dna repair molecules and cancer therapeutical responses

... DNA repair; however, deficiency of a particular DNA repair pathway in cancer cells can lead to elevated levels of other DNA repair pathway proteins leading to efficient repair of DNA damage and ... inhibit DNA repair pathway proteins since cancer promotion and deregulated cellular growth is aided by deficient DNA repair pathways, a fine balance exists between induction of DNA damage and its ... contradictory to inhibit DNA repair pathway proteins since cancer promotion and deregulated cellular growth is aided by deficient DNA repair pathways, it actually makes sense to block DNA repair given the...

Ngày tải lên: 24/08/2014, 13:10

... (Thr241Met) polymorphism and lung cancer risk Cancer Epidemiol Biomarkers Prev 2001; 10: 911-2 26 Chen S, Tang D, Xue K, et al DNA repair gene XRCC1 and XPD polymorphisms and risk of lung cancer in a Chinese ... the DNA repair gene XPD and risk of primary lung cancer Lung Cancer 2002; 36: 15-6 28 Harms C, Salama SA, Sierra-Torres CH, et al Polymorphisms in DNA repair genes, chromosome aberrations, and ... studies of common polymorphisms in DNA repair genes, if large and unbiased, can provide insight into the in vivo relationships between DNA repair genes and lung cancer risk Such studies may identify...

Ngày tải lên: 31/10/2012, 14:59

... substrate of ATM and ATR during DNA damage and DNA replication checkpoint responses J Biol Chem 279:53353–53364 You Z, Kong L, Newport J (2002) The role of single-stranded DNA and polymerase alpha ... type of DNA damage is coupled to a specific DNA repair pathway as well as the cell cycle regulatory apparatus Certain types of Checkpoint and Coordinated Cellular Responses to DNA Damage 73 DNA damage ... ATR after DNA dam- Checkpoint and Coordinated Cellular Responses to DNA Damage 75 age interfering with DNA replication (Brown and Baltimore 2003; Cortez 2003) The phosphorylation of Chk1 and Chk2...

Ngày tải lên: 25/10/2013, 21:20

... BER but also in DNA doublestrand break repair and meiosis, and Pol b and TdT may be evolved for differentiation and development of specific organs in vertebrates such as the nervous and immune systems ... meristem and panicle (lanes and in Fig 5A), and showed Plant DNA polymerase k (Eur J Biochem 271) 2805 moderate expression in the roots, root apices, flag leaves and young leaves (lanes 3, 4, and 7) ... involved in DNA repair and cell cycle checkpoint regulation on DNA damage [13] shows a lower degree of homology to human Pol k To determine the phylogenetic relationship between OsPol k and other...

Ngày tải lên: 07/03/2014, 15:20

... with orientations that differ between hHR23B and hHR23A are shown Also, b-N and b-C are the N- and C-termini of XPCB–hHR23B; a-N and a-C are the N- and C-termini of hHR23A FEBS Journal 272 (2005) ... (HT1 and HT2) were identified in XPCB–hHR23B, one between helix and helix 3, and one between helix and helix 4–5 These two trails were linked by a hydrophobic linker patch between helix and helix ... related to XPC and DNA repair [19] Here, we report the three-dimensional solution structure of the XPCB–hHR23B (275–342) fragment (XPCB–hHR23B), which contains the XPC interaction domain, and compare...

Ngày tải lên: 07/03/2014, 17:20

... involved in DNA repair and RNA metabolism in the protozoan parasites such as T gondii and the Plasmodia Further analyses should contribute to our understanding of the biochemistry of DNA repair proteins ... isolation and sequencing of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position of the two introns which were 780- and ... P.C (1999) A phylogenomic study of DNA repair genes, proteins, and processes Mut Res 435, 171– 213 Marti, T.M., Kunz, C & Fleck, O (2002) DNA mismatch repair and mutation avoidance pathways J...

