nutritional modulation of dna repair
Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
... radioprotective effect of the drug in this population Conventional analysis of the effects of DNA repair modifiers upon clonogenic survival is limited to quantifying the magnitude of change of a single ... comparison of the parameters in the treated and untreated experiments As a consequence, quantitative assessment of the effect of modifiers of DNA repair upon four distinct components of the radiation ... presence and absence of chemical inhibitors of PARP activity The variable nature and magnitude of the effects of PARP inhibition on clonogenic survival among these cell lines offered a useful setting...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... characteristics of the 275–284 segment of hHR23B Because of the high quality of our 15 N-HSQC spectra, 54 of the 58 protonated backbone nitrogen atoms were available for relaxation measurements The values of ... (25 instead of 27 °C), which gives better HSQC spectra Because of the known dependence of the overall correlation time sc on the molecular size of various proteins [23], both sc values of the XPCB ... The N-terminal part of XPCB–hHR23B B C Fig NMR structure of the XPCB domain of hHR23B (A) Stereoview of the 12 superimposed structures of XPCB–hHR23B All Pro residues conserved among the STI1...
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Báo cáo khoa học: End-damage-specific proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx
... next stage of repair Therefore, Pol b initiates repair of single-nucleotide gaps, PNK is required for the initiation of repair of 3¢-phosphatecontaining SSBs, and APE1 initiates repair of 3¢-phosphoglycolate-containing ... nicked DNA [18] Therefore, when repair enzymes are in excess over the amount of DNA SSBs, neither the cross-linking efficiency of XRCC1 DNA ligase IIIa heterodimer and Pol b nor the rate of repair ... gap-containing DNA Outline of cross-linking assay We have recently developed a new DNA protein cross-linking protocol aimed at revealing the engagement of BER proteins during repair of damaged DNA [15]...
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Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H. pylori DnaB helicase activity pptx
... presence of a different amount of HpSSB followed by separation of the DNA protein complex using agarose gel electrophoresis The retardation pattern of the ssDNA reveals the binding of HpSSB with ssDNA ... Effect of HpSSB on ATPase activity of HpDnaB in the presence of ssDNA The release of radiolabeled Pi from (c-32P)ATP was monitored in the absence and presence of different concentrations of HpSSB ... position of the ssDNA in the absence of SSB protein and the subsequent retardation of the band with an increasing amount of SSB is also shown *Position of the double-stranded control DNA (D) Localization...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... involved in DNA damage signaling and repair, such as poly(ADP-ribose) polymerase-1, formamidopyrimidine -DNA glycosylase (Fpg), which is involved in the repair of certain types of oxidative DNA base ... where N ¼ the number of strand breaks per molecule of PM2, I ¼ the percentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA The overall number of strand breaks per ... the event of diminished XPA DNA binding, a new band of even slower migration appeared (band 3), indicative of high molecular mass DNA protein complexes Similarly, complete inhibition of oligonucleotide...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
... (often choline) The nucleases catalyze the hydrolysis of DNA phosphodiester bonds Sequence alignments of PLDs revealed that, with the exception of two nucleases, most PLDs contain two copies of ... length) The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of mM of substrate by 0.125 lM of enzyme in reaction ... colorimetric assay in a 96-well format, the identification of a cofactor, and characterization of kinetic parameters of the human tyrosyl -DNA phosphodiesterase activity EXPERIMENTAL PROCEDURES Materials...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... component of total DNA breakage, and that the inhibition of apoptosis allows for more proper estimation of radiation-induced DNA DSB repair and apoptosis itself First, we found spontaneous cleavage of ... that the pattern of DNA smears consisted of a compression zone (CZ) just below the wells, which was more patent in irradiated cells, followed with an area of distribution of DNA fragments ending ... the fragility of agarose plugs, the corners tended to break off easily Therefore, to ensure that the same number of cells (DNA) per lane was loaded into a gel, we cut a section of the better...
