Báo cáo " RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY " potx

... 2011 161 RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY Nguyen Thi Thu Huong (A) Ho Chi Minh City University of Technology (Hutech) TÓM TẮT Protein tái ... literature review will outline some aspects of animal cell technology regarding the production of recombinant proteins. PROTEIN EXPRESSION SYSTEMS Recombinant...

Ngày tải lên: 28/03/2014, 09:20

... Moran, and Fernando Perelm for helpful discussion and comments. CHAT (Pereira 1983), and the representations are based on those used in that system. We shall show that an analysis of tense and ... president and she is his cur- rent wife • He is the current president and she was his wife in 1975 • He was the president in 1975 and she was his wife then (but perhaps he is...

Ngày tải lên: 21/02/2014, 20:20

... morphology and dimensions of the cells were microscopically determined and compared with a standard. Cellular protein content The protein concentration of the cells was determined using the Robinson–Hogden ... of cells (10 6 cells·L –1 ) b: bud or daughter m: mother cell cell Fig. 2. Cell- size distribution (A) and morphology changes during long-term pH-auxostat cultivation at s...

Ngày tải lên: 16/03/2014, 04:20

... 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢. Direct measurements of [Ca 2+ ] i in single cells Non-transfected HEK293 cells and HEK293 cells stably transfected with the 5-HT 7(b) receptor (KB1 cells) ... signalling in HEK293 cells, we first used immunoprecipitation analysis and RT-PCR to detect whether Ras-GRF1 protein and mRNA, respectively, were expressed in our...

Ngày tải lên: 19/02/2014, 18:20

... protein expression for 24 h. Unbound ligand was removed by washing the cells with NaCl ⁄ P i , and the cells were incubated for 1 h in the F-12 medium containing 10% fetal bovine serum. Some cells ... induced 17b-HSD11 protein in the jejunum but not in the colon, and to a much higher extent than in the liver of mice. A subcellular localization study using Chinese hamster ovary cells...

Ngày tải lên: 07/03/2014, 05:20

... m M . Recombinant (j)andnative(h)GAPDH(1.5· 10 )9 M and 6 · 10 )9 M , respectively) were placed in the assay cuvette and the appearance of product was monitored. Mean rate constants and standard ... Both proteins were dialyzed in buffer B and concentrated together to a final volume of 50 lL. After concentration, 10% glycerol was added and the proteins were incubated 45 min at 30 °C...

Ngày tải lên: 23/03/2014, 20:22

... LNCaP cells. Influence of TPD52 overexpression on LNCaP cell migration Furthermore, we analyzed whether overexpression of EGFP-TPD52 fusion protein affects LNCaP cell migration. Haptotactic cell ... overexpression on the proliferation of the PCA cell line LNCaP. (A) Cell proliferation after overexpression of TPD52 and (B) cell proliferation after downregulation of TPD52. Viabil...

Ngày tải lên: 30/03/2014, 02:20

... binding protein; FABP3, heart-type fatty acid-binding protein; CRBP, cellular retinol binding protein; CRABP, cellular retinoic acid binding protein; ILBP, intracellular lipid binding protein; ... oocytes and liver. Keywords: FABP gene; oocyte; tissue-specific expression; cis element; linkage mapping. Intracellular fatty acid-binding proteins (FABP) and the related cellular retinol...

Ngày tải lên: 31/03/2014, 07:20

... measurement of HLA-DR expression in permeabilised cells measures both intracellular and surface. A comparison to the surface value allowed an estimate of intracellular versus extracellular expression ... Supernatants from the cell cultures with GM-CSF were stored at –70ºC and assayed for sHLA-DR as described earlier. Western Blotting for sHLA-DR in cell lysates and plasma...

Ngày tải lên: 03/11/2012, 09:57

... selection of transformants. Protein techniques Protein was determined by protein- dye binding with BSA as a standard [47]. Whole cell protein of C. ammoniagenes cells was isolated from 2 mL of induced ... with high protein content, resulting in atomization temperatures of 2300 °C and 2150 °C for manganese and iron, respectively. L-tryptophan-containing standards were used to si...

Ngày tải lên: 15/02/2014, 01:20