Báo cáo khoa học: Characterization of N-glycosylation consen

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

... SGEY C Fig. 1. Topological model of Kv3. 1 and amino acid sequences of the S1–S2 linker of Kvs. (A) Topology of a Kv3. 1 monomeric unit. Black circles represent the Asn of native N-glycosylation consensus ... that N-glycosylation in uences the open- ing of the voltage-dependent gate of Kv3. 1, suggesting that vacant sites alter the folding and matur...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus inﬂuenzae pptx

... GlycoformObserved Calculated 11 -OS -1 11- OS-2 11 -OS-3 16 -OS -1 1263.7 12 40.7 12 40.6 1 2 2 6 Hex 1 •Hep 3 •AnKdo-ol Hex1 14 67.8 14 44.8 14 44.7 7 18 19 9 Hex 2 •Hep 3 •AnKdo-ol Hex2 16 71. 9 16 48.9 16 48.8 ... response Linkage assignment LPS-OH (strain 11 ) LPS-OH (strain 16 ) 11 -OS -1 11- OS-1HF 11 -OS-2 11 -OS-3 16 -OS-1HF 16 -OS-2 2,3,4,6-Me 4 -Glc 1. 00...

Ngày tải lên : 16/03/2014, 16:20

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411
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... values (nmolÆmin )1 Æmg protein )1 ) ATPase None 2 41 437 393 400 l M Na 3 VO 4 18 1 10 6 335 10 0 n M baﬁlomycin 18 7 412 13 1 lgÆmL )1 oligomycin 16 3 418 325 Cytochrome c reductase – 15 8 15 32 Latent ... cyanidin; 10 0 nm bilirubin and 10 0 nm biliverdin. Under the condi- tions of the assay, bilirubin is freely soluble in the buffer [58]. The presence of t...

Ngày tải lên : 20/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... preparations of CD11b/ CD18 integrins. The mAbs to CD11a/CD18, CD11b/CD18 and ICAMs clearly inhibited the binding of ICAM L cells to coated CD18 integrins (data not presented). To examine the role of I ... domain in ICAM-2 seem to mediate CD11b/ CD18 binding [12 ]. Extensive work has been carried out to localize the ligand binding sites in the leukocyte b 2 integrins. The...

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... identi- ﬁed all the candidate residues involved in the binding of diiron (His22, His58, Glu62, His77, His 81, His 117 , Asp122) and the amino acids forming the hydrophobic pocket in which O 2 may bind (Ile25, ... from the PCR fragment ampliﬁed with the primers used in the cloning of MCA0 715 . The arrowhead indicates the 540 bp transcript, corresponding to the pr...

Ngày tải lên : 07/03/2014, 17:20

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501
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Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel speciﬁcity towards C-terminal glutamate residues pptx

... 2004, revised 19 March 2004, accepted 24 March 2004) Eur. J. Biochem. 2 71, 2000–2 011 (2004) Ó FEBS 2004 doi :10 .11 11/ j .14 32 -10 33.2004.0 411 3.x extracts of the webbing clothes moth, Tineola bisselliella. Soluble ... majority, and by 20 min all, of the original 50 kDa protein had been digested, with an increase in staining of the bands of  36 kDa and  13 kDa. On fu...

Ngày tải lên : 23/03/2014, 12:20

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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... K22 is missing (as in the K22V mutant), the fosfomycin binding/reaction with C 115 occurs only in the presence of UDPNAG as the substrate promotes the interaction of R120 and fosfomycin in order ... concentration. The dissociation constants, K d values, enthalpies of binding and stoichiometries were determined from the binding isotherm by ﬁtting a 1 : 1 binding m...

Ngày tải lên : 19/02/2014, 13:20

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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... 11 480.5 apo-AcpK 10 5 61. 5 10 5 61. 0 holo-AcpK 10 9 01. 6 10 9 01. 0 decanoyl-DptF 13 986.9 13 986.9 decanoyl-LipD 11 635.6 11 635.7 decanoyl-AcpK 11 056.7 11 056.8 M. Wittmann et al. Lipidation of ... ()) indicates the reaction without Sfp. m/z 13 4 91. 6 apo-DptF + Na + Ka Relative abundance 13 200 13 300 13 400 13 500 13 600 13 700 13 800 13 900 13 600 13 700...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Deamidation of labile asparagine residues in the autoregulatory sequence of human phenylalanine hydroxylase potx

... H 4 biopterin V max a (nmolÆmin )1 Æmg )1 ) ½S 0:5 a (l M ) k cat /[S] 0.5 (l M Æmin )1 ) h Substrate inhibition V max (nmolÆmin )1 Æmg )1 ) K m b (l M ) Wt (2 h) 3484 ± 16 2 238 ± 18 0.72 1. 5 ± 0 .1 ‚ 5325 ± 13 7 31 ± 3 Wt (24 h) 4946 ± 2 81 135 ± 11 1. 83 1. 9 ± 0 .1 + 6392 ... 310 7.470, 310 8.474 and 310 9.476) of decreasing intensity on deconvolution of the...

Ngày tải lên : 08/03/2014, 08:20

10
450
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Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

... of the MR1 51- derived strain with the crtIa deletion (MR8 41) com- pletely lose the red color typical of colonies from the parental strain, suggesting that the absence of CrtIa blocks the synthesis ... 4307 in- frame deletion of crtIa in M. xanthus strain MR1 51. This strain contains a mutation, carR3, which renders the expression of the carB operon independent...

Ngày tải lên : 16/03/2014, 05:20

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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3 1 channel pot

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus inﬂuenzae pptx