Báo cáo khoa học: Microfibril-associated glycoprotein-1 binding to tropoelastin doc

... in quadruplicate. Results MAGP-1 binding to tropoelastin is dependent on divalent cations Solid-phase binding assays were used to determine the effect of metal ions on the binding of MAGP-1 to tropoelastin. Strong binding ... present Concentration (m M ) k D (n M ) Tropoelastin Calcium (II) 1 2.8 ± 0.3 Tropoelastin Calcium (II) 0.5 Binding occurred Tropoelastin Calci...

Ngày tải lên: 30/03/2014, 15:20

... the physio- logical roles of cytoplasmic inhibitors has been reported. I C , carboxypeptidase Y (CPY) inhibitor, was isolated as an endogenous cytoplasmic inhibitor of vacuolar CPY in the yeast ... (Met1–His18) in its carboxypeptidase Y -binding sites is involved in the specific and efficient binding to anionic phospholipid membranes. The binding of I C to cellular membranes was subseq...

Ngày tải lên: 19/02/2014, 05:20

... stopped by the addition of 10% (v ⁄ v) TCA. The radioactivity incorporated into newly synthesized RNA was then determined. To quantify and analyze the 32 P-labe- led RNA, the synthesis was stopped ... in vitro RNA synthesis directed by the HCV NS5B. To assess whether the low level of RNA synthesis was related to the binding of these templates to the HCV RdRp, we performed gel shift...

Ngày tải lên: 20/02/2014, 01:20

... that is nonpermissive for ATP binding. The resulting ADP -binding state of TAP2 and the C-terminal tail of NBD1 allow binding of ATP to TAP1. Peptide binding leads to a change in the conformation ... between the two subunits when compared to the wild-type transporter. Thus, binding of ATP to the chimeric TAP2 chain seems to interfere with ATP binding to TAP1, presumably...

Ngày tải lên: 20/02/2014, 02:21

... reduces molecular oxygen to water and couples the free energy of this reaction to the generation of a transmembrane proton gradient. The mitochondrial enzyme consists of up to 13 subun- its, of which ... exact cofactor- binding stoichiometries and enzymatic mechanism is still unresolved [4–6]. With its eight transmembrane helices, heme a synthase is supposed to provide two different...

Ngày tải lên: 06/03/2014, 00:21

... mutants, the ability of these mutants to bind ssDNA was expected to be similar to that observed for the other mutants. To con- firm this hypothesis, the DNA -binding activity of each mutants was ... W71F or W165A mutants was not due to defects in the DNA -binding activities of these mutants. DNA -binding activities of Mhr1 mutants To examine the effects on DNA -binding activity...

Ngày tải lên: 06/03/2014, 09:22

... antioxidant iron che- lator deferoxamine of PKA, localized in the mitochon- drial matrix [21], promoted CREB binding to the mtDNA D-loop. The presence in mitochondria of the CREB factor and, in particular, ... membrane import complex. Addition to the mitochondrial import mixture of an antibody against Tom20, an outer membrane receptor of the mitochondrial import sys- tem [23], reduced t...

Ngày tải lên: 07/03/2014, 02:20

... on its oper- ator DNA in vitro without effector recognition or ATP binding. The HbpR protein even seems to be able to form an oligomeric complex capable of protecting the operator DNA in the ... each protomer contacts the DNA. The objectives of this work were to identify critical motifs in the binding site for HbpR and to examine the necessity of the sensing A-domain of HbpR for DN...

Ngày tải lên: 07/03/2014, 17:20

Ngày tải lên: 08/03/2014, 18:20

... was reported to accompany the binding of the lac repressor to nucleo- somal DNA [32]. Similar to this, we have observed an asymmetric DNA deformation when R3 binds to the lac operator only on ... nuclease-hypersensitive regions and DNA distortions are reported to be important factors for nucleosome positioning [41,42]. Binding of the lac repressor to DNA has been studied in gr...

Ngày tải lên: 16/03/2014, 10:20