Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx

... of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previously demonstrated by a yeast two-hybrid assay [ 7], and this interaction may be involved in the suppressive activity of DEC1 against ... reporter assay. Western blot analysis Rabbit antibodies to DEC1 were produced by immun- izisation with the synthetic peptide fragment Cys-Lys- Gly-Asp-Leu-Arg-Ser-Glu-Gln-Pro-...

Ngày tải lên: 19/02/2014, 16:20

... 6057 catalytically active PLA 2 , the membrane disorganization seems to be potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins ... Furthermore, one can speculate that DM64 binds to the myotoxins through a myotoxic/ cytolytic site distinct from the catalytic site, as already described for the inh...

Ngày tải lên: 21/02/2014, 01:21

... antibody to p26 reacted poorly with polypeptides encoded by p26-ND60 and p26-alpha, demonstrating that enhanced thermotolerance conferred by these two constructs correlated with synthesis of p26 polypeptides. ... example of stress resistance, fully hydrated cysts survive several years at physiological temperature in the complete absence o f o xygen [48,51,52,54], an unusual degree...

Ngày tải lên: 22/02/2014, 04:20

... Ig (DynabeadsÒ M-450 CD4, Dynal). After two rounds of selection, cells were cloned by limiting dilution and the clones analysed by FACS for coexpression of CCR5 and CD4. Western blot analysis and 35 S-labelled ... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected b...

Ngày tải lên: 08/03/2014, 09:20

... between phycobilisomes and the photosynthetic lamellae [6]. Both phycocyanin and allophycocyanin are composed of two polypeptide chains, a and b, of approximately 17 000 and 18 000 [7]. The a and b ... environment in diverse ways [2]. In general the macrostructure of phyco- bilisomes consists of a core (constituted by allophycocyanin) surrounded by phycocyanin and...

Ngày tải lên: 08/03/2014, 16:20

... sub- strate–enzyme interactions of the wild-type and one of the mutants. The results provide indications of the native enzymes’ catalytic action and determinants of Analysis of a b-glucosidase aglycone-binding ... any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutants was...

Ngày tải lên: 16/03/2014, 04:20

... modeled by the superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculated by deconvolution ... gov- erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail- able electron transport path...

Ngày tải lên: 19/02/2014, 05:20

... substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic functions in proximity for effective enzyme activity. Two ... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-a...

Ngày tải lên: 21/02/2014, 15:20

... proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fn may be sterically hindered in the presence of the anti- body. The results of this study allow us ... attachment of bacteria to Fn, even in the presence of excess amounts of 15E11, is mediated by repeats that are not targeted by the mAb. Interestingly, the inhib...

Ngày tải lên: 06/03/2014, 22:21

... optimum, and a broad speci®city w ith respect to the g lycone moiety of substrates. Human CBG hydrolyses synthetic aryl glycosides (including 4-nitrophenyl and 4-methylumbelliferyl monoglycosides) [9], ... [the hydrolysis of 4NP-b- D - glucopyranoside (10 m M ) was reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides b...

Ngày tải lên: 08/03/2014, 16:20