Báo cáo khoa học: Functional dissection of a small anaerobically induced bZIP transcription factor from tomato pdf

... and5¢-ATATCCATGGAAAATTTAAACAATCCTGATG-3 ¢.Abz1(1–44): 5¢-TATACTCGAGATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCATGGGCTTCTTCATCCTCGATCGC-3¢. Abz1(1–24): 5¢-TATACTCGAGATGTCACCTTTAAGGCAGAG-3¢ and 5 ¢-ATATCCATGGTCTCATCCATTCCTGCATAC-3¢. ... 5¢-TATGGATCCATGCTTCTGCAAGATTTGACAGG-3¢ and 5¢-ATATCCCGGGTTAAAATTTAAACAATCCTG-3¢.C-terminaldeletion, ABZ1(1–100): 5¢-TATAGGATCCATGTCACCTTTAAGGCAGAG-3¢ and 5¢ -A TACCCGGGTTATAAATACCTGAGCCTATCAGTC-3¢.The BamHI and SmaI sites ... pairs. Full size, ABZ1(1–138): 5¢-TATAGGATCCATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCCGGGTTAAAATTTAAACAATCCTG-3¢.N-ter-minal deletion, ABZ1(47–138): 5¢-TATGGATCCATGCTTCTGCAAGATTTGACAGG-3¢ and...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... 5¢-AAGCTTCACCATGTACCCTGCCCACATGTACCAAGTGTAC-3¢,5¢-AAGCTTCACCATGCCGCACCGG CTCATCGAGAAAAAGAG-3¢,5¢-AAGCTTCACCATGGCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGGTTTACAAGCTG-3¢) were used. Ampliﬁed ... and5¢-GCAGCAGGATCCTCTAGAGAGTTTAGTCTTTG-3¢ for FLAG-DEC1; and 5 ¢-GAATTCGGCGGACCAGAGAATGGACATTTCCTCAACCATC-3¢ and5¢-TCTAGACTACAGCGGCCATGGCAAGTCACTAAAGTC-3¢ for FLAG-BMAL1) were used for ampliﬁcationby ... primers(5¢-CGGCAATTTGTAGGTCTCCTTGCTGTCCTCGCTC-3¢ and 5¢-GCCCGGCTCATCGAGAAAAAGAGACGTGACCGG-3¢ for DEC1-H5 7A; 5¢-GATGAGCCGGTGCGGCAATTTGTAGGTCTCC-3¢ and 5 ¢-GAGAAAAAGAGAGCTGACCGGATTAACGAGTGC-3¢ forDEC1-R6 5A, FLAG-DEC1-R6 5A and DEC1:4–232-R6 5A; ...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... 2847 Functional dissection of theSchizosaccharomyces pombeHollidayjunction resolvase Ydc2:in vivorole in mitochondrial DNAmaintenanceBarbara Sigala and Irina R. TsanevaDepartment of Biochemistry ... and maintenance of DNA in allorganisms including mitochondrial DNA (mtDNA).Mitochondrial DNA amounts to about 15% of the DNAcontent in Saccharomyces cerevisiae. A haploid cell containsabout ... total cellular DNA were loaded on the gels, as thehybridization signal in these samples was very low. Thegeneral pattern remained the same: the 19.4-kb band wasweak and a signiﬁcant amount of...

Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

... of acs: delacs P1(forward), 5¢-GAGAACAAAAGCATGAGCCAAATTCACAAACACACCA TTGTG TAGGC TGGAGCT GCTTC G-3¢;and delacs P2 (reverse), 5¢-GGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCATATGAATATCCTCCTTA-3¢. ... Pathways of acetate activationand production in E. coli. Acs catalyzes anirreversible pathway for high-afﬁnity acetateactivation, and AckA and Pta catalyze a reversible pathway involved in acetateproduction ... phosphoenolpyruvate(PEP).Acetyl-CoAAcetatePtaAcetyl-AMPAcetyl-PAckAcsAcsPiCoAADPATPAMPCoAPPiATP2PiPPasaGlucosePEPPyruvateAcetyl-CoAAcetateCoANADHNAD+CO2Acetyl-PPtaPiCoAPiCoAADPATP+-Acetate excretionAcetate assimilationABFig. 1. (A) Pathways...

Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the ﬁbronectin-binding adhesins ﬁbronectin-binding protein A and ﬁbronectin-binding protein B from Staphylococcus aureus pot

... nm bandpass ﬁl-ter). Data were recorded and analyzed with flowmax soft-ware from Partec.Statistical analysis of ELISA experimentsEach experiment was repeated at least twice, with duplicateor ... aureus, one of the most importantGram-positive pathogens of humans and animals, is a highly versatile bacterium capable of causing a widespectrum of diseases, ranging from superﬁcial skininfections ... Visai1,2, Fiona M. Burke3, Joan A. Geoghegan3,Matteo Stravalaci4, Marco Gobbi4, Giuliano Mazzini5, Carla Renata Arciola6, Timothy J. Foster3andPietro Speziale11 Department of Biochemistry,...

Báo cáo khoa học: Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily ppt

... as a template with thefollowing primers : R450AUp (5¢-GACGAGTACACGGTGGCCGGATACAACCTCAGGA-3¢) and R450ALo (5¢-TCCTGAGGTTGTATCCG GCCACCGTGT ACTCGTC-3¢);Y452AUp (5¢-ACACGGTGCGCGGAGCCAACCTCAAGACGTC-3¢) ... (5¢-ACACGGTGCGCGGAGCCAACCTCAAGACGTC-3¢) and Y452ALo (5¢-GACGTCCTGAGGTTGGCTCCGGCCACCGTGT-3¢); RA+YAUp (5¢-ACACGGTGGCCGGAGCCAACCTCAGGACGTC-3¢) and RA+YALo (5¢-GACGTCCTGAGGTTGGCTCCGGCCACCGTGT-3¢). After mutagenesis and ... analyses of FhaCWThave revealed different behaviours at posi-tive and negative potentials, with noisier current andthe appearance of conductance substates at negativepolarity (Fig. 5A, part...

Báo cáo khoa học: Functional dissection of two Arabidopsis PsbO proteins PsbO1 and PsbO2 doc

... thaliana O1Arabidopsis thaliana O2Nicotiana tabacumSolanum tuberosumLycopersicon esculentum Pisum sativum Oryza sativa Spinacia oleraceaVolvox carteriChlamydomonas reinhardtiiEuglena ... O2Nicotiana tabacumSolanum tuberosumLycopersicon esculentum Pisum sativum Oryza sativa Spinacia oleraceaVolvox carteriChlamydomonas reinhardtiiEuglena gracilisBigelowiella natansAnabaena ... quenching of chlorophyll ﬂuorescence. Plant Cell Physiol 40,1134–1142.10 Murakami R, Ifuku K, Takabayashi A, Shikanai T,Endo T & Sato F (2002) Characterization of an Arabi-dopsis thaliana mutant...

Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... absorption bands. Heme catabolism byHOs of mammals, pathogenic bacteria, cyanobacteriaand probably insects is considered to have a similarmechanism, because the characteristic absorptionbands of verdoheme ... T, Zhang X, Sun D,Sato M, Sasahara M, Kayama T, Ikeda-Saito M &Yoshida T (2000) Histidine 20, the crucial proximalaxial heme ligand of bacterial heme oxygenase Hmu O from Corynebacterium ... Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation of the reaction. The spectral features of the ﬁnal reaction mixture were analogous, but not iden-tical,...

Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

... GCGCGGATCCACCATGCCCTTCCGGAGAAGA 468/156(p26-192Xho-as) CGCGCCTCGAGTTAAGCTGCACCTCCTGATCTp26-ND60 1–60 (p26-60Bam-s) GCGCGGATCCACCATGTCCTTGAGGGACACA 396/132(p26-192Xho-as) CGCGCCTCGAGTTAAGCTGCACCTCCTGATCTp26-CD40 ... Xho-as) CGCGCCTCGAGTTATGGAGTTGAACTAGCTGTp26-alpha 1–60 and (p26-60Bam-s) GCGCGGATCCACCATGTCCTTGAGGGACACA 297/93153–192 (p26-153Xho-as) CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT934 J. A. Crack et al.(Eur. ... protein from Artemia franciscanaOligomerization and thermotoleranceJulie A. Crack, Marc Mansour, Yu Sun and Thomas H. MacRaeDepartment of Biology, Dalhousie University, Halifax, Nova Scotia, CanadaOviparously...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... (phosphocholine), 165.13 and lipid A- OH (O-deacylated lipid A) , 953.02. Relativeabundance was estimated from the area of molecular ion peak relative to the total area (expressed as percentage). Peaks representing ... ﬁltration chromatography and GLC were carried out asdescribed previously [16].Preparation of OS materialO-Deacylation of LPS. O-Deacylation of LPS wasachieved with anhydrous hydrazine, as described ... J.H. & Jeanes, A. (1965) Quantitativedetermination of monosaccharides as their alditol acetates by gasliquid chromatography. Anal. Chem. 37, 1602–1604.28. Gerwig, G.J., Kamerling, J.P. &...

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