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báo cáo khoa học: "Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening" doc
... 10 ug of total cell protein (C) Detection of MLH1 and MSH2 proteins on western blots with a mixture of varying amounts of HCT116 and LoVo cell lysates Results show that the combinations of these ... expression of these proteins (Figure 2C) Moreover, PHA treatment of isolated, fresh monocytes did not enhance MSH2 and MLH1 expression Analysis of fresh lymphocytes (PHA treated) from a cohort of patients ... F Density of the bands of interest on a western blot was determined by scanning of the x-ray film and highlighting the band area using a BioRad Gel 2000 documentation system and its software The...
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Báo cáo y học: "Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus" pot
... http://arthritis-research.com/content/13/2/R63 Page of Figure Decrease in the expressions of three DNA repair genes The expression levels of (a) excision repair cross-complementing (ERCC)2, (b) ERCC5, and (c) X-ray repair cross-complementing ... Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control of T cell ... pigmentosum complementation group G (XPG), also encodes a DNA repair protein ERCC5 is involved in excision repair of UV-induced DNA damage Mutations of this gene cause XPG syndrome or Cockayne syndrome...
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Báo cáo y học: " Tight glucose control: should we move from intensive insulin therapy alone to modulation of insulin and nutritional inputs" pps
... opinions of care givers, SPRINT may not reduce workload because it requires measurements every hour or hours Ultimately, evaluation of any TGC protocol must also include an assessment of its ability ... development [14] The monocentric study of Chase and coworkers [1] may not ensure the ‘exportability’ of their TGC protocol The debate continues about the real benefits of TGC, with numerous questions ... results from instructions regarding nutritional intake, allowing insulin infusion rates to be limited to a level lower than usual, or from the intrinsic quality of the algorithm used, which is based...
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Báo cáo y học: "Location of DNA damage by charge exchanging repair enzymes: effects of cooperativity on location time" doc
... the stretch of DNA ahead of it is thus error-free Presumably the electron received destabilizes the binding of MutY to this error-free stretch of DNA by changing the oxidation level of the [4Fe-4S]3+ ... 8-oxo-G DNA glycosylase hOGGl and the E coli DNA glycosylase AlkA kink error-free DNA in the same way they kink DNA during the excision of a damaged base [15] It is thus likely that some DNA glycosylases ... excised out of DNA and into a cleft in the DNA glycosylase This extra-helical state is associated with a kinking of the DNA through an angle of 60°–80° depending on the particular DNA glycosylase...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
... Woods The Influence of the Ku80 Carboxy-Terminus on Activation of the DNA- Dependent Protein Kinase and DNA Repair is Dependent on the Structure of DNA Cofactors In mammalian cells DNA double strand ... phosphorylation target of DNA- PK55 Due to its ability to bind ssDNA, RPA has been shown to be involved in several nuclear pathways including DNA replication and DNA repair2 In terms of the repair of double ... and sequence of DNA can modulate DNA- PK activity This is of great interest because DNA- PK must respond to a large variety of DNA structures and sequences in order to efficiently repair DSBs How...
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FUNCTIONAL ANALYSIS OF TWO NOVEL DNA REPAIR FACTORS, METNASE AND PSO4
... auto-phosphorylation, DNA- PKcs mediates recruitment of the XRCC4 -DNA Ligase IV complex to the DBS site (57-59) The recruitment of the XRCC4 -DNA ligase IV complex is essential for the final step of ligation ... The promotion of DSB repair by the SET domain of Metnase suggests that Metnase may function in opening chromatin, as well as enhancing accessibility of repair factors In support of this hypothesis, ... promoted integration of retroviral DNA (13, 14) Metnase promotion of the integration of widely varied exogenous DNA sequences integration indicates that this activity is DNA sequence-independent...
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ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU80 C-TERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU
... Purity of Ku80CTR 26 DNA binding activity is not affected by truncation or the addition of Ku80CTR 27 The effects of oxidation on DNA binding of wtKu and Ku70/80C 29 Effects of ... for efficient activation of DNA- PKcs, and only the final 12 amino acids are sufficient to bind DNA- PK (31-33) Upon removal of the C-terminus of Ku80 the kinase activity of DNA- PK is drastically ... C-terminus of Ku80 as an effecter of DNA- PKcs activation and potentially involved in redox regulation of Ku DNA binding activity These analyses showed that the removal of the extreme C-terminus of Ku80...
