embryonic stem cells, methods and protocols - kursad turksen
embryonic stem cells, methods and protocols - kursad turksen
... Embryonic Stem Cells: Methods and Protocols Edited by: K. Turksen © Humana Press Inc., Totowa, NJ 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L. Roach and John ... until used and discard after 14 d. Murine Embryonic Stem Cells 3 HUMANA PRESS HUMANA PRESS Methods in Molecular Biology TM Methods in Molecular Biology TM Embr...
Ngày tải lên: 08/04/2014, 12:51
neural stem cells. methods and protocols
... Liposome Methods and Protocols, edited by Subhash C. Basu and Manju Basu, 2002 198. Neural Stem Cells: Methods and Protocols, edited by Tanja Zigova, Paul R. Sanberg, and Juan R. Sanchez-Ramos, ... Cytogenetics: Methods and Protocols, edited by Yao-Shan Fan, 2002 203. In Situ Detection of DNA Damage: Methods and Protocols, edited by Vladimir V. Didenko, 2002 202. Th...
Ngày tải lên: 11/04/2014, 09:58
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... 5¢-TGCGCTCTATTTCCTCCTTG-3¢; mouse GAPDH,US5¢-GATGATGGAGGACGTGATGG-3¢ and DS 5¢-GGCTGCAGGAGAAGAAAATG-3¢; mouse Nec- din,US5¢-TTTACATAAGCCTAGTGGTACCCTTCC-3¢ and DS 5¢-ATCGCTGTCCTGCATCTCACAGTCG-3¢. Antibodies TBP sc-273, ... 5¢-GCTGTGACATGGA AACTATG-3¢ and DS, 5¢-GGACTTTCTCAGTATGA CATG-3¢; humanHS3RT: US, 5¢-CCA CCAG CTATCA GGGCCCAG- 3¢ and DS, 5¢-GCTGCTATGCTGTGCCTC- 3¢; human5¢HS2: US,...
Ngày tải lên: 07/03/2014, 12:20
Glycoprotein Methods and Protocols - P1
... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and subjected ... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based meth- ods such as slot-blotting and stainin...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... mounting, and its relative stability. There are, however, several other alternatives, such as 3-amino- 9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro- 3-indolyl ... side chain substituted O-acetylated sialic acids: the PAT- KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures. Histochem. J. 16, 623–639. 8. Veh, R. W., Meesen, D., Kuntz, D., and May, B. (198...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido- 2-deoxy- - D -galactopyranoside, without adversely affecting cells (9). With the eluci- dation of more sequence data ... carbohydrate or core pro- tein specificity), and lower sensitivity than slot-blot and immunoassay methods. Chap- Detection and Quantitation of Mucins 47 ter 1 (2) discusses these assays and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... mL of glycerol. 8. Poly- L -lysine-coated slides: slides are immersed in 0.01% (w/v) poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous ... rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195. 4. Bell, A. E., Allen, A., Morris, E. R., and Ross-...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... interest in the homogenate and medium by Western-type dot-blot procedure. Spot aliquots of the homogenates or media on nitrocel- lulose paper, using the dot-blot apparatus and air-dry for 5 min. Perform ... [ 35 S]sulfate-labeled, TCA-precipitable proteins in tis- sue homogenate (j1) and medium (j2) (cpm/mL) (Note 10). k. Mature [ 35 S]sulfate-labeled mucin band in tissue homogenate (k1...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... prepared from the gel-forming mucins isolated from other epithelial secretions (respiratory and cer- vical) and from intestinal cell lines (HT-29 and PC/AA). In this example the electro- phoretic mobility ... monitored with a periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the relative merits and dr...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... Swing-out rotors and tubes (see Note 1). 5. Gradient maker (see Note 2). 8 96 Sheehan and Thornton 12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara- tion ... M...
Ngày tải lên: 08/10/2012, 10:25