Ngày tải lên: 08/03/2014, 22:20

... helicase and is required for the repair of damaged DNA Thus, in agreement with a role of Tip60 in DNA repair, the expression of a mutant version of Tip60 causes defects in DNA repair and reduces ... J Biochem 271) 2345 deposits histones onto DNA that was subject to DNA repair synthesis, and it is recruited to sites of NER and singlestrand break repair, probably through its interaction with ... play during DNA replication and the passage of DNA polymerases and helicases In light of this, it is interesting to note that the FACT components Spt16 and Pob3 are found associated with DNA polymerase...

Ngày tải lên: 16/03/2014, 16:20

... Damage, Repair and Misrepair in Cancer and in Cancer Therapy 177 Ana M Urbano, Leonardo M R Ferreira, Joana F Cerveira, Carlos F Rodrigues and Maria C Alpoim Chapter DNA Repair, Cancer and Cancer ... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA...

Ngày tải lên: 27/06/2014, 19:20

... Damage, Repair and Misrepair in Cancer and in Cancer Therapy 177 Ana M Urbano, Leonardo M R Ferreira, Joana F Cerveira, Carlos F Rodrigues and Maria C Alpoim Chapter DNA Repair, Cancer and Cancer ... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA...

Ngày tải lên: 28/06/2014, 03:20

... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... locally unwound DNA (Bell and Dutta, 2002; Dutta and Bell, 1997) and promoting the assembly of the multienzyme complexes required 26 DNA Repair − On the Pathways to Fixing DNA Damage and Errors for ... eukaryotic single-stranded (ss) DNA binding protein and it is required for DNA replication, recombination and repair RPA helps recruit DNA primase/polymerase α to the origins, stabilizing ssDNA in the...

Ngày tải lên: 28/06/2014, 05:20

... means that DNA breakage and repair occurs in a non-physiological environment Whitaker and McMillan reported in 1992 that encapsulating cells before irradiation impair the estimation of DNA repair ... estimate DNA repair Nevertheless, the underlying mechanisms affecting cells embedded in agarose during DNA repair in PFGE methodology are still not well understood To better understand spontaneous DNA ... DNA double-strand break rejoining in human tumour cells Br J Cancer 1995, 71:311-316 Whitaker SJ, McMillan TJ: Pulsed-field gel electrophoresis in the measurement of DNA double-strand break repair...

Ngày tải lên: 09/08/2014, 09:20

... Donadi E: Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control ... genetic susceptibility in SLE [20] Bassi and colleagues, however, reported that SLE leucocytes less efficiently repair radiation-induced DNA damage and that DNA repair gene polymorphic sites may predispose ... to mitochondrial dysfunction Also worth noting is that repairs of UV-induced DNA damage by ERCC2/XPD and ERCC5/XPG requires ATP The ATP and DNA binding regions are contained in ERCC2/XPD [24] Most...

Ngày tải lên: 12/08/2014, 15:23

... three DNA immunizations delivered by tattoo (p < 0.0001) Both GM-CSF DNA co-delivery and cardiotoxin pre-treatment enhanced the L1-specific humoral responses after both three and four HPV16-L1 DNA ... GM-CSF and cardiotoxin substantially enhanced the immune responses after intramuscular DNA immunization The advantage of tattoo treatment is the low price of the tattoo device and a standardized ... antigen-specific responses [6,8] Compared to intra-muscular injection of DNA, delivery of DNA by tattooing seems to produce different gene expression patterns In one study, tattooing of 20 μg DNA resulted...

Ngày tải lên: 14/08/2014, 19:22

... kinase-like protein kinases FAT FRAP, ATM, and TRRAP C-terminus Carboxy terminus dsDNA double-strand DNA ssDNA single-strand DNA SAP SAF-A/B, Acinus, and PIAS domain VHS Vps27p/Hrs/STAM XLF XRCC4-like ... downstream molecules DNA termini are processed and DNA- PK dissociates from the DNA DNA Ligase IV/XRCC4-XLF complex facilitates ligation Adapted from reference17 1.3 DNA Damaging Agents in Cancer ... to bind 50fmol of DNA Titration experiments were performed and demonstrate that these concentrations of Ku and DNA were in excess and DNA- PKcs is the limiting factor 2.8 DNA- PK DNA Binding/Recruitment...

