dna repair protocols, prokaryotic systems
dna repair protocols, prokaryotic systems
... Molecular Biology
TM
Edited by
Pat Vaughan
DNA Repair
Protocols
VOLUME 152
Prokaryotic Systems
DNA Repair
Protocols
Prokaryotic Systems
Edited by
Pat Vaughan
Repair of Oxidative Damage 21
4. Proteinase ... single DNA base mutations with
mismatch repair enzymes. Genomics 14, 249–255.
26. Hsu, I C., Yang, Q. P., Kahng, Y. W., and Xu, J F. (1994) Detection of DNA
point mu...
dna repair protocols, eukaryotic systems
... involved in DNA repair, a
32
P-labeled DNA
probe is prepared so that it contains the DNA lesion or DNA structure of inter-
est. To mask the effects of nonspecific DNA binding proteins, the DNA probe
is ... Non-
checkpoint DNA repair mutants, when incubated with DNA- damaging agents
die with a highly elongated phenotype, because they are unable to repair the DNA
damage....
Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... transferase.
Keywords: deoxyribose phosphate lyase; DNA polymerase;
DNA repair enzyme; terminal deoxyribonucleotidyl trans-
ferase.
Ten years ago, only five DNA polymerase species (a to e)
were known, ... elucidation of
mechanisms of DNA repair in the cells in which DNA or
does or does not replicate.
We report here the characterization of Pol k from a
higher plant, rice, using cD...
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... Center for the Repair System of Damaged DNA, Korea Advanced
Institute of Science and Technology, South Korea
2 Korea Basic Science Institute, Daejon, South Korea
Nucleotide excision repair (NER) ... 2467–2476 ª 2005 FEBS
Solution structure and backbone dynamics of the
XPC-binding domain of the human DNA repair protein
hHR23B
Byoungkook Kim
1,
*, Kyoung-Seok Ryu
2,
*, Hyun-Jin Kim
1
,...
Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... various DNA repair mechanisms, the
repair of a specific DNA damage is often conserved from
bacteria to human, and in many cases the proteins are
highly similar [5]. Little is known about DNA repair
mechanisms ... gondii DNA repair enzyme;
RRM, RNA recognition motif; HCA, hydrophobic cluster analysis;
MMC, DNA- damaging agent mitomycin C; G-patch, glycine rich
motif; SF45, spli...
Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... inactivate DNA
repair processes by t he oxidation of zinc finger structures in
DNA repair proteins. As these observations are derived
from subcellular systems, experiments on interactions with
DNA repair ... involved in DNA damage signaling and
repair, such as poly(ADP-ribose) polymerase-1, formami-
dopyrimidine -DNA glycosylase (Fpg), which is involved in
the repair of cert...
Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
... of DNA during replication, repair and transcrip-
tion processes by making single stranded breaks on DNA,
unwinding and religating the DNA ends in the cleaved
strand [1]. During the process, DNA ... in
carrying out DNA religation [3]. Consequently, a normally
transient break in DNA could become a long-lived double-
stranded break upon collision of Topo I DNA complex
and DNA re...
dna methylation protocols
... using DNase I to nick the DNA and the DNA polymerase I
holoenzyme to label the DNA by nick translation gives less reproducible results
than the fi ll-in method using Klenow fragment of DNA polymerase ... RNA. Wash the DNA pellet with 70%
ethanol and resuspend in 100 µL deoxyribonuclease I (DNase I) digestion
buffer.
3.2. DNA Hydrolysis
1. Follow steps 1–3, Subheading 3.1.
2....
dna topoisomerase protocols, volume i
... 5,6).
1.1. DNA Shape
Circular plasmid DNA can exist in a number of different topological confor-
mations. Superhelical circular DNA (form I), nicked circular DNA (form II),
and linear DNA (form ... supercoiled
DNA topoisomers migrate faster through agarose in comparison to linear DNA,
nicked circular DNA, or relaxed DNA. For example, as shown in Fig. 1, nega-
tively supercoile...
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