Báo cáo khoa học: P-Glycoprotein is localized in intermediate-density membrane microdomains distinct from classical lipid rafts and caveolar domains potx

... presen- ted in Fig. 1A indicate that Pgp is localized in mem- brane microdomains that are distinct from classical lipid rafts containing Src-family tyrosine kinases. The mem- brane microdomains containing ... FEBS 4937 P-Glycoprotein is localized in intermediate-density membrane microdomains distinct from classical lipid rafts and caveolar do...

Ngày tải lên: 16/03/2014, 23:20

... as insulin growth factor-binding proteins and osteopontin [25], and is unlike the complete modification reported for phos- phoproteins secreted from mammary and salivary glands [25]. Interestingly, ... FEBS endogenous inhibitor, a catalytic domain characteristic of serine proteases and a C-terminal Cys- and His-rich domain implicated in enzyme stability and protein– protein i...

Ngày tải lên: 16/03/2014, 06:20

... Sin3 and or Rpd3, rather than by regulating Sin3 ⁄ Rpd3 directly. A good candidate for a factor regulating Sin3 ⁄ Rpd3 binding is Stb1, because it binds to both Swi6 and Sin3 [20,21,32,34]. In ... astonishing precision of transcriptional timing observed in cell cycle regulated transcription in late G 1 . Materials and methods Strains and DNA Strains used in this study were d...

Ngày tải lên: 23/03/2014, 05:22

... (nuclei) staining; (B) Mitofluor Red 589 (mitochondria) stain- ing; (C) anti-His (exogenous PHB)-FITC stain- ing; (D) merge. The arrows indicate staining of His-tagged PHB in (C) or both PHB and Mitofluor ... Cave- olins and lipid rafts are involved in the internalization of various molecules [51,52]. Vessal et al. [29] identified EH domain 2 as a binding partner for PHB, and both...

Ngày tải lên: 29/03/2014, 08:20

... showed that the number of Six1-binding sites is at least three, whereas there is one Six4-binding site, indicating that the DNA-binding specificity of Six1 is distinct from that of Six4. Another member ... Six4, suggesting that the DNA-binding specificity of Six1 is distinct from that of Six4. This underlies the differential regulation of common target genes by Six1 and Six4....

Ngày tải lên: 07/03/2014, 17:20

... 1B), and showed five amino acid substitutions in the a-domain, one in the b-domain and conservation of all 21 cysteines. The CvMT-II subfamily is distinguished by the presence of only a -domains in ... sulfhydryl-rich metal-binding proteins with characteristic repeating cysteine motifs (Cys-X-Cys or Cys- X n -Cys) and a prolate ellipsoid shape containing single a- and b -domai...

Ngày tải lên: 30/03/2014, 13:20

... is the proximal axial ligand to the d 1 heme. Replacement of an equivalent His, His41, in NirF by Ala abolished binding of the heme to the protein. Known distal heme-binding residues, such as Met, ... a periplasmic protein and, hence, must be involved in a late stage of the cofac- tor biosynthesis. In vitro studies with purified NirF established that it could bind d 1 heme. It is co...

Ngày tải lên: 15/02/2014, 01:20

... data AcLDL 1 2 Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA CD36/cyclophilin mRNA FABP4/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA 3 4 3 ... still controversial, visfatin is thought to have insulin mimetic effects and, similarly to insulin, visfatin enhances glucose uptake by myocytes and adipocytes and...

Ngày tải lên: 06/03/2014, 22:21

... vector pQCXIN, pQCXIN-NANOGP8 and pQCXIN-NANOGP8-GFP using Lipofectamine TM 2000 according to the manufac- turer’s instructions. Stable clones were selected and isolated in media containing 500 ... by RT-PCR and inser- ted into pQCXIN between AgeI and PacI sites. The GFP and( or) NANOGP8 were(was) ligated into the pQCXIN vector to produce the pQCXIN-GFP, pQCXIN- NANOGP8, and pQCX...

Ngày tải lên: 07/03/2014, 12:20

... his3:hisG/his3::hisG trp1::hisG/trp1::hisG This study MR211 S1278b; MATa ura3–52 his3:hisG leu2::hisG ras2::kanMX MR298 S1278b; MATa/a ras2::kanMX/ras2::kanMX ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG ... his3:hisG/his3:hisG leu2::hisG leu)2::hisG This study AR2 S1278b; MATa/a sut2::S.pombe his5 + /sut2::S.pombe his5 + ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG/LEU2 TRP1/trp1::hisG T...

Ngày tải lên: 07/03/2014, 15:20