Báo cáo khoa học: Functional dissection of two Arabidopsis PsbO proteins PsbO1 and PsbO2 doc

... similar to PsbO1 . Ppu MI Bam HI PsbO1 PsbO2 PsbO1 -1-2 PsbO2 -1-1 PsbO1 -2-1 A B C Fig. 5. Oxygen-evolving activity of chimeric proteins derived from PsbO1 and PsbO2 . (A) A model of the chimeric PsbOs. ... 406–425. R. Murakami et al. Functional dissection of PsbO1 and PsbO2 FEBS Journal 272 (2005) 2165–2175 ª 2005 FEBS 2175 Functional dissection of...

Ngày tải lên: 30/03/2014, 16:20

... (B) Regulation of the forward and reverse Pta reactions. Pta catalyzes both the synthesis and degradation of acetyl-CoA. These two reactions are differentially regulated by pyruvate and PEP, which ... for Pta-F2 and Pta-F1. (B) Activation of the acetyl phosphate synthesis activity of E. coli Pta by different concentrations of pyruvate and PEP. Results are presented as pe...

Ngày tải lên: 06/03/2014, 11:20

... crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure of the ... structure of Ydc2 solved recently revealed the molecular basis of junction cleavage and threw some light on the mechanism of binding and manipulation of the junction [2...

Ngày tải lên: 20/02/2014, 11:20

... in two different nonoverlapping cosmids. Functional ana- lysis of dmdR1 and dmdR2 was performed by deletion of each copy. Dele- tion of dmdR1 resulted in the derepression of at least eight proteins ... analyzed by using the mascot soft- ware [29]. Immunodetection analysis of DmdR1 and DmdR2 Western blot analysis of DmdR1 and DmdR2, after SDS ⁄ PAGE resolution of the...

Ngày tải lên: 16/03/2014, 18:20

... pur- ification, and potential use of His-tagged G AL4 fusion proteins. Methods Mol. Biol. 63, 129–137. 17. Bradford, M.M. ( 1976) A rapid and sensitive me thod for the quantitation of microgram quantities of ... production and purification For the expression in and purification of recombinant proteins from Escherichia coli the QIAexpressionist TM system from Qiagen was used....

Ngày tải lên: 23/03/2014, 13:20

... absence of ligand, higher indu- cible levels of reporter activity in the presence of ligand, and high ligand sensitivity. We analyzed the DNA bind- ing, ligand binding, heterodimerization and transactiva- tion ... difference in ligand induced conformational changes as well as displacement of corepressors and recruitment of coactivators by EcR fusion proteins upon addition o...

Ngày tải lên: 16/03/2014, 12:20

... i.e. DIMs and PGL-tb; and (b) to address the question of the function of the two related molecules DIM A and DIM B. According to the accepted model of DIM and PGL biosynthesis [17], DIM A and PGL-tb ... FEBS 1969 Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and p...

Ngày tải lên: 23/03/2014, 09:20

... paper we pre- sent a detailed study of the structure and expression of the mce-1 gene in C. elegans. Results and Discussion Identification and sequence analysis of C. elegans MCE Searches in the ... elegans is folded into two tandem babbb modules each spanning around 60 amino acid residues. Within the two modules, the con- nectivity of the strands are b 1, b 4, b 3, b 2 and...

Ngày tải lên: 23/03/2014, 13:20

... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], and usually ... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II)...

Ngày tải lên: 18/02/2014, 06:20

... four molecules of 3OH-C12:0, two of C10:0, and one of C12:0 [10]. When compared to this struc- ture, the lipid A of O35ElpxX lacks two decanoic acyl (C10:0) substituents, and that of O35ElpxL lacks ... of the parental LOS. The LOS band of revertant O35ElpxX also showed a change from brown to black coloration (Fig. 4, lanes 3 and 5). Composition and MALDI-TOF MS analy...

Ngày tải lên: 18/02/2014, 14:20