Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt
Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt
... fujikuroi by a point mutation in the phytoene desaturase gene Alfonso Prado-Cabrero 1 , Patrick Schaub 2 , Violeta Dı ´ az-Sa ´ nchez 1 , Alejandro F. Estrada 1 , Salim Al-Babili 2 and Javier Avalos 1 1 ... manufacturer’s instructions. Two microlitres of cDNA were used for the amplification of carB using the primers 5¢-ATGAGCGACATTAAGAA ATCTG-3¢ and 5¢-CTAATTCGCAGCA...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx
... reverse CTCGCGGGCTCGGCAGTGGGAG P prom+1 AGTCTATTCTCGAGCACCTGGGACTACAGG P prom+2 AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG P prom+3 AGTCTATTCTCGAGATGCTTCTGGAGTAGGAGGCA P prom+4 AGTCTATTCTCGAGGTGTGGAGGAGGCAGGGAGAC P prom+5 AGTCTATTCTCGAGGAGGCGCTCTGCAGTGCCTC P prom+6 AGTCTATTCTCGAGAAGCAGGACGTTCCCACGCTG P prom+7 AGTCTATTCTCGAGGGGGCGGGCGCCCGCACTCAG P promreverse AGTCTATTCTCGAGCGCGACCTGGGTCTGCCCAG P ORE...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc
... 1 5¢)GGGGAGCTCTTAGAGAATGACAACATATGG-3¢ pPICZaA5¢cloning primer mature Der p 1 5¢)GGGCTCGAGAAAAGAACTAACGCCTGCAGTATCAAT-3¢ Sequence primers used for vector pPICZaA5¢AOX, 3¢AOX and a- factor primer 672 ... (data not shown). Proteolytic cleavage of recombinant pro-Der p 1 As autocatalytic cleavage was not achieved, enzymatically active natural Der p 1 was evaluated as a tool to induce matura...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand pptx
... TACAATGTACC GGAGATCTATTGATC ATGTTACATGGCCTCTAGATAACTAG 3 TACAATGTACC GAAGATCTATTGATC ATGTTACATGGCTTCTAGATAACTAG 4 TACAATGTACC GTGGATCTATTGATC ATGTTACATGGCACCTAGATAACTAG 5 TACAATGTACC GAGAATCTATTGATC ATGTTACATGGCTCTTAGATAACTAG 6 TACAATGTACC GAmAGATCTATTGATC ATGTTACATGGCTTCTAGATAACTAG 7 ... TACAATGTACC G2GGATCTATTGATC ATGTTACATGGCTCCTAGATAACTAG 10 TACAATGTACTC GAAGCTATCTATTGATC ATGTTACATGAGCTTC...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx
... that the cell-bound and -generated FXIIa and kallikrein are protected from inactivation by endogenous inhibitors in the plasma. Activation of PPK plays a primary role in initiating and maintaining ... cleavage product of HK generated by proteolytic attack by kallikrein generated by activation of PPK as a consequense of vessel injury. The activation of FXII and...
Ngày tải lên: 23/03/2014, 20:22
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... 3¢-GTCACAGAGCCACAGTTCCAG CCAGGA-5¢. Codon 305 of C-YES was mutated from AAA (Lys) to AGA (Arg) with the forward primer: 3¢-GG AACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts ... 96-well plate format assay. Each sample contained 2.5 lg of total protein. Values are the average of four samples with error bars indicating the SEM. Phosphorylation of C...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... enhanced in the presence of IRE Binding to RNA and ATPase activity are characteris- tics of RNA helicases, and RNA binding increases the ATPase activity of these proteins [26]. To test whether the ... IRP-1, again increasing IRE-binding activity. It is proposed that the H 2 O 2 -mediated conver- sion from cytosolic aconitase to an IRE-binding protein is a result of a sig...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... a major contaminant of eEF 1A protein can be excluded by MALDI TOF analysis of the Coomassie blue band extract [21] and by the absence of a corresponding nucleolin signal in western-blotting of ... oligodeoxy- nucleotides interacting with the SH2 domain of Stat3, a protein encoded by a proto-oncogene that is activa- ted in many human cancer cells, represent...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Mechanisms of amyloid fibril self-assembly and inhibition Model short peptides as a key research tool pptx
... crucial role in the fibrillization process [27]. The results of a systematic alanine-scan of a shorter IAPP fragment (Table 1) indicated that other than phenylalanine, any amino acid within the fragment could ... important informa- tion of the role of aromatic moieties in amyloid fibril formation [36–41]. A parameter-free model based on the mathematical analysis of...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo khoa học: Folding of epidermal growth factor-like repeats from human tenascin studied through a sequence frame-shift approach pdf
... 102030405060 0102030405060 area (%)area (%) time (min) A Fig. 4. Oxidative folding kinetics. (A) Disappearance of the starting product (%, area of the initial reduced form with respect to the total integrated area) ... repeats display- ing a different pattern in the cysteine spacing. The oxidative folding of frame-shifted peptides simulates, in a way, the mispairin...
Ngày tải lên: 23/03/2014, 13:20