Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx

... from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane- intrinsic prenyltransfer- ase involved in the biosynthesis of archaeal ether-linked membrane ... These facts strongly suggest the involvement of the enzyme, GGR, in the biosynthesis of membrane lipids in the hyper- thermophilic archaeon...

Ngày tải lên: 30/03/2014, 09:20

... [63]. In the fish, Cyprinus carpio,an increase in GS was observed after several days of expo- sure to cypermethrin. At the same time, there was a decrease in the amount of free amino acids coincident with ... also present in the herbicide SSH lib- raries (including glutamine synthetase and cathepsin). In our experiments, we tested a cocktail of the three more common h...

Ngày tải lên: 30/03/2014, 15:20

... design of and screening for small binding proteins. The preparation of the small binding proteins with binding function further allows us to increase the binding strength by multi- binding approaches, ... loop of antibody fragments. (A) Replacement of the candidate location in NCS for grafting with the CDR 3 loop of VHH. (B) Insertion of the CDR 1-containing loop of...

Ngày tải lên: 06/03/2014, 11:20

... biochemical purification of specific peptide sequences, and in some cases the subsequent cloning and sequencing of the corresponding gene or cDNA. Only in the case of the human defensins have genes for ... be involved in the regulation of expression of pleurocidin (and possibly other antimicro- bial peptide) genes. Little is known about the promoter or regulatory s...

Ngày tải lên: 31/03/2014, 07:20

... footnote) in the reaction mixture may reduce a proportion of the FMN domain, converting some of the enzyme to the active form. The  3-fold increase in activity may arise from the binding of the FMN ... for the surface of the CPR FMN domain (in the region of the solvent- exposed FMN) with that of a homology model of the MSR FMN domain (based on the...

Ngày tải lên: 18/02/2014, 08:20

... Importin a possesses two major domains for its adaptor function, the importin b binding (IBB) domain in its N-terminus and the C-terminal NLS- binding domain. In the absence of importin b,an auto-inhibiting ... b,an auto-inhibiting part of the IBB domain forms an intra- molecular interaction with the NLS-binding domain, preventing the association with NLS on the cargo pr...

Ngày tải lên: 15/03/2014, 11:20

... binding of Sin3 to the G 2 ⁄ M-specific CLB2 promoter [50]. Their data further showed that the recruitment of Sin3 is dependent upon an interaction with Fkh2 and Mcm1. The removal of Sin3 and the deacetylase ... [48] in the promoter regions of CLN1 and CLN2. Any one of the proteins present at the CLN2 promoter in early G 1 could, in principle, be responsible for r...

Ngày tải lên: 23/03/2014, 05:22

... interaction is not observed in the crys- tal structure. In addition, the stabilizing interactions of the lysine with the carbonyl groups in the glycine rich loop in the neighbouring protomer are not ... abolished. Inspection of the crystal structures indicates that the side chain of K109 plays a dual role by forming a salt bridge to D137, as well as stabilizing a...

Ngày tải lên: 30/03/2014, 01:20

... remit of the local ethics committee. Prior to introduction of CPOE, local standards of prescribing existed specifying the tenets of good practice, including the avoid- ance of the use of abbreviations. An ... Most of the errors were minor in outcome, although two non-inter- cepted errors with CPOE led to an increased length of stay or increased monitoring. In th...

Ngày tải lên: 25/10/2012, 10:39

... use the erythropoietin receptor (EpoR)-induced activation of ERK1 in the hematopoietic suspension cell line, BaF3-hemaglutinin-tagged (HA)-EpoR, and the interleukin-6 (IL-6)-induced activation of ... weak affinity to the protein, cross-reaction with other proteins resulting in a high background, or by the use of generic phosphotyrosine antibodies. In such cases, the pro...

Ngày tải lên: 19/02/2014, 07:20