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INHIBITION OF APE1’S DNA REPAIR ACTIVITY AS A TARGET IN CANCER: IDENTIFICATION OF NOVEL SMALL MOLECULES THAT HAVE TRANSLATIONAL POTENTIAL FOR MOLECULARLY TARGETED CANCER THERAPY
... deficiency of a particular DNA repair pathway in cancer cells can lead to elevated levels of other DNA repair pathway proteins leading to efficient repair of DNA damage and reducing the efficacy of cancer ... persistence of unrepaired DNA damage results in cell cycle arrest, apoptosis and accumulation of mutations (61, 127) To protect cellular DNA, several DNA repair pathways such as the Base Excision Repair ... that since Ape1 is involved in the repair of DNA damaged by chemotherapeutic agents, identification of a small molecule inhibitor of the DNA repair activity of Ape1 protein using a high-throughput...
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Role of BRCA2 in DNA repair
... 3.6 Exon11 BRCA2 renders transfected cells more sensitive to specific DNA lesions 56 3.6.1. Analysis of DNA double strand break repair 56 3.6.2. Analysis of DNA repair capabilities 60 3.7 E11 and CT region of BRCA2 support AGT interaction ... Figure 1, Mode of action of AGT in DNA repair .14 Figure 2, Mode of killing by methylating and chloroethylating drugs 17 Figure 3, Diagram depicting the main functional domains of BRCA2 ... Figure 4, BRCA2 recruits Rad51 to sites of DNA damage and promotes nucleation of the Rad51 filament to sites of DNA double strand breaks 21 Figure 5, BAC DNA integrity is intact after successful 105 bp deletion...
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Tài liệu Growth and nutritional status of children with homozygous sickle cell disease ppt
... has been limited evaluation of a variety of nutritional interventions that could influence the natural history of SCD.161 Improving the nutritional status and growth of these children could have ... levels of LH and FSH were higher in patients than in controls at the same stage of development of secondary sexual characteristics This suggested a variation in the rate of maturation of the ... were admitted more often.11 In pre-pubertal Jamaican children, levels of haemoglobin (Hb) and fetal haemoglobin (Hb F) decreased with an increasing number of hospitalisations of both sexes, although...
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Tài liệu Nutritional Care of the Housebound Elderly pptx
... of who they are We hope the meeting of November 2005 will increase awareness of the community, of governments, NGOs and professionals so that Nutritional Care of the Housebound Elderly will receive ... copies of this set of notes to all who attended and hope to have it on a Website soon as well Stewart Truswell, AO, MD, DSc, FRACP Nutritional Care of the Housebound Elderly NUTRITIONAL CARE OF HOUSEBOUND ... Associate Professor Susan Quine, School of Public Health, University of Sydney Dr Michael Fine, Senior Lecturer, Department of Sociology, Macquarie University Sheila Rimmer, AM, Chair of NSW Home...
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Tài liệu Nutrition and Nutritional Care of Elderly People in Finnish Nursing Homes and Hospitals pptx
... overestimate of the true situation of how effective nurses pay attention to nutritional problems among their patients Nutrition education of professionals The nutrition education of professionals ... years and older only 11.3% of males and 14.5% of females were obese (Health, 2000) 2.7 NUTRITIONAL ASSESSMENT OF ELDERLY PEOPLE The full assessment of elderly people’s nutritional status includes ... malnutrition is often unrecognized and there is a lack of documentation on the nutritional deficiencies in nursing homes (Abbasi and Rudman, 1993) A knowledge of nutritional status of aged people...
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Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... solution of ThT and the fluorescence intensity of the solution was measured at 482 nm Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 ... formation of aggregates of the protein on exposure to dopamine [16] In vitro experiments probably provide a better understanding of the role of various interacting components The formation of dopamine–quinone ... measurement of ThT fluorescence would be an important probe for characterization of the nature of the aggregates Characteristic sigmoidal curves of amyloid-type aggregates, Fig Aggregation of a-synuclein...
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