Ngày tải lên: 24/08/2014, 09:33

... sequence-independent DNA binding activity, favoring double-stranded DNA over single-stranded DNA I demonstrated that the two proteins form a 1:1 stochiometric complex, and once formed, Metnase can localize to DNA ... cells, DNA double-strand breaks induced by IR and V(D)J recombination are mainly repaired by nonhomologous end-joining (NHEJ) (1-5) The NHEJ repair proteins Ku70/80, DNA PKcs, Artemis, and Xrcc4/ligase ... urea) for DNA cleavage DNA size makers indicated on the left B) Kinetic analysis of Metnase’s DNA cleavage activity with partial duplex DNA (3’- and 5’-overhangs) M1 and M2 represent two DNA markers...

Ngày tải lên: 24/08/2014, 10:50

... (NHEJ) DNA Repair Pathway A DSB occurs and the pathway is initiated by the Ku protein binding damaged DNA termini, translocates inward, and recruits DNA- PKcs to form the DNA- PK heterotrimer DNA- PK ... instability and contribute to the development and progression of cancer An increasing amount of research is drawing a close correlation between DNA repair and how it affects the development and treatment ... OF REDOX EFFECTS AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can occur as...

Ngày tải lên: 24/08/2014, 11:02

... frequent types of cancer diagnosed in the United States in decreasing incidence are breast cancer, prostate cancer, lung cancer and colon cancer; yet lung cancer is the deadliest form of cancer in the ... Cdc28 and classes of vertebrate cyclins (D- and E-type cyclins in G1 and A -and B-type cyclins in G2/M) that control a family of Cdks (cyclindependent kinases) greatly enhanced our understanding ... 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested...

Ngày tải lên: 25/10/2013, 21:20

... cancers, gastrointestinal and other associated cancers, lung and upper aerodigestive cancers and prostate and urologic cancers, each have biomarkers in prevalidation and validation phases in which ... methylated DNA sequences and other biomarkers have been identified as promising biomarkers for bladder and prostate cancers; and mutations and deletions in mitochondrial DNA were detected in prostate and ... Texas M D Anderson Cancer Center Colorectal and Other Gastrointestinal Cancers 35 Colorectal Cancer Colon cancer is the third most frequently diagnosed cancer in the United States and the third...

Ngày tải lên: 14/02/2014, 21:20

... skin, and lungs are discarded every few days; deoxyribonucleic acid (DNA) repair enzymes, which repair DNA that was damaged from many different sources; and detoxication enzymes of the liver and ... bioassays and are carcinogenic (Gold et al., 1997b) Dehydroepiandrosterone (DHEA) and DHEA sulfate are the major secretion products of adrenal glands in humans and are precursors of androgenic and ... Stofberg and Grundschober (1987) 800 Stofberg and Grundschober (1987), Lorenz and Maga (1972) Stofberg and Grundschober (1987), Arkima (1968) (7300) TAS (1989), Nelson and Hoff (1969), Kazeniac and...

Ngày tải lên: 14/02/2014, 22:20

... with cancer of the mouth, larynx, pharynx and esophagus, and heavy beer drinking is associated with cancer of the rectum Cancer can develop anywhere along the gastrointestinal tract, but colon cancer ... Nutrition and Health, stomach cancer has been found among populations who eat lots of pickled vegetables (Japan), salted fish (Norway) and smoked trout and mutton (Ireland) Esophageal cancer is ... certain types of cancer, especially if you drink and smoke Choose high-fiber foods Besides fresh fruits and vegetables, add more whole grain breads and cereals and legumes (dried peas and beans) to...

Ngày tải lên: 15/02/2014, 04:20