embryonic stem cells and cardiac progenitors sources of cardiomyocytes

... pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent cells ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... expressed in epiblast stem cells (EpiSCs) and primordial germ cells (PGCs) 12 The importance of this transcription factor is evidenced by the fact that the ablation of Oct4 results in embryonic lethality...

Ngày tải lên: 09/09/2015, 18:56

... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of...

Ngày tải lên: 11/08/2014, 12:21

... and genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells ... transposition system, in which a transiently expressed transposase catalyzes the excision of transgenes flanked by inverted terminal repeats [8,9] The PB system is effective in mouse and human cells, and ... prolonged period of time (usually weeks for mouse cells, and 3-4 weeks for human cells) Whether reprogramming by defined factors can be further optimized will depend on a better understanding of the mechanisms...

Ngày tải lên: 14/08/2014, 21:20

... Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on MEF feeders 38 4.2.2 Feeder- free Culture of Human Embryonic Stem Cells 39 4.2.3 Coating of Matrigel ™ ... development of cardiac cells from IPSCs 14 Literature Review 3.1 Human Embryonic Stem Cells (hESCs) Human Embryonic Stem Cells are pluripotent cells derived from the inner cell mass of the developing ... Aims and objectives 14 Literature Review 15 3.1 Human Embryonic Stem Cells (hESCs) 15 3.2 Porcine Embryonic Stem Cells 16 3.3 Generation of Induced Pluripotent Stem Cells...

Ngày tải lên: 30/09/2015, 10:13

... experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass ... pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the...

Ngày tải lên: 22/03/2014, 09:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the functioning, and physioand ... regeneration and repair [43] Systemic injection provides a model of choice for delivering adult derived-neural progenitor and stem cells for the treatment of neurological diseases and injuries,...

Ngày tải lên: 08/08/2014, 17:20

... m l of α-synuclein protein in the form of Lewy bodies and Lewy neurites, and selective loss of dopamin­ rgic (DA) e neurons in the substantia nigra of the mid­ rain [68,69] b The majority of PD ... study of live cell and early aspects of disease pathogenesis For AD, there are many attractive targets for this type of analysis, including the toxicity, clearance and localization of Aβ and other ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F,...

Ngày tải lên: 11/08/2014, 12:21

... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture...

Ngày tải lên: 09/08/2014, 14:22

... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... human embryonic stem cells Cell 2005, 122:947-956 Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA: Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells ... References Young RA: Control of the embryonic stem cell state Cell 2011, 144:940-954 Ben-David U, Benvenisty N: The tumorigenicity of human embryonic and induced pluripotent stem cells Nat Rev Cancer...

Ngày tải lên: 11/08/2014, 12:21

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout ... Materials and methods Isolation and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1...

Ngày tải lên: 12/08/2014, 11:23

... M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and ... the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the H9 line isolated by Thomson and colleagues...

Ngày tải lên: 31/10/2012, 16:57

... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing...

Ngày tải lên: 07/03/2014, 12:20

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational...

Ngày tải lên: 18/06/2014, 15:20

... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... like to thank Professor Huang Lin and Miss Winnie lee from the Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, for their assistance in cells culture and related evaluation...

Ngày tải lên: 20/06/2014, 00:20

... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

Ngày tải lên: 20/06/2014, 04:20

... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... least doubled the number of CFU-Fs present while enhancing the expression of oct-4 and rex-1, genes expressed by embryonic stem cells and thought to be pivotal in maintaining ‘stemness’ These data ... is the homing of stem cells to sites of injury and subsequent wound healing Although some tissue repair may be accomplished by the division of indigenous differentiated cells, such cells are most...

Ngày tải lên: 09/08/2014, 10:20

... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... final concentration of 300 nmol/l Lipofectamine 2000 at 2% (vol/ vol) and chordin-siRNA were used in a total transfection volume of 511 μl/well Appropriate cell, Lipofectamine 2000 and negative siRNA ... aged 71, 76 and 78 years, and a male aged 77 years Briefly, the marrow cells were layered on Ficoll (Sigma-Aldrich) and centrifuged for 30 minutes at 400 g The mononuclear cells Page of (page number...

Ngày tải lên: 09/08/2014, 10:23

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...

Ngày tải lên: 10/08/2014, 05:21