Báo cáo khoa học: Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica) doc

... PL. Results Incorporation of exogenously injected ZP1 into quail PL To determine whether ZP1 injected intravenously into a bird was incorporated into the PL of the ovarian fol- licles, we first purified the 97-kDa ZP1 ... 545–550. M. Kinoshita et al. Incorporation of ZP1 into perivitelline membrane FEBS Journal 275 (2008) 3580–3589 ª 2008 The Authors Journal comp...

Ngày tải lên: 23/03/2014, 07:20

... bilirubin uptake into HepG2 cells depends on the activity of a membrane carrier. Bilirubin uptake into HepG2 cells: effect of a protein-modifying reagent We attempted to disrupt the integrity of ... bilirubin carrier could be identified, and possibly isolated, by applying its property to bind and transport BSP, instead of bilirubin. Initially, by applying an assay of BSP bind-...

Ngày tải lên: 07/03/2014, 21:20

... another eIF4G-binding domain within its N-termi- nal RNA-binding domain 1 [27]. As a negative control, we also examined the colocalization of b-actin with eIF4G in exponentially growing cells. As ... transfected with 2 lg of plasmid DNA using 10 lg of lipofectamine (Invitrogen, Burlington, Canada), according to protocols supplied by the manufac- turer. In short, the DNA was mixe...

Ngày tải lên: 18/02/2014, 12:20

... enzyme–inhibitor interaction was further investigated by site-directed mutagenesis of His374 into alanine, glutamine or lysine. Bind- ing kinetics and affinities of the molecular interactions between A. niger, Bacillus ... xylose. In spite of the above, profound analysis of the speci- fic contributions of the amino acid residues at the interface of the enzyme–inhibitor interacti...

Ngày tải lên: 19/02/2014, 07:20

... cell margins consistent with the role of jasplakinolide as a stabilizer of F-actin. In contrast, treatment of the cells with swinholid e A did not affect the intensity of F-actin stress fibers in the ... phosphorylation in anisomycin- treated ce lls (Fig. 10). These data are in line with previous observations indicating that physiological activators of the p38 and Ptd...

Ngày tải lên: 19/02/2014, 16:20

... nature of the substrate and the inherent partioning of quinol into the Q o site. The Fig. 3. Steady-state level of the iron–sulfur protein (ISP) in G167E. Immunoblots of denaturing gels, loaded with ... movement of the ISP by altering the ISP–cytochrome b interaction at the hinge region. Move- ment of the extrinsic domain of the ISP is facilitated by the unwinding of a s...

Ngày tải lên: 07/03/2014, 15:20

... Biotechnology Inc., Santa Cruz, CA, USA) against known transcription factors were incubated with the nuclear extract in the presence of 1 · binding buffer at room temperature for 45 min before binding ... formation of Complex A, indicating that the p65 subunit and members of the RAR family are involved in the DNA–protein Complex in TE671 cells. Intrigu- ingly, along with the a...

Ngày tải lên: 16/03/2014, 10:20

... presence of increasing concen- trations of methyl green, a majo r groove b inding drug [41] and distamycin A, a minor groove binding drug [42]. Increasing concentrations of distamycin A resulted in ... to incubate overnight. The strip was washed with three changes of 1 · binding buffer over a period of 30 min and autoradiographed. In vitro DNase I footprinting analysis DNase I...

Ngày tải lên: 16/03/2014, 18:20

... Downregulation of urokinase plasminogen acti- vator receptor expression inhibits Erk signalling with concomitant suppression of invasiveness due to loss of uPAR-beta1 integrin complex in colon cancer ... trans- fected into Saos-2 cells. Previous studies by Zhang et al. [17] investigating the stability of DNAzymes, found detectable levels of DNAzymes within 2 h of transfectio...

Ngày tải lên: 16/03/2014, 22:20

... maintained in DMEM containing 10% of fetal bovine serum (FBS; Myoclone Super Plus, Life Technologies) and 1% of penicillin/ streptomycin. The cells were infected by addition of 80 UÆmL )1 of ... mRNA insta- bility and the involvement of the ARE in this process [10,11]. In addition, another destabilizing sequence was identified within the coding region of IFN-b mRNA [12,13]. W...

Ngày tải lên: 17/03/2014, 10:20