Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx

... analysis of isoaspartyl peptidase activity of (a) EcAIII and (b) LlA. 3226 D. Borek et al. (Eur. J. Biochem. 271) Ó FEBS 2004 Expression, purification and catalytic activity of Lupinus luteus asparagine ... the expression, purification, and biochemical characterization of two homologous enzymes, with amido- hydrolase activities, of plant (Lupinus luteus pot...

Ngày tải lên: 30/03/2014, 15:20

... Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein Alinda Nagy, Ma ´ ria Trexler and La ´ szlo ´ Patthy Institute of ... in ovary, testis and brain, the most significant expression of the WFIKKN gene is observed in adult pancreas, liver and thymus. In view of the presence of WAP-, Kazal-, Kunitz-...

Ngày tải lên: 31/03/2014, 01:20

... complete distribution of consensus secondary structure estimated from the model is as follows: 39% of a-helices, 9% of b-sheets, 12% of turns, 21% of random coil and 19% of other structures. These estimations ... mammalian HDC catalytic site and those of the other group II L -amino acid decarboxylases: (a) HDC is the least efficient enzyme of its group; (b) mammalian HD...

Ngày tải lên: 08/03/2014, 08:20

... 5), and the influence of Cu 2+ ions (Table 3). Maximum activity was found at pH 3 and between 40 and 45 °C. Copper at 0.01 m M greatly stimu- lated the activity. The presence of Fe 2+ ,Mn 2+ and ... production of p-nitrophenol was measured spectrophotometrically at 405 nm. One unit of the acid phosphatase activity was defined as the amount of protein which catalysed the f...

Ngày tải lên: 07/03/2014, 14:20

... Coexpression, purification and characterization of the E and S subunits of coenzyme B 12 and B 6 dependent Clostridium sticklandii D -ornithine aminomutase in Escherichia coli Hao-Ping ... Clostridium sticklandii comprises two strongly associating subunits, OraS and OraE, with molecular masses of 12 800 and 82 900 Da. Previous studies have shown that in Escherichia col...

Ngày tải lên: 30/03/2014, 15:20

... BF2 and BF3 – were 15, 17 and 19 bp, respectively (Fig. 7A). The single-stran- ded region of the BF structure was 19 bp, and the gap sizes of the blocked strand of BF1, BF2 and BF3 were 4, 2 and ... The N and I domains of DmGEN were similar to those of XPG ⁄ class I (Fig. 2A,B). The N domain of DmGEN showed 35.1, 25.0 and 10.9% homology (% identity) with the N domain...

Ngày tải lên: 07/03/2014, 10:20

... design and use of multivalent ligands and inhibi- tors. Angew Chem Int Ed 37, 2754–2794. 38 Barbar E, Kleinman B, Imhoff D, Li M, Hays TS & Hare M (2001) Dimerization and folding of LC8, ... p53–p21 and JNK pathways [91]. LC8 interacts with the transcription factor TRPS1 and suppresses its transcriptional repression activity [92]. LC8 binds to the N-terminal disordered d...

Ngày tải lên: 14/03/2014, 22:20

... biochemi- cally, and to determine the structure of RMD to better understand what determines substrate specificity and catalytic activity in these enzymes. We used capillary electrophoresis and NMR analysis of ... RMD monomer. The cofactor-binding domain and the substrate- binding domain are shown in aqua and light sand, respectively; the APPR portion of the cofactor (dark g...

Ngày tải lên: 16/03/2014, 01:20

... participates of H 2 O 2 -mediated signaling proces- ses, including regulation of gene expression [27–30]. Therefore, we have evaluated possible influences of cTPxI and of the functional state of the ... presence of H 2 O 2 and thereby it interacts with the target genes [11,46,47]. This retention is dependent of the oxidation of Yap1 cysteine residues by H 2 O 2 , that modifi...

Ngày tải lên: 16/03/2014, 14:20

... group of GGGP and GGPP. These facts strongly suggest the involvement of the enzyme, A. fulgidus GGR, in the biosynthesis of membrane lipids. Results Recombinant expression and purification of an ... volume of 100 lL, 430 pmol of [1- 14 C]IPP (2.1 GBqÆmmol )1 ), 1 nmol of (all-E) farnesyl diphosphate, 0.2 lmol of a-glycerophosphate, 2 lmol of MgCl 2 ,2lmol of sodium ph...

Ngày tải lên: 30/03/2014, 09:20