Báo cáo khoa học: Calpain 1–titin interactions concentrate calpain 1 in the Z-band edges and in the N2-line region within the skeletal myoﬁbril doc

... 2578–2590 ª 2005 FEBS Calpain 1 titin interactions concentrate calpain 1 in the Z-band edges and in the N2-line region within the skeletal myoﬁbril Fabrice Raynaud 1 , Eric Fernandez2, Gerald Coulis2, ... Thus,according to these and previous results [20, 21] , titinappears to be a calpain carrier that concentrates cal-pain 1 and 3 in the N1- and N2-line region. The pres-ence of calpain 1 in the vicinity ... Consequently, the colocalization of calpain 1 and calpain 3 at N1- and N2-line levels, the postmortem fractures at the N2-line level, as well as the implication of titin in the bindingto calpain 3 [20] and...

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... 6G155K, D (15 6 15 7), A158E 66 ± 12 73 ± 6*M149K, G155K, D (15 6 15 7), A158E 58 ± 5 15 6 ± 13 *N152D, G155K, D (15 6 15 7), A158E 62 ± 8 76 ± 7*M149K, N152D, G155K, D (15 6 15 7), A158E 93 ± 16 14 8 ± 17 ** Signiﬁcantly ... this region, adapted from [2].Residue 14 7 14 8 14 9 15 0 15 1 15 2 15 3 15 4 15 5 15 6 15 7 15 8 15 9 16 0 17 0 17 1 17 2Consensus – G K I – DELLV––––VLID––TPAI -1 K G M I S N L L G K G A V D Q L Ta 1 PI ... ± 2S129A 80 ± 17 59 ± 8E130A 59 ± 4 53 ± 3V131A 74 ± 13 70 ± 4E132A 51 ± 9 30 ± 1* R133A 90 ± 10 57 ± 2R135A 57 ± 6 26 ± 2*F136A 88 ± 12 67 ± 5I137A 18 ± 2 < ;10 and 17 0 ± 10 *aI138A...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... RNA–protein interactions in RNA foldingFEBS Journal 278 (2 011 ) 16 34 16 42 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 16 37Tat peptide induced a change in the population ofcoexisting and interchanging ... http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms(Received 23 November 2 010 , revised 8February 2 011 , accepted 11 March 2 011 )doi :10 .11 11/ j .17 42-4658.2 011 .08094.x The RNA folding trajectory ... annealing of the new duplex ﬁnally results in either the replacement of the original strand or the expulsion of the invadingstrand, according to the kinetics and thermodynamicsituation. If the...

Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

... Heath JK (19 92) Involvementof AP1 and PEA3 binding sites in the regulation ofmurine tissue inhibitor of metalloproteinases -1 (TIMP- 1) transcription. Biochim Biophys Acta 11 71, 41 55.38 Ulisse ... &Narumiya S (19 96) Rhotekin, a new putative target forRho bearing homology to a serine ⁄ threonine kinase,PKN, and rhophilin in the rho-binding domain. J BiolChem 2 71, 13 556 13 560.Rac signaling towards ... ERK1,2signaling in Tiam1 ⁄ Rac-induced and AP -1- mediatedupregulation of TIMP -1, we investigated whether Tiam1 ⁄Rac-induced binding of nuclear extracts to the AP -1 siteat )92 ⁄ )86 of the...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... 7.4, containing 13 5 mm NaCl) supple-mented with a mixture of protease inhibitors (10 0 lgÆmL )1 PMSF, 2 lgÆmL )1 leupeptin, 1 lgÆmL )1 pepstatin A, and 1 lgÆmL )1 p-toluenesulfonyl-l-arginine methyl ... for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro. Cell 57, 11 09 11 22. 18 Holgado-Madruga M, Emlet DR, Moscatello DK,Godwin AK & Wong AJ (19 96) A Grb2-associateddocking ... Osaka573- 010 1, JapanFax: + 81 72 866 311 7Tel. + 81 72 866 311 8E-mail: takeuchi@pharm.setsunan.ac.jp(Received 31 March 2006, revised 3 June2006, accepted 14 June 2006)doi :10 .11 11/ j .17 42-4658.2006.05377.xWe...

