Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previouslydemonstrated by a yeast two-hybrid assay [ 7], and thisinteraction may be involved in the suppressive activity of DEC1 against ... reporterassay.Western blot analysis Rabbit antibodies to DEC1 were produced by immun-izisation with the synthetic peptide fragment Cys-Lys-Gly-Asp-Leu-Arg-Ser-Glu-Gln-Pro-Tyr-Phe-Lys-Ser-Asp-His-Gly-Arg-Arg. ... two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto the CACGTG E-box in the Dec1 promoter. Various DEC1 mutants and VP1 6-taggedDEC1 mutants were synthesized by using anin...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... 6057catalytically active PLA2, the membrane disorganizationseems to be potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins ... Furthermore, one can speculatethat DM64 binds to the myotoxins through a myotoxic/cytolytic site distinct from the catalytic site, as alreadydescribed for the inhibition of myotoxicity by heparin ... conditions. The slightly higher value given by SDS/PAGE is probablyconsequence of the glycosylated nature of DM64 [42]. The molecular mass of native DM64 was also analyzed usingdifferent gel filtration...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... antibody to p26 reacted poorlywith polypeptides encoded by p26-ND60 and p26-alpha,demonstrating that enhanced thermotolerance conferred by these two constructs correlated with synthesis of p26polypeptides. ... example of stress resistance, fully hydrated cystssurvive several years at physiological temperature in the complete absence o f o xygen [48,51,52,54], an unusual degree of tolerance for any animal. ... animal. These observations contradict the general belief that under ordinary hydration and temperature, cell maintenance entails a constant and substantial free energy flow [51,52]. Anoxic cysts m ayacquire...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... Ig(DynabeadsÒ M-450 CD4, Dynal). After two rounds of selection, cells were cloned by limiting dilution and the clones analysed by FACS for coexpression of CCR5 and CD4.Western blot analysis and 35S-labelled ... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of ... obtained by limiting dilution and analysed by FACS using the antibody 2D7 (Fig. 1), which recognizesCCR5 [36]. Western blot analysis of the cloned protein froma postnuclear supernatant of lysed...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... between phycobilisomes and the photosynthetic lamellae [6]. Both phycocyanin and allophycocyanin are composed of two polypeptide chains,a and b, of approximately 17 000 and 18 000 [7]. The a and b ... environment indiverse ways [2]. In general the macrostructure of phyco-bilisomes consists of a core (constituted by allophycocyanin)surrounded by phycocyanin and phycoerythrin (whenpresent) ... nergymigration through the phycobilisomes and to chlorophyll ain the thylakoid membrane [5]. Phycocyanin is the majorconstituent of the phycobilisomes, while allophycoc yaninholds the bridging...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... sub-strate–enzyme interactions of the wild-type and one of the mutants. The results provide indications of the native enzymes’ catalytic action and determinants of Analysis of a b-glucosidase aglycone-binding ... any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutantswas found to be irreversible (Fig. S3). The propensity of ... propensity of the wild-type and each of the single mutant enzymes to form dimers was analyzed by size-exclusion chromatography. The enzymes werepurified by metal chelate affinity chromatography and subjected...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... modeled by the superposition of the exponential rise to analyzequantitatively the contribution of each phase. The amplitude and rate constant of each of the threephases can be calculated by deconvolution ... gov-erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail-able electron transport pathways, which should be dif-ferent in isolated thylakoid ... easily explained by the fact that a nonsaturating concentration of DCMU was used, meaning that only a fraction of the PSII reaction center was affected by DCMU. Then, the intact fraction of PSII...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic functions in proximity foreffective enzyme activity.Two ... as the high-affinity peak of wild-type LPL. The LPL in the eluate wasdetected by Western blot with LPL polyclonal antibody7640. The fraction number of the high-affinity peak was the same for the ... structure of monomeric LPL was duplicated and superimposed on the crystal structure of the horse PLdimer. After the energy minimization by setting at iteration of 300 cycles or energy level of the...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fnmay be sterically hindered in the presence of the anti-body. The results of this study allow us ... attachment of bacteria toFn, even in the presence of excess amounts of 15E11,is mediated by repeats that are not targeted by the mAb. Interestingly, the inhibitory effect of 15E11 on the attachment of ... cell shape and migration, and differentiation of many cell types [13].Fn is a dimer of two similar polypeptides held together by a pair of disulfide bonds at their C-termini. Itis one of the main...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... optimum, and a broadspeci®city w ith respect to the g lycone moiety of substrates.Human CBG hydrolyses synthetic aryl glycosides (including4-nitrophenyl and 4-methylumbelliferyl monoglycosides)[9], ... [the hydrolysis of 4NP-b-D-glucopyranoside (10 mM) was reduced by 98% in the presence of 4NP-b-D-mannopyranoside (10 mM)].Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG ... (1997) The crystal structures of Sinapis alba myro-sinase and a covalent glycosyl±enzyme intermediate provideinsights into the substrate recogn ition and active-site machinery of an S-glycosidase....
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Từ khóa: functional analysis of the cervicalfunctional analysis of intergenicbáo cáo y họcbáo cáo y học cổ truyềnmẫu báo cáo y học cổ truyềnbao cao y hoc colchicinphan ban luan trong bao cao y hoc co truyena functional analysis of the cameron site chipped stone assemblageissues which surfaced during ct reviews by recs that were included in the recs decision reportsidentification and analysis of the ethical scientific issues which surfaced during ct reviews by recs that were included in the recs decision reportsbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămbáo cáo y tế học đường năm 2012báo cáo y tế học đường trường mầm nonbiểu mẫu báo cáo y tế trường họcNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