gene transfer and expression protocols

gene transfer and expression protocols

... Methods in Molecular Biology, Vol. 7: Gene Transfer and Expression Protocols Edited by: E J Murray 0 1991 The Humana Press Inc., Clifton, NJ 15 16 Okayama and Chen The calcium phosphate method ... chloramphenicol acetyl transferase (CAT) gene. This type of gene transfer is often referred to as a transient expression system. Production of the CAT enzyme peaks at around...

Ngày tải lên : 11/04/2014, 09:42

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plant gene transfer and expression protocols

... hygromycm B phosphotransferase gene and its expression in Escherzchza colz and Saccharomyces cerevhae. Gene 25, 179-l 88 34. Jones,’ J. D. G., Svab, Z , Harper, E. C., Hurwitz, C D , and Mahga, P. ... more and more diverse tools for gene transfer and expression. In this chapter, I will give a list of plasmid constructs containing various components use- ful for express...

Ngày tải lên : 11/04/2014, 10:10

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gene isolation and mapping protocols

... subsequent genettc linkage analysis, and the calculation of gene frequencies. The ordering of several markers, if unknown, into a genetic map, and the calculation of recombination fractions and genetic ... Soares, M B , and Sikela, J M (1995) Gene- based sequence-tagged-sites (STSs) as the basis for a human gene map. Nat Genet 10, 415-423. Linkage Analysis of Genetic Disorder...

Ngày tải lên : 11/04/2014, 09:42

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vascular disease, molecular biology and gene transfer protocols

... Biology and Gene Therapy Protocols Edited by: A. H. Baker © Humana Press Inc., Totowa, NJ 2 Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis Ana Cenarro, Fernando ... methodologies and limitations, in Genetics of Common Dis- eases (Day, I. N. M. and Humphries, S. E., eds.), BIOS Scientific Publishers, Oxford, pp. 33–74. 2. Cooper, D. N. and Krawcz...

Ngày tải lên : 11/04/2014, 10:39

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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt

... plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations, temperature and time of induction ... and Richard A. Houghten (Editors), American Peptide Society, 2001, pp. 387-388. 2. Bozenna Rempola, T-Wilusz, W. Markiewicz and M.Fkus, Synthesis, cloning and expression in E.col...

Ngày tải lên : 12/02/2014, 10:20

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Báo cáo khoa học: Cloning and expression of the ﬁrst nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

... prostaglandin E2, IL-10 and IL-11 generation in antigen presenting cells. Clin Exp Immunol 129, 438–445. 7 Kernacki KA, Goebel DJ, Poosch MS & Hazlett LD (1998) Early cytokine and chemokine gene ... decidua and fetoplacental development in mice. Genes Dev 12, 2234–2243. 22 Wang T & Secombes CJ (2003) Complete sequencing and expression of three complement components, C1r, C...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf

... receptor gene expression cassettes (DBD:EcR and AD:RXR), whereas the monopartite gene switch requires one receptor gene expression cassette (AD:DBD:EcR), to transactivate the reporter gene expression ... VP16:CH9 (VCH9) and luciferase expression cassettes to generate a two-hybrid gene switch (p2300GCfE VY :VCH9:Luc) and VGCfE VY and luciferase expression cassette...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx

... H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique Nandakumar Sambandam, Michael Steinmetz, Angel Chu, Judith Y. Altarejos, Jason R. B. Dyck and Gary D. Lopaschuk Department ... that levels of other mitochondrial- Fig. 1. AMPK overexpression by adenoviral gene transfer and endogenous MCD activity. (A) AMPK expression (i) and activity (ii) in H9c2 ce...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

... glucose transporter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans  11 kb of genomic sequence and consists of 12 exons and 11 introns, and an open reading ... characteristics, and regulatory properties [1,4] and may be attributed to a different glucose requirement by various embryonic [5] and adult [6] tissues. Expression of the...

Ngày tải lên : 17/03/2014, 03:20

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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... expression profile of the RNase j gene at various developmental stages and in several tissues of the insect C. capitata. We have also determined the general genomic organization of this gene and ... of these have been purified and characterized as alkaline [12] and acidic RNases [13], and a third poly(U)- and poly(C)-specific ribo- nuclease has also been isolated and characte...

Ngày tải lên : 23/03/2014, 06:20

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