MECHANICAL AND METALLURGICAL ANALYSIS OF STRUCTURAL STEEL pot

... design, service, and abnormal loads, and aircraft impact damage on the structural, fire protection, and egress systems. Mechanical and Metallurgical Analysis of Structural Steel; Project Leader: ... Gayle Determine and analyze the mechanical and metallurgical properties and quality of steel, weldments, and connections from steel recovered from WTC 1, 2, and 7. Investigation of Active Fire ... evaluating the structural performance of the buildings and behavior and fate of occupants and responders. Structural Fire Response and Collapse Analysis; Project Leaders: Dr. John L. Gross and Dr....

Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx

... residues of D3 and B contribute to thestability of t he dimer. The ﬁrst c luster includes F70/V336 and I72/Q338 ( both in b5 s trand) of D3 and F 27/Y289(b2 strand), L67/M324, V70/I327 and L72/L328 ... b5 strand) of D3 and I41/L304,C43/A306 ( both in b3), L69/S326 and L71/L328 (both inb4) of B. Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 of ... b4strand) of B. The second cluster c onsists of P6/M267, L10/L271 (both i n a-helix), V18/C279 (b1 s trand), L32/F293 (b2strand), I33/K294 (loop after b2 strand), I68/F334, L71/V337 and L73/F339...

Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot

... expanded. FACS analysis was also performedto ensure uniform expression of GFP in the selected celllines, and anti-14-3-3f immunoblotting analysis of lysedcells was performed to ensure a band ... signal trans-ducer and activator of transcription 3 (STAT3), and hematopoietic progenitor kinase 1-interacting protein of 55 kDa (HIP-55). Using both MS and MALDI-TOF ⁄ TOF MS tryptic mass ﬁngerprinting, ... percentage of breasttumors in the early stage of the disease, contributingto the transformation of cells and also to the furtherprogression of breast cancer [42]. On the other hand,downregulation of...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... thepresence of Pex1p and Pex6p by SDS ⁄ PAGE and immuno-blot analysis with antibodies against Pex1p, Pex6p and anti-(rabbit IgG).MicroscopyPotassium permanganate ﬁxation and preparation of intactyeast ... Here we conﬁrm and extend earlier studies of these AAA-peroxins and givea further detailed functional analysis of their cassettestructure and interaction.The interaction of Pex1p and Pex6p involvestheir ... biologicalfunction of Pex1p and Pex6p.ABFig. 2. Two-hybrid interaction of Pex1p and Pex6p harboring pointmutations of the WalkerA and B motifs of their ATP-binding sites.A schematic representation of the...

Equipment for older and disabled people: an analysis of the market potx

... transfers into and out of beds and chairs, and specialist mattresses and pressure-relieving seating, can reduce the likelihood of pressure sores and ulcers (which is a major cost to the NHS), and unnecessary ... million, and that of stairlifts is in the region of £150 million. Because of the sheer range of products that can be described as ‘community equipment and the lack of publicly available and reliable ... ortheses and cosmeses come in ‘all shapes and sizes’ and cover a vast range of products and devices, from heart valve replacements to shoe insoles and a variety of artificial limbs and directly...

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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot

... lm) and programmed at an initial temperature of 45 °C for 1 min, with a ramp of 10 °CÆmin)1to 280 °C, and ﬁnal time of 10 min. Products were identiﬁed by compar-ison of retention times and ... formation of identical products.The characterization of product speciﬁcity by functionalexpression in E. coli of the monoterpene synthases of lemon conﬁrmed that both C62 and M34 (Cl(+)LIMS1 and ... epi-aristolochene and trichodiene synthase containthree Mg2+ions in their active site, two of which arechelated by the DDXXD motif of the active site and a thirdwhich is liganded by a triad of active...

Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

... Secondary and tertiary structure of TenA. (A) Amino acid sequence of HP-TenA. The beginning and end of secondary structureelements of HP-TenA are shown in the bottom line. (B) Stereo view of a cartoon ... reminis-cent of that of human heme oxygenase-1 [13].In the present study, we present the crystal structure of the HP1287 gene product as well as one of itsmutants (F47Y) and discuss the in vivo role of H. ... part of the cavity, which is connected to thesolvent through a long tunnel, is lined by residuesPhe210 and 47, Trp211, Tyr51 and 139, Asp44 and Glu207 (Fig. 3A). In one of the two monomers of...

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... (K20.667 [30]). A value of 1.36 was taken for therefractive index n [31].MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after trypticdigestion of the labeled and nonlabeled Ag-NPA-1 ... goodcorrespondence of the experimentally determined pI of 6.1 and the theoretically deduced pI value of 6.22 wasfound.A theoretical prediction of the secondary structural organization of Ag-NPA-1 ... incorporation of the ligand. Binding of DAUDA to Ag-NPA-1 caused  20% quenching of the emission of Trp17 indicating FRET between thesingle Trp residue and the dansyl group. We calculateda value of 3.74...

Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

... exons and 11 introns, and anopen reading frame (ORF) of 1599 bp encoding a poly-peptide of 533 amino acids, with a predicted molecular mass of  57 kDa and a pI of 8.34.BLASTX analysis showed thatthe ... identiﬁcation of a hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNAfrom the grass carp. The gene spans  11 kb of genomicsequence and consists of 12 exons and ... Winnie W. L. Poon, Shuk H. Cheng and Richard Y. C. KongDepartment of Biology and Chemistry and Centre for Coastal Pollution and Conservation, City University of Hong Kong,Kowloon Tong, Hong...

Báo cáo khoa học: "A Linguistic and Computational Analysis of the German "Third Construction"*" potx

... consists of a finite-state control and of a stack of stacks. There are two types of moves: either an input is read and pushed onto a new stack on top of the stack of stacks, or a fixed num- ber of ... range of data. 2 A Linguistic TAG Analysis Following [den Besten and Rutten 1989], [Santorini and Kroch 1990] argue that the third construction, rather than being a morphological effect of ... A Linguistic and Computational Analysis of the German "Third Construction"* Owen Rambow Department of CIS, University of Pennsylvania Philadelphia, PA 19104,...

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