS AS RESERVOIR ENGINEERINGTOOLS TO MAP IN- SITU TEMPERATURE DISTRIBUTIONS IN GEOTHERMAL RESERVOIRS doc

Danh mục: Điện - Điện tử

... a field study in which a conservative tracer (fluorescein) and a reactive tracer (rhodamine WT) were injected into the Steamboat Hills reservoir. The decay kinetics of rhodamine WT were extrapolated ... very far into the reservoir. Initial investigations were performed to evaluate the suitability of tin-bismuth alloy nanoparticles as nanosensors with a sensing mechanism of size increase induced ... induced by melting. Tin-bismuth was chosen as a candidate for investigation because its melting point can be tuned across a wide range of temperatures that are suitable for sensing in geothermal...

in situ detection of dna damage, methods and protocols

Danh mục: Sinh học

... aspirate buffer.3. Incubate while rocking for 1 h at room temperature in the dark in 100 µL ofstaining buffer containing 10 µg/mL of FITC-conjugated streptavidin. Wash bydiluting the sample with ... colabeling of DNA damage and specific cellular proteins.2. Development of In Situ DNA Labeling2.1. Characterization of DNA Polymerase Activitiesand the Invention of In Situ DNA Labeling In ... with 5 µg/mL proteinase K in PBS for 30 min and wash in PB for 30 min.4. Postfix the treated specimens with 0.25% GL in PB for 30 min and wash in PBfor 10 min.5. Immerse them in distilled water...

in situ hybridization protocols, 2nd

Danh mục: Sinh học

... chromosomes is described in Subheading 3.9.4.).Carry out all dipping and washing steps in Coplin jars or slide tanks.1. Denature the DNA in situ by dipping the slides in 0.07 M NaOH (made from ... wash solution C 3× for 3 min each.6. Stain with DAPI by immersing the slides in 0.2 µg/mL of DAPI in 2× SSC for 5–10 min at room temperature. Rinse in 2× SSC 3× for 2 min each.7. Mount the slides ... the slides in 2× SSC solution for 2 min.3. Dehydrate the slides by incubating for 3 min in 75%, 90%, and 100% ethanol andair dry.Human Partial Chromosome Paints 79. Vortex.10. Coplin jar.11....

prins and in situ pcr protocols

Danh mục: Sinh học

... after rinsing in distilled water. 6. In the case of labeling with biotin-16-dUTP or fluorescein-12-dUTP, a fourfold decrease in the concentration of dTTP in the PRINS reaction mix resulted in ... for 2 x 5 min in washing buffer. Alternatively, you may wash the slides for 5 min in PBS and dehydrate them. 13. Mount the slides in Vectashield containing 0.5 pg/mL DAPI. 14. Examine the slides ... Air-dry Embed in a protein matrixC Time Temperature 30 min 37°C 5 min 37OC 10 min 30 mm 30 min Room temperature 37Y! 37°C 5-10 min 37OC 1-2 min 37°C 1s Room temperature 10 min Room temperature...

XỬ LÝ Ô NHIỄM TẠI CHỖ (IN-SITU)

Danh mục: Môi trường

... LÝ Ô NHIỄM TẠI CHỖ (IN- SITU) 3.1. Xử lý đất chưa bão hòa (Bioventing) • Kỹ thuật xử lý sinh học duy nhất có thể áp dụng chođất chưa bão hòa là kỹ thuật cấp khí (Bioventing).• Nguyên tắc: ... bơm hút Bể trộn dinh dưỡngBơm đẩyMực nướcĐưa thêm dinh dưỡngXỬ LÝ Ô NHIỄM NƯỚC NGẦM: Kỹ thuật bơm dinh dưỡng (Bioenhancement)• Kỹ thuật chiết hút chân không (bioslurping) hay (vacuum-enhanced ... sinh họcvà quy trình công nghệCÔNG NGHỆ SINH HỌC MÔI TRƯỜNGTHS: VƯU NGỌC DUNG• Mục tiêu: khoáng hóa các chất ô nhiễm bằng các vi sinh vật để tạo các sản phẩm không gây hại.• Các vi sinh...