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea ﬂexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

... (0.99) 17 7 (0.93) 19 6 (0.98) 19 2 (1. 01) 16 9 (0.93) 203 (0.98) 17 0 (0.94) 16 9 (0.93)main-main 11 6 (0.59) 11 8 (0.62) 12 1 (0. 61) 11 7 (0.60) 11 5 (0.59) 11 7 (0.60) 11 7 (0.62) 10 9 (0.59) 11 6 (0. 61) 10 8 ... 19 4 19 0 18 5 19 0 17 8 18 2 18 1 18 4 207Hydrogen bonds 19 1 (1. 00) 17 9 (0.94) 200 (1. 01) 19 9 (1. 03) 19 6 (1. 01) 19 2 (0.99) 18 3 (0.96) 18 0 (0.97) 18 8 (0.99) 17 0 (0.94) 17 7 (0.97) 17 0 (0.94) 17 9 (0.97) ... (0. 61) 11 0 (0.60) 10 6 (0.59) 11 1 (0.60) 12 6 (0. 61) 11 8 (0.62) 11 9 (0.60) 12 0 (0.63) 10 6 (0.59) 12 7 (0. 61) 10 6 (0.59) 10 6 (0.59)main-side 28 (0 .14 ) 26 (0 .14 ) 39 (0.20) 43 (0.22) 43 (0.22) 36 (0 .19 )...

Báo cáo khoa học: A Caenorhabditis elegans model of orotic aciduria reveals enlarged lysosome-related organelles in embryos lacking umps-1 function potx

... 5¢-TCAAAATTGGTGTGAATGACGAGAAAC-3¢ and P598. The resulting F11E6 .1: :mCherry product was coinjected into N2at 20 ngÆlL )1 with pRF4[Rol-6D] at 10 0 ng ÆlL )1 . kxEx148[F11E6 .1: :mCherry; rol-6(su1006)D] was used in the ... pyr -1( RNAi)f 10 0 73umps -1( RNAi); pyr -1( cu8)f00 0 31 umps -1( zu456); pyr -1( cu8)m00 0 40umps -1( zu456); R12E2 .11 (RNAi)f 10 0 0 0 31 Mosaic RNAirrf -1( pk14 71) n00 0 30rrf -1( pk14 71) ; umps -1( RNAi) 69 0 ... 5¢-TATCTTCTTCACCCTTTGAGACCATGTAGTTGTCATCGTAGACGTGGC-3¢. The genomic region containing 14 00 bp of sequenceupstream of the predicted start codon of F11E6 .1 and the entire predicted coding sequence predicted to include the 3¢-end of splice forms F11E6.1a...

Báo cáo khoa học: Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment pptx

... regarding the molecular mechanisms underlying the detrimental role of the enzyme in ischemic braininjury [ 31, 32]. Indeed, although we know in part the mechanisms activated by PARP -1 and triggeringneurotoxicity, ... Metab 19 , 819 –834. 17 Iadecola C & Alexander M (20 01) Cerebral ischemia and in ammation. Curr Opin Neurol 14 , 89–94. 18 Wang X & Feuerstein GZ (2004) The Janus face of in ammation in ischemic ... integrityMMPMMP 1- PRAP 1- PRAP 1 -PRAP 1- PRAP 1- PRAP 1 -PRAPFIAFIATRPM2aC+21BGMHHMGB 1 yrotammalfnIsrotaidemyrotammalfnIsrotaidemXXXXMMPyrotammalfnIsrotaidemnorueNMicrogliaetycortsAetycokueLmuilehtodnEnemuLBasal laminaFig. 1. The role of PARP -1 within the ischemic neurovascular units. PARP -1 exerts its detrimental role within the ischemic neurovascularunit by promoting necrosis and AIF-dependent...

Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

... relativelylow, the N-terminal region (aN–a1), the aF–aG region and the C-terminal region (a4–a5) were well conservedbetween GOA -1 and Gao[ 31, 32]. The ﬁve regions of the Ga-subunit involved in receptor ... GTPcS in GOA -1. The IC50value of the displacement of GTPcS by GDP was3 · 10 )6m with 0.2 mm atropine, and 10 )4m withoutatropine in the presence of 1 mm carbamylcholine,which induced the ... ocoupling, suggestingthat the function of GOA -1 and Gai ⁄ owas evolutio-narily conserved in the coupling with M2.These results also indicate that the distinct aminoacids in GOA -1 are...

Báo cáo khoa học: Leukocyte–endothelial cell interactions pot

... activation, resulting in a transfer ofleukocyte integrins into a high afﬁnity conformation. The integrins now allow the leukocytes to adhere ﬁrmlyto the endothelium by interactions with intercellularadhesion ... smallGTPases, reactive oxygen species and protein kinases,are involved in these signaling events.doi: 10 .11 11/ j .17 42-4658.2006.05436.xFEBS Journal 273 (2006) 4375–4376 ª 2006 The Authors Journal compilation ... molecule -1 and ⁄ or vascular cell adhesionmolecule -1. The leukocytes then migrate to sites wherethey can cross the endothelial layer, using either a para-cellular pathway through intercellular...

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