Báo cáo khoa học: Analysis of ancient sequence motifs in the H+-PPase family doc

... in the highest yield, and they all were among the amino acids found in the Murchison mete- orite [11]. Both the high content of the ‘very early’ amino acids in these sequence motifs, and the ... along the protein chain (Fig. 3). From the resulting plot it is clear that most of the con- served residues are located on the cytosolic side of the J. Hedlund et...

Ngày tải lên: 30/03/2014, 10:20

... carbonyl of Asn by the nitrogen of the peptide group linking the Asn to the following residue [4–6] 2 . As hydrolysis of this intermediate may occur on either side of the imide nitrogen, the Asp ... containing the regulatory and catalytic domains [15]. Fig. 2. Stereo picture of the three Asn residues in the N-terminal autoregulatory sequence of rPAH. Figure b...

Ngày tải lên: 08/03/2014, 08:20

... novel sequence motifs in the p26 N-terminal extension and another in the C-terminus. The deletion of these motifs, termed G (multiple glycine), R (multiple argin- ine), and TS (multiple threonine ... one enriched in glycine and the other in arginine. Addition- ally, the C-terminus contains an unusual stretch of residues endowed with threonine and serine. These regio...

Ngày tải lên: 30/03/2014, 20:20

... mutant proteins. This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG is not ... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein po...

Ngày tải lên: 19/02/2014, 13:20

... by the C domain of the initiation module of dapto- mycin. That DptE and DptF are involved in the lipidation process of daptomycin was first shown by Miao et al. [1]. In their work, the daptomycin ... is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence. To determin...

Ngày tải lên: 07/03/2014, 04:20

... of the MR151- derived strain with the crtIa deletion (MR841) com- pletely lose the red color typical of colonies from the parental strain, suggesting that the absence of CrtIa blocks the synthesis ... compilation ª 2007 FEBS 4307 in- frame deletion of crtIa in M. xanthus strain MR151. This strain contains a mutation, carR3, which renders the expression of the carB...

Ngày tải lên: 16/03/2014, 05:20

... have been identified in those strains that are responsible for most of the steps in the biosynthesis of the oligosaccharide portions of the LPS molecules. The inner core of H. in uenzae LPS has ... from the nasopharynx to the middle ear via the eustachian tube, often in the presence of viral respiratory infections [5]. Whether all colonizing strains of H....

Ngày tải lên: 16/03/2014, 16:20

... are the pH and E h7 in the experiments with the addition of inhibitor (the arrow indicates the time of addition); curves (C) and (D) are the pH and E h7 in the control experiment. Thiol groups in ... addition of NEM or AMdiS to a reducing culture of L. lactis rapidly increased the E h7 and restored the initial E h7 value (Fig. 3). These data show the implicati...

Ngày tải lên: 22/03/2014, 21:21

... sequence of OPN and the N-terminal sequence of dentin matrix acidic phos- phoprotein 1 (DMP1) [27,28] and long sequences of Glu residues in, for example, integrin-binding sialo- phosphoprotein ... sequences found in SPs with known involvement in mineralization are shown in Table 1, and aligned sequences of their orthologues are given in Doc. S2 (see Supporting infor- m...

Ngày tải lên: 29/03/2014, 23:20

... determined using 25 n M [ 3 H]PonA and increasing amounts of proteins in individual incubations. The percentage of specific b inding increased in a protein concentration-dependent manner within the range of ... fractions with increasing concentrations of [ 3 H]PonA, then subjecting the binding data to Scatchard analysis (Fig. 4). The analysis showed the presence of...

Ngày tải lên: 30/03/2014, 15:20