dna repair protocols, prokaryotic systems

... Molecular Biology TM Edited by Pat Vaughan DNA Repair Protocols VOLUME 152 Prokaryotic Systems DNA Repair Protocols Prokaryotic Systems Edited by Pat Vaughan Repair of Oxidative Damage 21 4. Proteinase ... single DNA base mutations with mismatch repair enzymes. Genomics 14, 249–255. 26. Hsu, I C., Yang, Q. P., Kahng, Y. W., and Xu, J F. (1994) Detection of DNA point mu...

Ngày tải lên: 10/04/2014, 22:13

... involved in DNA repair, a 32 P-labeled DNA probe is prepared so that it contains the DNA lesion or DNA structure of inter- est. To mask the effects of nonspecific DNA binding proteins, the DNA probe is ... Non- checkpoint DNA repair mutants, when incubated with DNA- damaging agents die with a highly elongated phenotype, because they are unable to repair the DNA damage....

Ngày tải lên: 11/04/2014, 00:59

Ngày tải lên: 12/12/2013, 08:27

... transferase. Keywords: deoxyribose phosphate lyase; DNA polymerase; DNA repair enzyme; terminal deoxyribonucleotidyl trans- ferase. Ten years ago, only five DNA polymerase species (a to e) were known, ... elucidation of mechanisms of DNA repair in the cells in which DNA or does or does not replicate. We report here the characterization of Pol k from a higher plant, rice, using cD...

Ngày tải lên: 07/03/2014, 15:20

... Center for the Repair System of Damaged DNA, Korea Advanced Institute of Science and Technology, South Korea 2 Korea Basic Science Institute, Daejon, South Korea Nucleotide excision repair (NER) ... 2467–2476 ª 2005 FEBS Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B Byoungkook Kim 1, *, Kyoung-Seok Ryu 2, *, Hyun-Jin Kim 1 ,...

Ngày tải lên: 07/03/2014, 17:20

... various DNA repair mechanisms, the repair of a specific DNA damage is often conserved from bacteria to human, and in many cases the proteins are highly similar [5]. Little is known about DNA repair mechanisms ... gondii DNA repair enzyme; RRM, RNA recognition motif; HCA, hydrophobic cluster analysis; MMC, DNA- damaging agent mitomycin C; G-patch, glycine rich motif; SF45, spli...

Ngày tải lên: 08/03/2014, 22:20

... inactivate DNA repair processes by t he oxidation of zinc finger structures in DNA repair proteins. As these observations are derived from subcellular systems, experiments on interactions with DNA repair ... involved in DNA damage signaling and repair, such as poly(ADP-ribose) polymerase-1, formami- dopyrimidine -DNA glycosylase (Fpg), which is involved in the repair of cert...

Ngày tải lên: 30/03/2014, 15:20

... of DNA during replication, repair and transcrip- tion processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleaved strand [1]. During the process, DNA ... in carrying out DNA religation [3]. Consequently, a normally transient break in DNA could become a long-lived double- stranded break upon collision of Topo I DNA complex and DNA re...

Ngày tải lên: 31/03/2014, 21:21

... using DNase I to nick the DNA and the DNA polymerase I holoenzyme to label the DNA by nick translation gives less reproducible results than the fi ll-in method using Klenow fragment of DNA polymerase ... RNA. Wash the DNA pellet with 70% ethanol and resuspend in 100 µL deoxyribonuclease I (DNase I) digestion buffer. 3.2. DNA Hydrolysis 1. Follow steps 1–3, Subheading 3.1. 2....

Ngày tải lên: 10/04/2014, 22:13

... 5,6). 1.1. DNA Shape Circular plasmid DNA can exist in a number of different topological confor- mations. Superhelical circular DNA (form I), nicked circular DNA (form II), and linear DNA (form ... supercoiled DNA topoisomers migrate faster through agarose in comparison to linear DNA, nicked circular DNA, or relaxed DNA. For example, as shown in Fig. 1, nega- tively supercoile...

Ngày tải lên: 10/04/2014, 23:16